scholarly journals Electron Spin Resonance Evaluation of Buccal Membrane Fluidity Alterations by Sodium Caprylate and L-Menthol

2021 ◽  
Vol 22 (19) ◽  
pp. 10708
Author(s):  
Laxmi Shanthi Chede ◽  
Brett A. Wagner ◽  
Garry R. Buettner ◽  
Maureen D. Donovan
Keyword(s):  
Electron Spin Resonance ◽  
Stearic Acid ◽  
Membrane Fluidity ◽  
Electron Spin ◽  
Spin Labels ◽  
Permeation Enhancers ◽  
Head Groups ◽  
Spin Resonance ◽  
Sodium Caprylate ◽  
Membrane Fluidization

The ability of sodium caprylate and l-menthol to fluidize phospholipid bilayers composed of lipids simulating the buccal epithelium was investigated using electron spin resonance (ESR) to evaluate the action of these agents as permeation enhancers. 5-Doxyl stearic acid (5-DSA) and 16-doxyl stearic acid (16-DSA) were used as spin labels to identify alterations in membrane fluidity near the polar head groups or inner acyl regions of the lipid bilayer, respectively. The molecular motion of both 5-DSA and 16-DSA showed increased disorder near the polar and inner hydrophobic regions of the bilayer in the presence of sodium caprylate suggesting fluidization in both the regions, which contributes to its permeation enhancing effects. L-menthol decreased the order parameter for 16-DSA, showing membrane fluidization only in the inner acyl regions of the bilayer, which also corresponded to its weaker permeation enhancing effects. The rapid evaluation of changes in fluidity of the bilayer in the presence of potential permeation enhancers using ESR enables improved selection of effective permeation enhancers and enhancer combinations based on their effect on membrane fluidization.

Changes of Electron Spin Resonance Membrane Fluidity in Hexadecane-Induced Hyperproliferative Epidermis

1989 ◽  
Vol 93 (5) ◽  
pp. 682-686 ◽  
Author(s):  
Takaho Tanaka ◽  
Ryohei Ogura ◽  
Toshihiro Hidaka ◽  
Masayasu Sugiyama
Keyword(s):  
Electron Spin Resonance ◽  
Membrane Fluidity ◽  
Electron Spin ◽  
Spin Resonance

scholarly journals High-field electron spin resonance of spin labels in membranes

2002 ◽  
Vol 116 (1-2) ◽  
pp. 93-114 ◽  
Author(s):  
Derek Marsh ◽  
Dieter Kurad ◽  
Vsevolod A. Livshits
Keyword(s):  
Electron Spin Resonance ◽  
Electron Spin ◽  
High Field ◽  
Spin Labels ◽  
Field Electron ◽  
Spin Resonance

Electron spin resonance studies of fatty acid-induced alterations in membrane fluidity in cultured endothelial cells

1995 ◽  
Vol 27 (7) ◽  
pp. 665-673 ◽  
Author(s):  
Angelina Alvarado Cader ◽  
D. Allan Butterfield ◽  
Bruce A. Watkins ◽  
Byung Hong Chung ◽  
Bernhard Hennig
Keyword(s):  
Endothelial Cells ◽  
Electron Spin Resonance ◽  
Fatty Acid ◽  
Membrane Fluidity ◽  
Electron Spin ◽  
Cultured Endothelial Cells ◽  
Spin Resonance

scholarly journals Roles of Adiponectin and Oxidative Stress in the Regulation of Membrane Microviscosity of Red Blood Cells in Hypertensive Men—An Electron Spin Resonance Study

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Kazushi Tsuda
Keyword(s):  
Oxidative Stress ◽  
Electron Spin Resonance ◽  
Membrane Fluidity ◽  
Electron Spin ◽  
Order Parameter ◽  
Resonance Method ◽  
Electron Spin Resonance Study ◽  
Plasma Adiponectin ◽  
Spin Resonance ◽  
And Oxidative Stress

This study was undertaken to investigate possible relationships among plasma adiponectin, 8-iso-prostaglandin F2α(8-iso-PG F2α: an index of oxidative stress), and membrane fluidity (a reciprocal value of microviscosity) in hypertensive and normotensive men using an electron spin resonance-method. The order parameter (S) for the spin-label agent (5-nitroxide stearate) in red blood cell (RBC) membranes was higher in hypertensive men than in normotensive men, indicating that membrane fluidity was decreased in hypertension. Plasma adiponectin and NO metabolites levels were lower in hypertensive men than in normotensive men. In contrast, plasma 8-iso-PG F2αlevels were increased in hypertensive men compared with normotensive men. Plasma adiponectin concentration was correlated with plasma NO-metabolites, and inversely correlated with plasma 8-iso-PG F2α. The order parameter (S) of RBCs was inversely correlated with plasma adiponectin and plasma NO metabolite levels, and positively correlated with plasma 8-iso-PG F2α, suggesting that the reduced membrane fluidity of RBCs might be associated with hypoadiponectinemia, endothelial dysfunction, and increased oxidative stress. In a multivariate regression analysis, adiponectin and 8-iso-PG F2αwere significant determinants of membrane fluidity of RBCs after adjustment for general risk factors. These results suggest that adiponectin and oxidative stress might have a close correlation with rheologic behavior and microcirculation in hypertension.

scholarly journals Lipid analyses and fluidity studies by electron spin resonance of red cell membranes in hereditary high red cell membrane phosphatidylcholine hemolytic anemia

Blood ◽  
1984 ◽  
Vol 64 (5) ◽  
pp. 1129-1134 ◽  
Author(s):  
Y Yawata ◽  
T Sugihara ◽  
M Mori ◽  
S Nakashima ◽  
Y Nozawa
Keyword(s):  
Electron Spin Resonance ◽  
Cell Membrane ◽  
Membrane Fluidity ◽  
Hemolytic Anemia ◽  
Electron Spin ◽  
Order Parameter ◽  
Red Cells ◽  
Red Cell ◽  
Red Cell Membrane ◽  
Spin Resonance

Abstract Membrane lipid analyses and electron spin resonance (ESR) studies of membrane fluidity were carried out on the red cells of a Japanese patient with hereditary high red cell membrane phosphatidylcholine hemolytic anemia (HPCHA). Increased amounts of phosphatidylcholine (PC) and cholesterol were found in the membrane lipids of the affected patient, despite normal plasma lipids. The order parameter of cholesterol-free pure phospholipid liposomes prepared from this patient's red cells was decreased, apparently because of the increased PC. In contrast, the order parameter of the total red cell lipid liposomes (containing free cholesterol) was essentially normal. The overall fluidity of the intact red cells was determined by ESR with a spin probe, 5-SAL. Again, the order parameters were normal in the intact red cells of the patient with HPCHA. This suggests that the concomitant increase of membrane cholesterol and phosphatidylcholine serves to maintain normal membrane fluidity in the HPCHA red cells.

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