scholarly journals Epigallocatechin-3-Gallate Suppresses Vasculogenic Mimicry through Inhibiting the Twist/VE-Cadherin/AKT Pathway in Human Prostate Cancer PC-3 Cells

2020 ◽  
Vol 21 (2) ◽  
pp. 439 ◽  
Author(s):  
Changhwan Yeo ◽  
Deok-Soo Han ◽  
Hyo-Jeong Lee ◽  
Eun-Ok Lee

Vasculogenic mimicry (VM) is the alternative process of forming vessel-like networks by aggressive tumor cells, and it has an important role in tumor survival, growth, and metastasis. Epigallocatechin-3-gallate (EGCG) is well known to have diverse bioactivities including anti-cancer effects. However, the efficacy of EGCG on VM is elusive. In this study, we explored whether and how EGCG affects VM in human prostate cancer (PCa) PC-3 cells. Cell viability was measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Invasive and VM formation abilities were assessed by an invasion assay and a three-dimensional (3D) culture VM tube formation assay, respectively. Western blots were carried out. An immunofluorescence assay was performed to detect nuclear twist expression. EGCG effectively inhibited the invasive ability, as well as tubular channel formation, without affecting cell viability. EGCG significantly downregulated the expression of vascular endothelial cadherin (VE-cadherin) and its transcription factor, twist, N-cadherin, vimentin, phosphor-AKT, and AKT, but not phospho-erythropoietin-producing hepatocellular receptor A2 (EphA2) and EphA2. In addition, EGCG diminished the nuclear localization of twist. Treatment with SC79, an AKT activator, effectively rescued EGCG-inhibited VM formation. These results demonstrated for the first time that EGCG causes marked suppression of VM through inhibiting the twist/VE-cadherin/AKT pathway in human PCa PC-3 cells.

2002 ◽  
Vol 30 (2) ◽  
pp. 116-121 ◽  
Author(s):  
Scott Davidson ◽  
Dan Milanesa ◽  
Camille Mallouh ◽  
Muhammad Choudhury ◽  
Hiroshi Tazaki ◽  
...  

Oncoscience ◽  
2019 ◽  
Vol 6 (5-6) ◽  
pp. 317-336 ◽  
Author(s):  
Abdulrahman Alwhaibi ◽  
Ravindra Kolhe ◽  
Fei Gao ◽  
Ewan K. Cobran ◽  
Payaningal R. Somanath

2018 ◽  
Vol 14 (58) ◽  
pp. 477 ◽  
Author(s):  
Palanisamy Arulselvan ◽  
Jiechang Ju ◽  
Sivapragasam Gothai ◽  
Mohadeseh Hasanpourghadi ◽  
AnmarA Nasser ◽  
...  

Life Sciences ◽  
2009 ◽  
Vol 85 (11-12) ◽  
pp. 421-430 ◽  
Author(s):  
V. Alonso ◽  
F.C. Pérez-Martínez ◽  
F.J. Calahorra ◽  
P. Esbrit

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