Prevalence of Mycotoxins and Endotoxins in Total Mixed Rations and Different Types of Ensiled Forages for Dairy Cows in Lithuania

In this study, 119 samples of total mixed rations and different types of ensiled forage (maize and grass silage, and haylage) collected in 2019–2020 from dairy farms in Lithuania were analyzed to evaluate the quantitative occurrence of mycotoxins and endotoxins. Samples were analyzed using high-performance liquid chromatography (HPLC) with a fluorescent (FLD) and an ultraviolet detector (UV) of mycotoxins and a detection assay based on the ELISA technology for endotoxins. The study included toxins regulated within the European Union (aflatoxin B1 (AFB1), zearalenone (ZEA), deoxynivalenol (DON) and T-2 toxin) and nonregulated toxins (endotoxins). Mycotoxin analysis showed that 49.58% of the samples out of 119 were positive for AFB1, 52.11% for ZEA and DON, 55.47% for T-2 toxin and 84.04% for endotoxins. In the contaminated samples, the highest mean values of AFB1 and T-2 toxin were determined in the grass silage samples, while ZEA and DON–were determined in the maize silage samples. Maize silage samples had the highest ZEA and DON concentrations, exceeding the EU maximum permissible concentration limits. In the haylage samples, AFB1 mycotoxin exceeded the maximum concentration limits. The highest mean value of endotoxins was determined in the total mixed rations samples. This is the first study to provide information about the concentrations of mycotoxins and endotoxins in total mixed rations and different types of ensiled forages for dairy cows in Lithuania.

Long-Term Effects of the Use of Organic Amendments and Crop Rotation on Soil Properties in Southeast Spain

The evolution of soil chemical properties over 20 years was monitored to assess the effects of the change in soil management from a rainfed to an irrigated model and the use of organic amendments and crop rotation. Intensive agriculture has been the activity that has caused most degradation and contamination of this soil. Long-term monitoring of the soil profile made it possible to assess its response to the application of sustainable agricultural techniques intended to offset these effects. Three profiles of the same soil were studied—P1 (1998), P2 (2003), P3 (2017)—to show the evolution in time and space. An incipient degradation process was detected in the first five years, verified by increases in salinity (2.3 dS m−1), exchangeable Na (0.5 g kg−1), and TN (1.3 g kg−1) in P2 in comparison with P1 (1.0, 0.2, and 1.1, respectively). There was also leaching towards the deep horizons for TN (0.4, 0.9, and 0.7 g kg−1 for P1, P2, and P3, respectively), and for assimilable elements such as P (1.1, 6.4, and 3.8), Fe (2.0, 2.1, and 5.6), Mn (0.3, 6.5, and 1.9), Zn (0.3, 0.5, and 0.9), and Cu (0.5, 0.6, and 1.3) (all mg kg−1, for P1, P2, and P3, respectively). Between 2004 and 2017, organic amendments (sheep manure) were reduced by 50%, crop rotation was intensified, and green fertilization and forage maize cultivation were included. As a result, P3 showed an improvement in comparison with P2, with decreases in EC (1.4 dS m−1), exchangeable Na (0.2 g kg−1), and TN (0.8 g kg−1). The change in soil management enhanced some soil functions (carbon sink and chemical fertility) and attenuated soil degradation.

Effect of Integrated Potassium Application on Growth, Yield and Micronutrient Uptake by Forage Maize (Zea mays L.)

A pot experiment was conducted during kharif season of 2019 to carry out the study on “Interactive effect of potash (K2O), potassium mobilizing bacteria (KMB) and FYM on forage yield, nutrient uptake by forage maize and soil fertility in a loamy sand soil of middle Gujarat”. Application of K2O @ 60 kg ha-1, KMB and FYM recorded significantly the highest plant height of forage maize at harvest over respective control. Crop fertilized with K2O @ 60 kg ha-1 and KMB gave significantly the highest green forage and dry matter yield. The results indicated that application of K2O @ 60 kg ha-1, potassium mobilizing bacteria recorded significantly the highest uptake of N, P, K, Fe and Zn by crop at harvest. Significantly the highest uptake of N, K and Cu were found with application of FYM @10 t ha-1. Significantly the highest K uptake by maize as well as higher P and Zn uptake by maize were observed due to interaction effect of K × KMB (60 kg K2O ha-1 with KMB). In case of N and Cu uptake by maize were noted the Significantly higher due to interaction effect of K × KMB (30 kg K2O ha-1 with KMB) and K × KMB × FYM (60 kg K2O ha-1 with KMB and FYM), respectively. The integrated use of potassium fertilizers along with KBM or in combination with FYM significantly improved the maize grain and nutrient uptake.

Precision Nitrogen Management for Enhancing Yield and Quality of Fodder Maize

A field experiment was conducted during Kharif 2020 at Dry land Agriculture Research Station, Rangreth, Srinagar, Kashmir on Precision Nitrogen Management for enhancing fodder yield and nitrogen use efficiency in forage maize variety SFM-1 (KDFM-1) planted in a spacing of 30 x10 cm. The treatments consisted of T1 (No N), T2 50 kg N/ha (40% N basal) + remaining based on SPAD meter critical value of 40, T3 50 kg N/ha (40% N basal) + remaining based on SPAD meter critical value of 50, T4 50 kg N/ha (40% N basal) + remaining based on LCC 4, T5 50 kg N/ha (40% N basal) + remaining based on LCC 5, T6 100 kg N/ha (40% N basal) + remaining based on SPAD meter critical value of 40, T7 100 kg N/ha (40% N basal) + remaining based on SPAD meter critical value of 50, T8 100 kg N/ha (40% N basal) + remaining based on LCC 4, T9 100 kg N/ha (40% N basal) + remaining based on LCC 5, T10 150 kg N/ha (40% N basal) + remaining based on SPAD meter critical value of 40, T11 150 kg N/ha (40% N basal) + remaining based on SPAD meter critical value of 50, T12 150 kg N/ha (40% N basal) + remaining based on LCC 4, T13150 kg N/ha (40% N basal) + remaining based on LCC 5, T14 As per recommended package of practices (50% N as basal, remaining 50% at 30 days after sowing).The treatments were replicated thrice in a randomized block design. The results recorded during the year indicated that production of fodder maize was better with the treatment T13 (150 kg N/ha (40% N basal) + remaining based on LCC 5. It recorded 470.01 q green and 135.02 q dry matter yield per hectare. The growth parameters namely; plant height; number of leaves per plant and quality were also improved with this treatment as compared to other treatments.

Quantification of the Impact of Temperature, CO2, and Rainfall Changes on Swedish Annual Crops Production Using the APSIM Model

Ongoing climate change is already affecting crop production patterns worldwide. Our aim was to investigate how increasing temperature and CO2 as well as changes in precipitation could affect potential yields for different historical pedoclimatic conditions at high latitudes (i.e., >55°). The APSIM crop model was used to simulate the productivity of four annual crops (barley, forage maize, oats, and spring wheat) over five sites in Sweden ranging between 55 and 64°N. A first set of simulations was run using site-specific daily weather data acquired between 1980 and 2005. A second set of simulations was then run using incremental changes in precipitation, temperature and CO2 levels, corresponding to a range of potential future climate scenarios. All simulation sets were compared in terms of production and risk of failure. Projected future trends showed that barley and oats will reach a maximum increase in yield with a 1°C increase in temperature compared to the 1980–2005 baseline. The optimum temperature for spring wheat was similar, except at the northernmost site (63.8°N), where the highest yield was obtained with a 4°C increase in temperature. Forage maize showed best performances for temperature increases of 2–3°C in all locations, except for the northernmost site, where the highest simulated yield was reached with a 5°C increase. Changes in temperatures and CO2 were the main factors explaining the changes in productivity, with ~89% of variance explained, whereas changes in precipitation explained ~11%. At the northernmost site, forage maize, oats and spring wheat showed decreasing risk of crop failure with increasing temperatures. The results of this modeling exercise suggest that the cultivation of annual crops in Sweden should, to some degree, benefit from the expected increase of temperature in the coming decades, provided that little to no water stress affects their growth and development. These results might be relevant to agriculture studies in regions of similar latitudes, especially the Nordic countries, and support the general assumption that climate change should have a positive impact on crop production at high latitudes.