scholarly journals Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum

2020 ◽  
Vol 21 (24) ◽  
pp. 9570
Author(s):  
Piotr Wilk ◽  
Katarzyna Kuśka ◽  
Elżbieta Wątor ◽  
Piotr H. Małecki ◽  
Klaudia Woś ◽  
...  
Keyword(s):  
Organic Compound ◽  
Crystal Structures ◽  
Filamentous Fungus ◽  
Glycerol Kinase ◽  
Glycerol Metabolism ◽  
Catabolic Pathway ◽  
Alternative Source ◽  
Chaetomium Thermophilum ◽  
Structural Insights

Glycerol is an organic compound that can be utilized as an alternative source of carbon by various organisms. One of the ways to assimilate glycerol by the cell is the phosphorylative catabolic pathway in which its activation is catalyzed by glycerol kinase (GK) and glycerol-3-phosphate (G3P) is formed. To date, several GK crystal structures from bacteria, archaea, and unicellular eukaryotic parasites have been solved. Herein, we present a series of crystal structures of GK from Chaetomium thermophilum (CtGK) in apo and glycerol-bound forms. In addition, we show the feasibility of an ADP-dependent glucokinase (ADPGK)-coupled enzymatic assay to measure the CtGK activity. New structures described in our work provide structural insights into the GK catalyzed reaction in the filamentous fungus and set the foundation for understanding the glycerol metabolism in eukaryotes.

Structural insights into the mechanism of calmodulin binding to death receptors

2014 ◽  
Vol 70 (6) ◽  
pp. 1604-1613 ◽  
Author(s):  
Peng Cao ◽  
Wenting Zhang ◽  
Wenjun Gui ◽  
Yuhui Dong ◽  
Tao Jiang ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Binding Sites ◽  
Death Receptors ◽  
Calcium Sensor ◽  
Death Domain ◽  
Therapeutic Peptide ◽  
Calmodulin Binding ◽  
Open Conformation ◽  
Structural Insights

The death receptors Fas, p75NTRand DR6 are key components of extrinsically activated apoptosis. Characterization of how they interact with the adaptors is crucial in order to unravel the signalling mechanisms. However, the exact conformation that their intracellular death domain adopts upon binding downstream partners remains unclear. One model suggests that it adopts a typical compact fold, whilst a second model proposed an open conformation. Calmodulin (CaM), a major calcium sensor, has previously been reported to be one of the Fas adaptors that modulate apoptosis. This work reports that CaM also binds directly to the death domains of p75NTRand DR6, indicating that it serves as a common modulator of the death receptors. Two crystal structures of CaM in complexes with the corresponding binding regions of Fas and p75NTRare also reported. Interestingly, the precise CaM-binding sites were mapped to different regions: helix 1 in Fas and helix 5 in p75NTRand DR6. A novel 1–11 motif for CaM binding was observed in p75NTR. Modelling the complexes of CaM with full-length receptors reveals that the opening of the death domains would be essential in order to expose their binding sites for CaM. These results may facilitate understanding of the diverse functional repertoire of death receptors and CaM and provide further insights necessary for the design of potential therapeutic peptide agents.

Biochemical characterization of the dicing activity of Dicer-like 2 in the model filamentous fungus Neurospora crassa

2021 ◽  
Vol 146 ◽  
pp. 103488
Author(s):  
Midori Tabara ◽  
Hisashi Koiwa ◽  
Nobuhiro Suzuki ◽  
Toshiyuki Fukuhara
Keyword(s):  
Neurospora Crassa ◽  
Filamentous Fungus ◽  
Biochemical Characterization

scholarly journals Structural insights into targeting of the colchicine binding site by ELR510444 and parbendazole to achieve rational drug design

RSC Advances ◽  
2021 ◽  
Vol 11 (31) ◽  
pp. 18938-18944
Author(s):  
Jia-Hong Lei ◽  
Ling-Ling Ma ◽  
Jing-Hong Xian ◽  
Hai Chen ◽  
Jian-Jian Zhou ◽  
...  
Keyword(s):  
Drug Design ◽  
Binding Site ◽  
Crystal Structures ◽  
Rational Drug Design ◽  
Colchicine Binding Site ◽  
Rational Drug ◽  
Structural Insights

Crystal structures of tubulin complexed with ELR510444 and parbendazole facilitate the design of novel colchicine binding site inhibitors.

scholarly journals Characterization of an l-Ascorbate Catabolic Pathway with Unprecedented Enzymatic Transformations

2020 ◽  
Vol 142 (4) ◽  
pp. 1657-1661 ◽  
Author(s):  
Tyler M. M. Stack ◽  
Katelyn N. Morrison ◽  
Thomas M. Dettmer ◽  
Brendan Wille ◽  
Chan Kim ◽  
...  
Keyword(s):  
Catabolic Pathway

Distorted chiolite crystal structures of α-Na5M3F14 (M=Cr,Fe,Ga) studied by X-ray powder diffraction

2003 ◽  
Vol 18 (2) ◽  
pp. 128-134 ◽  
Author(s):  
A. Le Bail ◽  
A.-M. Mercier
Keyword(s):  
Crystal Structures ◽  
Powder Diffraction ◽  
Room Temperature ◽  
Rietveld Refinements ◽  
Space Group ◽  
X Ray ◽  
Precise Characterization

The crystal structures of the chiolite-related room temperature phases α-Na5M3F14 (MIII=Cr,Fe,Ga) are determined. For all of them, the space group is P21/n, Z=2; a=10.5096(3) Å, b=7.2253(2) Å, c=7.2713(2) Å, β=90.6753(7)° (M=Cr); a=10.4342(7) Å, b=7.3418(6) Å, c=7.4023(6) Å, β=90.799(5)° (M=Fe), and a=10.4052(1) Å, b=7.2251(1) Å, c=7.2689(1), β=90.6640(4)° (M=Ga). Rietveld refinements produce final RF factors 0.036, 0.033, and 0.035, and RWP factors, 0.125, 0.116, and 0.096, for MIII=Cr, Fe, and Ga, respectively. The MF6 polyhedra in the defective isolated perovskite-like layers deviate very few from perfect octahedra. Subtle octahedra tiltings lead to the symmetry decrease from the P4/mnc space group adopted by the Na5Al3F14 chiolite aristotype to the P21/n space group adopted by the title series. Facile twinning precluded till now the precise characterization of these compounds.

Glycerol metabolism in rabbits

1968 ◽  
Vol 46 (9) ◽  
pp. 1107-1114 ◽  
Author(s):  
Jean Himms-Hagen
Keyword(s):  
Turnover Rate ◽  
Glycerol Kinase ◽  
Rabbit Liver ◽  
Constant Infusion ◽  
Glycerol Dehydrogenase ◽  
Glycerol Metabolism ◽  
Utilization Rate ◽  
Glycerol Concentration ◽  
Glycerol Utilization ◽  
Fractional Turnover

The endogenous rate of glycerol production in rabbits was measured by several techniques: constant infusion of 1,3-14C-glycerol or 2-3H-glycerol or unlabeled glycerol; single injection of 1,3-14C-glycerol or 2-3H-glycerol or unlabeled glycerol. The rate was 5.5–11.6 μmoles/kg per minute (9 rabbits). The mean fractional turnover rate was 0.0585 ± 0.0052. During infusion of noradrenaline together with 3H-glycerol, the fractional turnover rate was no different from that in the absence of noradrenaline. The maximum utilization rate of glycerol was 28.1 ± 1.40 μmoles/kg per minute. The glycerol space was 58.1% of body weight. The relationship of glycerol concentration to rate of glycerol utilization in the intact rabbit suggests the existence of an enzyme with a KM for glycerol of 0.33 × 10−3 M; the glycerol kinase of rabbit liver was found to have a KM for glycerol of 0.29 × 10−3 M. This enzyme could account for the disappearance of glycerol in the intact animal except that its Vmax is only 4% of that expected. Possible reasons for this are discussed. A glycerol dehydrogenase with a Vmax similar to that of the glycerol kinase also exists in rabbit liver; its KM for glycerol is so high (0.5 M) that it is unlikely to play a significant role in glycerol metabolism in the normal rabbit.

Characterization of three multicopper oxidases in the filamentous fungus Podospora anserina : A new role of an ABR1-like protein in fungal development?

2018 ◽  
Vol 116 ◽  
pp. 1-13 ◽  
Author(s):  
Ning Xie ◽  
Gwenaël Ruprich-Robert ◽  
Philippe Silar ◽  
Eric Herbert ◽  
Roselyne Ferrari ◽  
...  
Keyword(s):  
Filamentous Fungus ◽  
Podospora Anserina ◽  
Multicopper Oxidases ◽  
Fungal Development
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