scholarly journals Synergistic Effect of Beauveria bassiana and Trichoderma asperellum to Induce Maize (Zea mays L.) Defense against the Asian Corn Borer, Ostrinia furnacalis (Lepidoptera, Crambidae) and Larval Immune Response

2020 ◽  
Vol 21 (21) ◽  
pp. 8215
Author(s):  
Raufa Batool ◽  
Muhammad Jawad Umer ◽  
Yangzhou Wang ◽  
Kanglai He ◽  
Tiantao Zhang ◽  
...  
Keyword(s):  
Immune Response ◽  
Synergistic Effect ◽  
Beauveria Bassiana ◽  
Larval Mortality ◽  
Seed Coating ◽  
Trichoderma Asperellum ◽  
Ostrinia Furnacalis ◽  
Transcriptomic Response ◽  
Corn Borer ◽  
Fungal Isolates

Ostrinia furnacalis, is the major pest of maize causing significant yield losses. So far, many approaches have been used to increase the virulence of entomopathogenic fungal isolates. The current study is an attempt to estimate synergistic effect of Beauveria bassiana and Trichoderma asperellum in order to explore larval immune response through RNA sequencing and differentially expression analysis. In vivo synergism was examined in seven proportions (B. bassiana: T. asperellum = 1:1, 1:2, 1:3, 1:4, 4:1, 3:1, 2:1) and in the in vitro case, two inoculation methods were applied: seed coating and soil drenching. Results revealed significant decrease in plant damage and high larval mortality in fungal treatments. Fungal isolates mediated the plant defense by increasing proline, superoxide dismutase (SOD), peroxidase (POD), polyphenol oxidase (PPO) and protease activities. Seed coating method was proved to be the most effective in case of maize endophytic colonization. In total, 59 immune-related differentially expressed genes DEGs were identified including, cytochrome P450, heat shock protein, ABC transporter, cadherin, peptidoglycan recognition protein (PGRP), cuticlular protein, etc. Further, transcriptomic response was confirmed by qRT-PCR. Our results concluded that, coculture of B. bassiana and T. asperellum has the synergistic potential to suppress the immune response of O. furnacalis and can be used as sustainable approach to induce plant resistance through activation of defense-related enzymes.

Ecological control of the Asian corn borer, Ostrinia furnacalis (Guenée) by two cloned Beauveria bassiana strains

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
冯树丹 FENG Shudan ◽  
李晓慧 LI Xiaohui ◽  
汪洋洲 WANG Yangzhou ◽  
张军 ZHANG Jun ◽  
徐文静 XU Wenjing ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Ostrinia Furnacalis ◽  
Corn Borer ◽  
Ecological Control ◽  
Asian Corn Borer

A PHENOLOGY MODEL AND FIELD EVALUATION OF BEAUVERIA BASSIANA (BALS.) VUILLEMIN (DEUTEROMYCOTINA: HYPHOMYCETES) MYCOSIS OF THE EUROPEAN CORN BORER, OSTRINIA NUBILALIS (HBN.) (LEPIDOPTERA: PYRALIDAE)

1988 ◽  
Vol 120 (2) ◽  
pp. 133-144 ◽  
Author(s):  
Ziding Feng ◽  
Raymond I. Carruthers ◽  
Timothy S. Larkin ◽  
Donald W. Roberts
Keyword(s):  
Beauveria Bassiana ◽  
Ostrinia Nubilalis ◽  
European Corn Borer ◽  
Larval Mortality ◽  
Field Evaluation ◽  
Time Model ◽  
Corn Borer ◽  
Corn Borers ◽  
Phenology Model ◽  
Validation Experiments

AbstractA simulation model was developed to predict the timing of European corn borer, Ostrinia nubilalis (Hbn.), larval mortality following exposure to Beauveria bassiana (Bals.) Vuillemin conidia. Model response was compared with laboratory and field data collected specifically for model validation. In laboratory validation experiments, four conidial doses of a B. bassiana isolate collected from the People’s Republic of China were applied to European corn borer larvae which were then incubated at fluctuating temperatures. Simulation results compared favorably with fungal development and mycosis under laboratory conditions. Field validation experiments showed that applications of B. bassiana to European corn borer neonates in whorl-stage corn resulted in mortality levels over 60% when conidia and European corn borers were placed on the plants on the same day. When B. bassiana conidia were placed on plants prior to European corn borers, mortality decreased linearly with time. Model predictions of the timing of European corn borer mortality were found to be accurate when B. bassiana conidia were placed on corn plants at the same time or prior to European corn borers. When European corn borers were placed on plants prior to conidia, the model underestimated the time to European corn borer death. Lack of model fit in this situation is thought to be due to delayed host and pathogen interactions on the corn plant rather than poor prediction of the physiological development of the disease.

scholarly journals Efficacy of a Beauveria bassiana strain, Bacillus thuringiensis and their combination against the tomato leafminer Tuta absoluta

2017 ◽  
Vol 25 (2) ◽  
pp. 23 ◽  
Author(s):  
Despoina Tsoulnara ◽  
Gordon Port
Keyword(s):  
Bacillus Thuringiensis ◽  
Synergistic Effect ◽  
Beauveria Bassiana ◽  
Larval Mortality ◽  
Tuta Absoluta ◽  
Recommended Dose ◽  
Tomato Leafminer ◽  
Single Leaf ◽  
Combined Use ◽  
Good Potential

A single leaf bioassay was developed to investigate the entomopathogenic efficacy of a Beauveria bassiana strain, Bacillus thuringiensis (Costar®) and their interactions on larval mortality of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae). Tomato leaves were removed from the plants and sprayed with manufacturers’ highest field recommended concentrations for tomato crops before or after infesting the leaves with the larvae. Third instar larvae proved the most susceptible, while susceptibility was lower in the second instar larvae. The combined use showed a higher potential indicating a positive synergistic effect. In addition, treated leaves were sprayed directly with concentrations of 0.0, 0.05, 0.10, 0.15 and 0.20 mL/L of B. bassiana. The efficacy was higher for the tested concentration of 0.20 mL/L that is higher than the recommended dose. The present study suggests that those bioinsecticides have a good potential in the control of T. absoluta.

PLA 2 mediates the innate immune response in Asian corn borer, Ostrinia furnacalis

2021 ◽  
Author(s):  
Jia‐Yue Ji ◽  
Zhao‐Hua Yin ◽  
Sha‐Sha Zhang ◽  
Dong‐Xu Shen ◽  
Chun‐Ju An
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Innate Immune ◽  
Ostrinia Furnacalis ◽  
Corn Borer ◽  
Asian Corn Borer

scholarly journals A Short-Type Peptidoglycan Recognition Protein 1 (PGRP1) Is Involved in the Immune Response in Asian Corn Borer, Ostrinia furnacalis (Guenée)

2021 ◽  
Vol 22 (15) ◽  
pp. 8198
Author(s):  
Dongxu Shen ◽  
Jiayue Ji ◽  
Shasha Zhang ◽  
Jiahui Liu ◽  
Chunju An
Keyword(s):  
Immune Response ◽  
Micrococcus Luteus ◽  
Binding Activity ◽  
Ostrinia Furnacalis ◽  
Corn Borer ◽  
Peptidoglycan Recognition Protein ◽  
Asian Corn Borer ◽  
Recognition Protein ◽  
Fat Bodies ◽  
Insect Immune Response

The insect immune response is initiated by the recognition of invading microorganisms. Peptidoglycan recognition proteins (PGRPs) function primarily as pattern recognition receptors by specifically binding to peptidoglycans expressed on microbial surfaces. We cloned a full-length cDNA for a PGRP from the Asian corn borer Ostrinia furnacalis (Guenée) and designated it as PGRP1. PGRP1 mRNA was mainly detected in the fat bodies and hemocytes. Its transcript levels increased significantly upon bacterial and fungal challenges. Purified recombinant PGRP1 exhibited binding activity to the gram-positive Micrococcus luteus, gram-negative Escherichia coli, entomopathogenic fungi Beauveria bassiana, and yeast Pichia pastoris. The binding further induced their agglutination. Additionally, PGRP1 preferred to bind to Lys-type peptidoglycans rather than DAP-type peptidoglycans. The addition of recombinant PGRP1 to O. furnacalis plasma resulted in a significant increase in phenoloxidase activity. The injection of recombinant PGRP1 into larvae led to a significantly increased expression of several antimicrobial peptide genes. Taken together, our results suggest that O. furnacalis PGRP1 potentially recognizes the invading microbes and is involved in the immune response in O. furnacalis.

scholarly journals Transcriptomic response of Anopheles gambiae sensu stricto mosquito larvae to Curry tree (Murraya koenigii) phytochemicals

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Clarence M. Mang’era ◽  
Fathiya M. Khamis ◽  
Erick O. Awuoche ◽  
Ahmed Hassanali ◽  
Fidelis Levi Odhiambo Ombura ◽  
...  
Keyword(s):  
Immune Response ◽  
Anopheles Gambiae ◽  
Growth And Development ◽  
Leaf Extract ◽  
Expression Profiles ◽  
Sensu Stricto ◽  
Mosquito Larvae ◽  
Insect Vector ◽  
Murraya Koenigii ◽  
Transcriptomic Response

Abstract Background Insect growth regulators (IGRs) can control insect vector populations by disrupting growth and development in juvenile stages of the vectors. We previously identified and described the curry tree (Murraya koenigii (L.) Spreng) phytochemical leaf extract composition (neplanocin A, 3-(1-naphthyl)-l-alanine, lumiflavine, terezine C, agelaspongin and murrayazolinol), which disrupted growth and development in Anopheles gambiae sensu stricto mosquito larvae by inducing morphogenetic abnormalities, reducing locomotion and delaying pupation in the mosquito. Here, we attempted to establish the transcriptional process in the larvae that underpins these phenotypes in the mosquito. Methods We first exposed third-fourth instar larvae of the mosquito to the leaf extract and consequently the inherent phytochemicals (and corresponding non-exposed controls) in two independent biological replicates. We collected the larvae for our experiments sampled 24 h before peak pupation, which was 7 and 18 days post-exposure for controls and exposed larvae, respectively. The differences in duration to peak pupation were due to extract-induced growth delay in the larvae. The two study groups (exposed vs control) were consequently not age-matched. We then sequentially (i) isolated RNA (whole larvae) from each replicate treatment, (ii) sequenced the RNA on Illumina HiSeq platform, (iii) performed differential bioinformatics analyses between libraries (exposed vs control) and (iv) independently validated the transcriptome expression profiles through RT-qPCR. Results Our analyses revealed significant induction of transcripts predominantly associated with hard cuticular proteins, juvenile hormone esterases, immunity and detoxification in the larvae samples exposed to the extract relative to the non-exposed control samples. Our analysis also revealed alteration of pathways functionally associated with putrescine metabolism and structural constituents of the cuticle in the extract-exposed larvae relative to the non-exposed control, putatively linked to the exoskeleton and immune response in the larvae. The extract-exposed larvae also appeared to have suppressed pathways functionally associated with molting, cell division and growth in the larvae. However, given the age mismatch between the extract-exposed and non-exposed larvae, we can attribute the modulation of innate immune, detoxification, cuticular and associated transcripts and pathways we observed to effects of age differences among the larvae samples (exposed vs control) and to exposures of the larvae to the extract. Conclusions The exposure treatment appears to disrupt cuticular development, immune response and oxidative stress pathways in Anopheles gambiae s.s larvae. These pathways can potentially be targeted in development of more efficacious curry tree phytochemical-based IGRs against An. gambiae s.s mosquito larvae.

Sign in / Sign up

Export Citation Format

Share Document