scholarly journals Detection and Evaluation of Serological Biomarkers to Predict Osteoarthritis in Anterior Cruciate Ligament Transection Combined Medial Meniscectomy Rat Model

2021 ◽  
Vol 22 (19) ◽  
pp. 10179
Author(s):  
Nian-Cih Huang ◽  
Tsorng-Shyang Yang ◽  
Prabhakar Busa ◽  
Ching-Ling Lin ◽  
Ya-Chieh Fang ◽  
...  
Keyword(s):  
Anterior Cruciate Ligament ◽  
Complete Blood Count ◽  
Present Report ◽  
Cruciate Ligament ◽  
Preclinical Model ◽  
Biochemical Profile ◽  
Anterior Cruciate Ligament Transection ◽  
Medial Meniscectomy ◽  
Anterior Cruciate ◽  
Serological Biomarkers

Biomarkers are essential tools in osteoarthritis (OA) research, clinical trials, and drug development. Detecting and evaluating biomarkers in OA research can open new avenues for researching and developing new therapeutics. In the present report, we have explored the serological detection of various osteoarthritis-related biomarkers in the preclinical model of OA. In this surgical OA model, we disrupted the medial tibial cartilage’s integrity via anterior cruciate ligament transection combined with medial meniscectomy (ACLT+MMx) of a single joint of Wistar rats. The progression of OA was verified, as shown by the microscopic deterioration of cartilage and the increasing cartilage degeneration scoring from 4 to 12 weeks postsurgery. The concentration of serological biomarkers was measured at two timepoints, along with the complete blood count and bone electrolytes, with biochemical analysis further conducted. The panel evaluated inflammatory biomarkers, bone/cartilage biomarkers, and lipid metabolic pathway biomarkers. In chronic OA rats, we found a significant reduction of total vitamin D3 and C-telopeptide fragments of type II (CTX-II) levels in the serum as compared to sham-operated rats. In contrast, the serological levels of adiponectin, leptin, and matrix metallopeptidase (MMP3) were significantly enhanced in chronic OA rats. The inflammatory markers, blood cell composition, and biochemical profile remained unchanged after surgery. In conclusion, we found that a preclinical model of single-joint OA with significant deterioration of the cartilage can lead to serological changes to the cartilage and metabolic-related biomarkers without alteration of the systemic blood and biochemical profile. Thus, this biomarker profile provides a new tool for diagnostic/therapeutic assessment in OA scientific research.

scholarly journals Effect of Fucoidan on Anterior Cruciate Ligament Transection and Medial Meniscectomy Induced Osteoarthritis in High-Fat Diet-Induced Obese Rats

2018 ◽  
Author(s):  
Sabri Sudirman ◽  
Alan Darmasaputra Ong ◽  
Heng-Wei Chang ◽  
Zwe-Ling Kong
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cholesterol Level ◽  
High Fat Diet ◽  
Cruciate Ligament ◽  
Weight Bearing ◽  
Hdl Cholesterol ◽  
High Fat ◽  
Anterior Cruciate Ligament Transection ◽  
Medial Meniscectomy ◽  
Anterior Cruciate

Osteoarthritis (OA) has become one of the most common disabilities among elders, especially in female. Obesity and mechanical injury causing OA are attributed to joint loading, cartilage disintegration, bone loss and inflammation as well. Several strategies used for treatment OA including non-pharmacological and pharmacological. Fucoidan possesses several bioactivities such as antitumor, antiviral, anticoagulation, anti-obesity, and immunomodulation. This study aims to investigate the effect of fucoidan in surgery-induced OA on diet-induced obesity rats. OA was induced by anterior cruciate ligament transection and partial medial meniscectomy (ACLT+MMx). Male SD rats were fed high-fat diet (HFD) for 4 weeks to induce obesity before ACLT+MMx to induce OA. OA rats were administered with intragastric water or fucoidan in three different concentrations (32 mg/kg, 64 mg/kg, and 320 mg/kg) after the surgeries for 40 days with HFD. We observed that the swelling in knee joint was alleviated and hind paw weight distribution was rectified after feeding fucoidan, with no significant effect on weight gain and feed intake. Fucoidan administration indicated no significant variation on HDL-Cholesterol level, but reduced plasma triglycerides and LDL-Cholesterol level. In addition, weight-bearing tests showed improvement in the fucoidan-treated group. Our results suggested that fucoidan may improve meniscal/ligamentous injury and obesity-induced OA.

scholarly journals Effect of Fucoidan on Anterior Cruciate Ligament Transection and Medial Meniscectomy Induced Osteoarthritis in High-Fat Diet-Induced Obese Rats

2018 ◽  
Author(s):  
Sabri Sudirman ◽  
Alan Darmasaputra Ong ◽  
Heng-Wei Chang ◽  
Zwe-Ling Kong
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cholesterol Level ◽  
High Fat Diet ◽  
Cruciate Ligament ◽  
Weight Bearing ◽  
Hdl Cholesterol ◽  
High Fat ◽  
Anterior Cruciate Ligament Transection ◽  
Medial Meniscectomy ◽  
Anterior Cruciate

Osteoarthritis (OA) has become one of the most common disabilities among elders, especially in female. Obesity and mechanical injury causing OA are attributed to joint loading, cartilage disintegration, bone loss and inflammation as well. Several strategies used for treatment OA including non-pharmacological and pharmacological. Fucoidan possesses several bioactivities such as antitumor, antiviral, anticoagulation, anti-obesity, and immunomodulation. This study aims to investigate the effect of fucoidan in surgery-induced OA on diet-induced obesity rats. OA was induced by anterior cruciate ligament transection and partial medial meniscectomy (ACLT+MMx). Male SD rats were fed high-fat diet (HFD) for 4 weeks to induce obesity before ACLT+MMx to induce OA. OA rats were administered with intragastric water or fucoidan in three different concentrations (32 mg/kg, 64 mg/kg, and 320 mg/kg) after the surgeries for 40 days with HFD. We observed that the swelling in knee joint was alleviated and hind paw weight distribution was rectified after feeding fucoidan, with no significant effect on weight gain and feed intake. Fucoidan administration indicated no significant variation on HDL-Cholesterol level, but reduced plasma triglycerides and LDL-Cholesterol level. In addition, weight-bearing tests showed improvement in the fucoidan-treated group. Our results suggested that fucoidan may improve meniscal/ligamentous injury and obesity-induced OA.

scholarly journals Effect of Fucoidan on Anterior Cruciate Ligament Transection and Medial Meniscectomy Induced Osteoarthritis in High-Fat Diet-Induced Obese Rats

2018 ◽  
Author(s):  
Sabri Sudirman ◽  
Alan Darmasaputra Ong ◽  
Heng-Wei Chang ◽  
Zwe-Ling Kong
Keyword(s):  
Anterior Cruciate Ligament ◽  
Cholesterol Level ◽  
High Fat Diet ◽  
Cruciate Ligament ◽  
Weight Bearing ◽  
Hdl Cholesterol ◽  
High Fat ◽  
Anterior Cruciate Ligament Transection ◽  
Medial Meniscectomy ◽  
Anterior Cruciate

Osteoarthritis (OA) has become one of the most common disabilities among elders, especially in female. Obesity and mechanical injury causing OA are attributed to joint loading, cartilage disintegration, bone loss and inflammation as well. Several strategies used for treatment OA including non-pharmacological and pharmacological. Fucoidan possesses several bioactivities such as antitumor, antiviral, anticoagulation, anti-obesity, and immunomodulation. This study aims to investigate the effect of fucoidan in surgery-induced OA on diet-induced obesity rats. OA was induced by anterior cruciate ligament transection and partial medial meniscectomy (ACLT+MMx). Male SD rats were fed high-fat diet (HFD) for 4 weeks to induce obesity before ACLT+MMx to induce OA. OA rats were administered with intragastric water or fucoidan in three different concentrations (32 mg/kg, 64 mg/kg, and 320 mg/kg) after the surgeries for 40 days with HFD. We observed that the swelling in knee joint was alleviated and hind paw weight distribution was rectified after feeding fucoidan, with no significant effect on weight gain and feed intake. Fucoidan administration indicated no significant variation on HDL-Cholesterol level, but reduced plasma triglycerides and LDL-Cholesterol level. In addition, weight-bearing tests showed improvement in the fucoidan-treated group. Our results suggested that fucoidan may improve meniscal/ligamentous injury and obesity-induced OA.

scholarly journals High-resolution MRI and micro-CT in an ex vivo rabbit anterior cruciate ligament transection model of osteoarthritis

2004 ◽  
Vol 12 (8) ◽  
pp. 614-626 ◽  
Author(s):  
Danika L. Batiste ◽  
Alexandra Kirkley ◽  
Sheila Laverty ◽  
Lisa M.F. Thain ◽  
Alison R. Spouge ◽  
...  
Keyword(s):  
Anterior Cruciate Ligament ◽  
High Resolution ◽  
Ex Vivo ◽  
Cruciate Ligament ◽  
Micro Ct ◽  
Anterior Cruciate Ligament Transection ◽  
High Resolution Mri ◽  
Anterior Cruciate
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