Overview of the Therapeutic Potential of Aptamers Targeting Coagulation Factors

Aptamers are single-stranded DNA or RNA sequences that bind target molecules with high specificity and affinity. Aptamers exhibit several notable advantages over protein-based therapeutics. Aptamers are non-immunogenic, easier to synthesize and modify, and can bind targets with greater affinity. Due to these benefits, aptamers are considered a promising therapeutic candidate to treat various conditions, including hematological disorders and cancer. An active area of research involves developing aptamers to target blood coagulation factors. These aptamers have the potential to treat cardiovascular diseases, blood disorders, and cancers. Although no aptamers targeting blood coagulation factors have been approved for clinical use, several aptamers have been evaluated in clinical trials and many more have demonstrated encouraging preclinical results. This review summarized our knowledge of the aptamers targeting proteins involved in coagulation, anticoagulation, fibrinolysis, their extensive applications as therapeutics and diagnostics tools, and the challenges they face for advancing to clinical use.

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Natural Oestrogen in the Female Climacteric - Influence on Blood Coagulation and Fibrinolysis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648686 ◽

1978 ◽

Vol 40 (02) ◽

pp. 532-541 ◽

Cited By ~ 2

Author(s):

Anders Lagrelius ◽

Nils-Olov Lunell ◽

Margareta Blombäck

Keyword(s):

Blood Coagulation ◽

Antithrombin Iii ◽

Coagulation Factors ◽

Control Group ◽

Blood Coagulation Factors ◽

Excessive Bleeding ◽

Oestrone Sulphate ◽

Menopausal Women ◽

History Of ◽

Coagulation And Fibrinolysis

SummaryThe aim of the present study was to investigate the effect on blood coagulation and fibrinolysis of a natural oestrogen preparation, piperazine oestrone sulphate, prospectively in menopausal women. Scopolamine was given to the control group.The women were investigated before and during treatment with regard to factors VIII, VII, X, V, fibrinopeptide A, antithrombin III, plasminogen, rapid antiplasmin and α1-antitrypsin. There was no significant change towards hypercoagulability or decreased fibrinolysis in any group. In the oestrogen group, however, a tendency towards an increased level of plasminogen and a decreased level of antiplasmin was demonstrated. In the scopolamine group there was an unexpected fall in factors X and V and also in plasminogen and α1,-antitrypsin. A low level of some blood coagulation factors in some of the women before treatment is somewhat astonishing; none of them had any history of excessive bleeding.

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STEROIDSTRUKTUR UND LEBERTOXIZITÄT

Acta Endocrinologica ◽

10.1530/acta.0.0540073 ◽

1967 ◽

Vol 54 (1) ◽

pp. 73-84 ◽

Cited By ~ 7

Author(s):

H. L. Krüskemper ◽

G. Noell

Keyword(s):

Alkaline Phosphatase ◽

Liver Function ◽

Blood Coagulation ◽

Electrophoretic Pattern ◽

Coagulation Factors ◽

Blood Coagulation Factors ◽

Methyl Group ◽

Liver Functions ◽

Male Subjects ◽

Androstane Derivatives

ABSTRACT In male subjects investigations have been carried out regarding the effect of C1- and C17-methylated androstane derivatives (20 mg per day, orally, two weeks) on liver functions (parameters: activities of GPT, GOT, alkaline phosphatase and cholinesterase in serum; electrophoretic pattern; blood coagulation factors V, VII, X and prothrombin; BSP-retention). In addition to the well known hepatotropic action of 17α-alkylated C-19-steroids a quasi-axial 1α-methyl configuration (in 1α-methylandrost-2-en-17β-ol) definitely increased BSP-retention and several coagulation factors. These steroid effects decreased gradually when a methyl group was introduced in C1 equatorially (1-methylandrost-1-en-17β-ol-3-one) or quasi-equatorially (1β-methylandrost-2-en-17β-ol), the latter compound completely lacking from any influence on parameters of liver function under investigation.

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Localization of the structural difference between bovine blood coagulation factors X1 and X2 to tyrosine 18 in the activation peptide.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)35614-4 ◽

1986 ◽

Vol 261 (9) ◽

pp. 4008-4014 ◽

Cited By ~ 2

Author(s):

T Morita ◽

C M Jackson

Keyword(s):

Blood Coagulation ◽

Structural Difference ◽

Coagulation Factors ◽

Bovine Blood ◽

Blood Coagulation Factors ◽

Activation Peptide

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EXPRESSION IN ONTOGENESIS OF HUMAN BLOOD COAGULATION FACTORS

10.1055/s-0038-1644610 ◽

1987 ◽

Author(s):

H J Hassan ◽

A Leonardi ◽

C Chelucci ◽

R Guerriero ◽

P M Mannucci ◽

...

Keyword(s):

Blood Coagulation ◽

Protein C ◽

Antithrombin Iii ◽

Liver Homogenate ◽

Coagulation Factor ◽

Coagulation Factors ◽

Factor Ix ◽

Adult Liver ◽

Blood Coagulation Factors ◽

Guanidine Isothiocyanate

We have analyzed the expression of several blood coagulation factors (IX, VIII, X, fibrinogen chains) and inhibitors (antithrombin III, protein C) in human embryonic and fetal livers, obtained from legal abortions at 6-11 week post-conception. The age was established by morphologic staging and particularly crown-rump lenght measurement.Total cellular RNA was isolated from partially purified hepatocytes or total liver homogenate using the guanidine isothiocyanate method. Poly(A)+ RNA was selected by oligodT cellulose chromatography. The size and the number of the embryonic and fetal transcripts are equivalent to those observed in adult liver, as evaluated by Northern blot analysis of total or poly(A)+ RNA hybridized to human cDNA probes.The level of coagulation factor transcripts in embryonic and fetal liver was evaluated by dot hybridization of total RNA (0.5-10 ug), as compared to RNA extracted from normal adult liver biopsies. The expression of blood coagulation factors in embryos is generally reduced for all factors, but at a different degree. In 5-11 wk liver, the level of factor IX is 5-10% of that observed in adults, while fibrinogen, protein C, antithrombin III RNA level rises from 25 to 50% and factor X is expressed at a level comparable to that observed in adult liver.We conclude that during these stages of development blood coagulation factors are expressed according to three different time, curves, possibly due to the effect of different types of regulatory mechanisms.

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