scholarly journals Effects of Training Status and Exercise Mode on Global Gene Expression in Skeletal Muscle

2021 ◽  
Vol 22 (22) ◽  
pp. 12578
Author(s):  
Daniel A. Bizjak ◽  
Martina Zügel ◽  
Gunnar Treff ◽  
Kay Winkert ◽  
Achim Jerg ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Expression Profiles ◽  
Strength Exercise ◽  
Training Status ◽  
Transcriptional Responses ◽  
Exercise Mode ◽  
Muscle Gene Expression ◽  
Pathway Analyses ◽  
Muscle Gene

The aim of this study was to investigate differences in skeletal muscle gene expression of highly trained endurance and strength athletes in comparison to untrained individuals at rest and in response to either an acute bout of endurance or strength exercise. Endurance (ET, n = 8, ⩒O2max 67 ± 9 mL/kg/min) and strength athletes (ST, n = 8, 5.8 ± 3.0 training years) as well as untrained controls (E-UT and S-UT, each n = 8) performed an acute endurance or strength exercise test. One day before testing (Pre), 30 min (30′Post) and 3 h (180′Post) afterwards, a skeletal muscle biopsy was obtained from the m. vastus lateralis. Skeletal muscle mRNA was isolated and analyzed by Affymetrix-microarray technology. Pathway analyses were performed to evaluate the effects of training status (trained vs. untrained) and exercise mode-specific (ET vs. ST) transcriptional responses. Differences in global skeletal muscle gene expression between trained and untrained were smaller compared to differences in exercise mode. Maximum differences between ET and ST were found between Pre and 180′Post. Pathway analyses showed increased expression of exercise-related genes, such as nuclear transcription factors (NR4A family), metabolism and vascularization (PGC1-α and VEGF-A), and muscle growth/structure (myostatin, IRS1/2 and HIF1-α. The most upregulated genes in response to acute endurance or strength exercise were the NR4A genes (NR4A1, NR4A2, NR4A3). The mode of acute exercise had a significant effect on transcriptional regulation Pre vs. 180′Post. In contrast, the effect of training status on human skeletal muscle gene expression profiles was negligible compared to strength or endurance specialization. The highest variability in gene expression, especially for the NR4A-family, was observed in trained individuals at 180′Post. Assessment of these receptors might be suitable to obtain a deeper understanding of skeletal muscle adaptive processes to develop optimized training strategies.

scholarly journals Diet Composition Impacts Skeletal Muscle Gene Expression Profiles of Young Adult and Geriatric Dogs

2006 ◽  
Vol 20 (4) ◽  
Author(s):  
Lisa Kathleen Karr‐Lilienthal ◽  
Carolyn Josephine Apanavicius ◽  
Kelly Scott Swanson
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Young Adult ◽  
Diet Composition ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Muscle Gene Expression ◽  
Muscle Gene

Skeletal muscle gene expression profiles in 20–29 year old and 65–71 year old women

2004 ◽  
Vol 39 (3) ◽  
pp. 369-377 ◽  
Author(s):  
Stephen Welle ◽  
Andrew I Brooks ◽  
Joseph M Delehanty ◽  
Nancy Needler ◽  
Kirti Bhatt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Muscle Gene Expression ◽  
Muscle Gene

Skeletal muscle gene expression in space‐flown rats

2004 ◽  
Vol 18 (3) ◽  
pp. 522-524 ◽  
Author(s):  
Takeshi Nikawa ◽  
Kazumi Ishidoh ◽  
Katsuya Hirasaka ◽  
Ibuki Ishihara ◽  
Madoka Ikemoto ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Muscle Gene Expression ◽  
Muscle Gene

Effects of equine myostatin (MSTN) genotype variation on transcriptional responses in Thoroughbred skeletal muscle

2019 ◽  
Vol 15 (5) ◽  
pp. 327-338
Author(s):  
K. Bryan ◽  
L.M. Katz ◽  
E.W. Hill
Keyword(s):  
Skeletal Muscle ◽  
Skeletal Muscle Fibre ◽  
P Value ◽  
Transcriptional Responses ◽  
Myostatin Gene ◽  
Muscle Gene Expression ◽  
Gene Differential Expression ◽  
Sine Insertion ◽  
Significant Enrichment ◽  
Muscle Gene

Myostatin gene (MSTN) variation influences distance aptitude in Thoroughbreds as a consequence of functional physiological effects including skeletal muscle fibre type and muscle hypertrophy variation. A promotor region short interspersed nuclear element (SINE) insertion, tagged by SNP g.66493737-C, alters MSTN mRNA expression. We tested the hypothesis that skeletal muscle gene expression varies among MSTN genotypes due to differential up- or down-stream gene signalling pathways that may be influenced by exercise and training and consequently contribute to variation in exercise phenotypes. Skeletal muscle biopsies were collected from Thoroughbreds previously genotyped for MSTN (n=35 CC, n=50 CT, n=9 TT) at three different time-points: untrained at rest (UR), untrained after exercise (UE) and trained at rest (TR). Gene differential expression (DE) was determined from RNAseq data using DESeq2 (Benjamini-Hochberg P-value <0.05). Functional over-representation analysis was performed in DAVID. In UR samples, one, nine and 47 genes were DE between CC vs CT, CT vs TT and C:C vs TT, respectively. The OSGEPL1 gene, located <250 Kb proximal to MSTN, was DE among all cohorts. Six genes were DE in UE between CC vs TT including OSGEPL1, FGF10 and COQ8A. There was significant enrichment for GO categories related to mitochondria in TR. Comparison of the exercise response (UR vs UE) revealed patterns of expression that were opposing; i.e. CHRNG was 0.857 log2FC in the TT cohort but 2.055 log2FC in the CC cohort. Genes located in proximity to MSTN and involved in mitochondrial function were most significantly different among genotype cohorts. Patterns of DE among genotypes suggests gene-regulated influence on the phenotype. Understanding these patterns may assist genotype-guided training strategies.

Effects of ractopamine and sex on serum metabolites and skeletal muscle gene expression in finishing steers and heifers

2010 ◽  
Vol 88 (4) ◽  
pp. 1349-1357 ◽  
Author(s):  
D. K. Walker ◽  
E. C. Titgemeyer ◽  
T. J. Baxa ◽  
K. Y. Chung ◽  
D. E. Johnson ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Serum Metabolites ◽  
Muscle Gene Expression ◽  
Finishing Steers ◽  
Muscle Gene

scholarly journals Skeletal muscle in aged mice reveals extensive transformation of muscle gene expression

BMC Genetics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
I-Hsuan Lin ◽  
Junn-Liang Chang ◽  
Kate Hua ◽  
Wan-Chen Huang ◽  
Ming-Ta Hsu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Aged Mice ◽  
Muscle Gene Expression ◽  
Muscle Gene

Acute endurance exercise stimulates circulating levels of mitochondrial derived peptides in humans

2021 ◽  
Author(s):  
Ferdinand von Walden ◽  
Rodrigo Fernandez-Gonzalo ◽  
Jessica Maria Norrbom ◽  
Eric B. Emanuelsson ◽  
Vandre C. Figueiredo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Diabetes Type 2 ◽  
Knee Extension ◽  
12S Rrna ◽  
Mt Dna ◽  
Reading Frame ◽  
Muscle Gene Expression ◽  
Muscle Gene ◽  
Circulating Levels

Mitochondrial derived peptides (MDPs) humanin (HN) and mitochondrial open reading frame of the 12S rRNA-c (MOTS-c) are involved in cell survival, suppression of apoptosis and metabolism. Circulating levels of MDPs are altered in chronic diseases such as diabetes type 2 and chronic kidney disease. Whether acute resistance (RE) or endurance (EE) exercise modulates circulating levels of HN and MOTS-c in humans is unknown. Following familiarization, subjects were randomized to EE (n=10, 45 min cycling at 70% of estimated VO2max), RE (n=10, 4 sets x 7RM, leg press and knee extension), or control (CON, n=10). Skeletal muscle biopsies and blood samples were collected before and at 30 minutes and 3 hours following exercise. Plasma concentration of HN and MOTS-c, skeletal muscle MOTS-c as well as gene expression of exercise related genes were analyzed. Acute EE and RE promoted changes in skeletal muscle gene expression typically seen in response to each exercise modality (c-Myc, 45S pre-rRNA, PGC-1α-total and PGC-1α-ex1b). At rest, circulating levels of HN were positively correlated to MOTS-c levels and age. Plasma levels of MDPs were not correlated to fitness outcomes (VO2max, leg strength or muscle mitochondrial (mt) DNA copy number). Circulating levels of HN were significantly elevated by acute EE but not RE. MOTS-C levels showed a trend to increase after EE. These results indicate that plasma MDP levels are not related to fitness status but that acute EE increases circulating levels of MDPs, in particular HN.

scholarly journals Skeletal Muscle Gene Expression Study of Monozygous Twins with 35 Years of Divergent Exercise History

2018 ◽  
Vol 50 (5S) ◽  
pp. 115
Author(s):  
Adam Osmond ◽  
Robert J. Talmadge ◽  
Katie E. Bathgate ◽  
James R. Bagley ◽  
Lee E. Brown ◽  
...  
Keyword(s):  
Gene Expression ◽  
Skeletal Muscle ◽  
Gene Expression Study ◽  
Expression Study ◽  
Muscle Gene Expression ◽  
Muscle Gene ◽  
Monozygous Twins
Sign in / Sign up

Export Citation Format

Share Document