scholarly journals GhTBL34 Is Associated with Verticillium Wilt Resistance in Cotton

2021 ◽  
Vol 22 (17) ◽  
pp. 9115
Author(s):  
Yunlei Zhao ◽  
Huijuan Jing ◽  
Pei Zhao ◽  
Wei Chen ◽  
Xuelin Li ◽  
...  
Keyword(s):  
Cell Wall ◽  
Verticillium Wilt ◽  
Protein Sequences ◽  
Disease Index ◽  
Cell Wall Polysaccharides ◽  
Yield And Quality ◽  
Sequence Variations ◽  
Susceptible Allele ◽  
Exogenous Treatment

Verticillium wilt (VW) is a typical fungal disease affecting the yield and quality of cotton. The Trichome Birefringence-Like protein (TBL) is an acetyltransferase involved in the acetylation process of cell wall polysaccharides. Up to now, there are no reports on whether the TBL gene is related to disease resistance in cotton. In this study, we cloned a cotton TBL34 gene located in the confidence interval of a major VW resistance quantitative trait loci and demonstrated its relationship with VW resistance in cotton. Analyzing the sequence variations in resistant and susceptible accessions detected two elite alleles GhTBL34-2 and GhTBL34-3, mainly presented in resistant cotton lines whose disease index was significantly lower than that of susceptible lines carrying the allele GhTBL34-1. Comparing the TBL34 protein sequences showed that two amino acid differences in the TBL (PMR5N) domain changed the susceptible allele GhTBL34-1 into the resistant allele GhTBL34-2 (GhTBL34-3). Expression analysis showed that the TBL34 was obviously up-regulated by infection of Verticillium dahliae and exogenous treatment of ethylene (ET), and salicylic acid (SA) and jasmonate (JA) in cotton. VIGS experiments demonstrated that silencing of TBL34 reduced VW resistance in cotton. We deduced that the TBL34 gene mediating acetylation of cell wall polysaccharides might be involved in the regulation of resistance to VW in cotton.

scholarly journals Overexpression, crystallization and preliminary X-ray characterization ofRuminococcus flavefaciensscaffoldin C cohesin in complex with a dockerin from an uncharacterized CBM-containing protein

2014 ◽  
Vol 70 (8) ◽  
pp. 1061-1064 ◽  
Author(s):  
Pedro Bule ◽  
Vered Ruimy-Israeli ◽  
Vânia Cardoso ◽  
Edward A. Bayer ◽  
Carlos M. G. A. Fontes ◽  
...  
Keyword(s):  
Cell Wall ◽  
Protein Interactions ◽  
Plant Cell Wall ◽  
Biochemical Characterization ◽  
Anaerobic Bacteria ◽  
Energy Integration ◽  
Protein Protein Interactions ◽  
Cell Wall Polysaccharides ◽  
Multienzyme Complexes

Cellulosomes are massive cell-bound multienzyme complexes tethered by macromolecular scaffolds that coordinate the efforts of many anaerobic bacteria to hydrolyze plant cell-wall polysaccharides, which are a major untapped source of carbon and energy. Integration of cellulosomal components occursviahighly ordered protein–protein interactions between cohesin modules, located in the scaffold, and dockerin modules, found in the enzymes and other cellulosomal proteins. The proposed cellulosomal architecture forRuminococcus flavefaciensstrain FD-1 consists of a major scaffoldin (ScaB) that acts as the backbone to which other components attach. It has nine cohesins and a dockerin with a fused X-module that binds to the cohesin on ScaE, which in turn is covalently attached to the cell wall. The ScaA dockerin binds to ScaB cohesins allowing more carbohydrate-active modules to be assembled. ScaC acts as an adaptor that binds to both ScaA and selected ScaB cohesins, thereby increasing the repertoire of dockerin-bearing proteins that integrate into the complex. In previous studies, a screen for novel cohesin–dockerin complexes was performed which led to the identification of a total of 58 probable cohesin–dockerin pairs. Four were selected for subsequent structural and biochemical characterization based on the quality of their expression and the diversity in their specificities. One of these is C12D22, which comprises the cohesin from the adaptor ScaC protein bound to the dockerin of a CBM-containing protein. This complex has been purified and crystallized, and data were collected to resolutions of 2.5 Å (hexagonal,P65), 2.16 Å (orthorhombic,P212121) and 2.4 Å (orthorhombic,P21212) from three different crystalline forms.

scholarly journals The effects of short and long shading, applied during different stages of growth, on the development, productivity and quality of forage maize (Zea mays L.).

1983 ◽  
Vol 31 (2) ◽  
pp. 101-124
Author(s):  
P.C. Struik
Keyword(s):  
Cell Wall ◽  
Cell Walls ◽  
Field Experiments ◽  
Zea Mays L ◽  
Vegetative Development ◽  
Forage Maize ◽  
Stages Of Growth ◽  
Yield And Quality ◽  
Stover Yield

In 2 field experiments, shading which differed in duration and date of initiation was applied to normal stands of forage maize cv. LG11. Short shading during vegetative development affected leaf area, plant ht., stem thickness and reproductive development but final effects on DM yield and quality were small. Short shading during silking drastically reduced ear size and final ear yield. Although the deleterious effect on ear yield was partly compensated for by higher stover yield, productivity was low after the shading tents were removed. Digestibility was also greatly reduced because production of total DM was affected more than production of partly indigestible cell walls. Short shading soon after silking curtailed cell-wall formation more than DM production and crop digestibility was not adversely affected. Reduction in DM production remained large especially in the ear because of grain abortion. Shading after grain set stimulated the depletion of short carbohydrates in the stover and slowed down the decrease in the cell wall content of the whole crop. Crops shaded for long periods yielded more than expected on the basis of the short treatments. Long shading treatments lasted until final sampling and the earlier a long treatment was initiated, the greater the reduction in yield. The same was true for whole-crop digestibility, except in the earliest shading treatment in which poor vegetative development accompanied poor ear development. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals The Respiratory Burst Oxidase Homolog Protein D (GhRbohD) Positively Regulates the Cotton Resistance to Verticillium dahliae

2021 ◽  
Vol 22 (23) ◽  
pp. 13041
Author(s):  
Wanting Huang ◽  
Yalin Zhang ◽  
Jinglong Zhou ◽  
Feng Wei ◽  
Zili Feng ◽  
...  
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Time Course ◽  
Ros Production ◽  
Yield And Quality ◽  
Respiratory Burst Oxidase

Verticillium wilt, mainly caused by a soil-inhabiting fungus Verticillium dahliae, can seriously reduce the yield and quality of cotton. The complex mechanism underlying cotton resistance to Verticillium wilt remains largely unknown. In plants, reactive oxygen species (ROS) mediated by Rbohs is one of the earliest responses of plants to biotic and abiotic stresses. In our previous study, we performed a time-course phospho-proteomic analysis of roots of resistant and susceptible cotton varieties in response to V. dahliae, and found early differentially expressed protein burst oxidase homolog protein D (GhRbohD). However, the role of GhRbohD-mediated ROS in cotton defense against V. dahliae needs further investigation. In this study, we analyzed the function of GhRbohD-mediated resistance of cotton against V. dahliae in vitro and in vivo. Bioinformatics analysis showed that GhRbohD possessed the conservative structural attributes of Rbohs family, 12 members of RbohD out of 57 Rbohs in cotton. The expression of GhRbohD was significantly upregulated after V. dahliae inoculation, peaking at 6 hpi, and the phosphorylation level was also increased. A VIGS test demonstrated that ROS production, NO, H2O2 and Ca2+ contents of GhRbohD-silenced cotton plants were significantly reduced, and lignin synthesis and callose accumulation were damaged, important reasons for the impairment of GhRbohD-silenced cotton’s defense against V. dahliae. The expression levels of resistance-related genes were downregulated in GhRbohD-silenced cotton by qRT-PCR, mainly involving the lignin metabolism pathway and the jasmonic acid signaling pathway. However, overexpression of GhRbohD enhanced resistance of transgenic Arabidopsis to V. dahliae challenge. Furthermore, Y2H assays were applied to find that GhPBL9 and GhRPL12C may interact with GhRbohD. These results strongly support that GhRbohD activates ROS production to positively regulate the resistance of plants against V. dahliae.

Evolution of yield and quality of sainfoin (Onobrychis viciifolia Scop.) in the spring growth cycle

Agronomie ◽  
2003 ◽  
Vol 23 (3) ◽  
pp. 193-201 ◽  
Author(s):  
Giorgio Borreani ◽  
Pier Giorgio Peiretti ◽  
Ernesto Tabacco
Keyword(s):  
Growth Cycle ◽  
Onobrychis Viciifolia ◽  
Yield And Quality

Орошение томата F1 Донской в открытом грунте Московской области

2019 ◽  
Author(s):  
D.I. Engalychev ◽  
N.A. Engalycheva ◽  
A.M. Menshikh
Keyword(s):  
Experimental Data ◽  
Agricultural Technology ◽  
Tomato Fruits ◽  
River Floodplain ◽  
Yield And Quality ◽  
Open Ground ◽  
Alluvial Meadow ◽  
Standard Production ◽  
Moscow River

Представлены экспериментальные данные о влиянии капельного орошения на урожайность и качество плодов томата при выращивании культуры в открытом грунте Московской области. На плодородных аллювиальных луговых почвах Москворецкой поймы при соблюдении агротехники без орошения в среднем за три года исследований в полевых условиях получена урожайность томата F1 Донской 31,9 т/га, с орошением 48,5 т/га, в т.ч. стандартной продукции 42,6 т/га.The article presents experimental data on the effect of drip irrigation on the yield and quality of tomato fruits when growing crops in open ground of the Moscow Region. On fertile alluvial meadow soils of the Moscow river floodplain, with the observance of agricultural technology without irrigation, the field yield of tomato hybrid F1 Donskoi on average for three years of research was 31.9 t/ha, with irrigation 48.5 t/ha, incl. standard production 42.6 t/ha.

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