Gene expression signature of exercise and change of diet on non-alcoholic fatty liver disease in mice

Non-alcoholic fatty liver diseases (NAFLD) are particularly prevalent in the general Western adult population, with around one third of the population suffering from the disease. Evidence shows that NAFLD is associated with metabolic syndromes such as obesity, insulin resistance, and hypertension. Currently, the sole therapy for NAFLD involves exercise intervention. Studies showed that, with and without weight loss, exercise interventions produced a significant cutback in intrahepatic lipid content in humans, but better controlled studies that can investigate the cellular and molecular mechanisms are still lacking. In the current study we perform RNA sequencing analysis on liver samples from C57BL/6 mice submitted to aerobic exercise and diet interventions that are human-translatable and determine the genetic expression signature of exercise in the NAFLD onset. We show that aerobic exercise affects genes and pathways related to liver metabolism, muscle contraction and relaxation, immune response and inflammation, and development of liver cancer, counteracting non-alcoholic steatohepatitis and hepatocellular carcinoma development. While genes and pathways implicating immune response are activated by aerobic exercise in all interventions, the most effective intervention in terms of improvement of NASH is the combination of aerobic exercise with change of diet.

Association of blood pressure and postural control in older adults with hypertension: an observational study

The study investigated the association of orthostatic hypotension (OH) with functional position change and balance in older adults with hypertension. The presence of OH was assessed with intermittent (OH intermittent) and continuous blood pressure (OH continuous) monitoring. The change in functional position was tested with sitting to standing assessment, balance performance using activity specific balance confidence scale (ABC), and timed up and go test (TUG). Testing unilateral and bilateral standing with and without altered sensory inputs was tested using the Humac balance system. ABC, TUG, and standing up time showed no significant association with OH intermittent and OH continuous. A significant association was found between bilateral standing with eyes closed on foam surface for overall stability index and OH intermittent. Older people with hypertension may be routinely examined for OH and appropriate intervention strategies should be included for comprehensive care.

Effect of resistance training and garlic extract on insulin sensitivity/resistance and biochemical parameters in diabetic rats

Diabetes is one of the most important endocrine diseases in the world and obesity is one of the risk factors for this disease. The aim of this study was to evaluate the effect of a resistance exercise and garlic extract on insulin sensitivity/resistance and signal pathway of white adipose tissue to brown factors in diabetic rats. A total of 48 male Wistar rats weighing 180 to 250 g were divided into six groups (n=8): healthy control (C), diabetic control (D), diabetic with garlic extract at a dose of 50 mg/kg body weight (bw) (D+50), diabetic with garlic extract dose of 200 mg/kg bw (D+200), diabetic resistance training (D+Ex), and diabetic resistance training with garlic extract dose of 200 mg/kg bw (D+Ex+200). Plasma irisin levels in the D+200 and D+Ex groups, as well as the D+Ex+200 group showed a significant increase compared to the D group (P<0.001), while in the D+50 group no significant change was observed. Compared with group D, the expression of peroxisome proliferator-activated receptor-gamma coactivator (PGC)-1α gene was significantly increased in groups D+200 and D+Ex, as well as group D+Ex+200 (P<0.001). It can be said that resistance exercise with garlic extract is effective in controlling diabetes and reducing its complications. It also increases the expression of PGC-1α and uncoupling protein 1 genes in white adipose tissue and therefore has a positive effect on beta cell function by irisin.

Comparison of different types of exercise training on resting blood pressure in 30-45 years old adults: a randomised controlled trial

Hypertension is the most metastasizing non-communicable disease, which has affected more than 1.4 billion people, worldwide. Exercise is the core management strategy for hypertensive individuals. Extensive literature is available for each kind of exercise that is isometric hand grip training (IHGT), resistance training (RT), and aerobic training (AT). However, the comparison between these exercise regimens has not been done in a single trial. The trial was conducted on 126 subjects in Hisar, an urban area of Haryana (INDIA), from July 2017 to December 2018. Subjects were randomly assigned to four groups: control (n=33), IHGT (n=32), RT (n=31), and AT (n=30). Study variables were measured at baseline, 2nd week, 4th week, 6th week, and also after the 8th week of intervention. Experimental groups showed significant reduction in all the components of blood pressure in comparison to control group. The RT group showed maximum reduction in blood pressure in comparison to other regimens (systolic blood pressure: RT > IHGT > AT; diastolic blood pressure: RT > AT > IHGT). However, the difference between the groups is not statistically significant (P>0.05). The study findings reveal that different kind of exercise regimens are equally effective in lowering blood pressure among pre hypertensive and stage 1 hypertension adults.

A cross sectional survey on physical fitness, mental health and associated factors in mothers of children with special needs

Mothers are the primary caregivers of a child. While caring for a special needs child, workload of caring increases by manifold and taking care of these children increases physical & mental overload. There is a dearth of studies that have objectively evaluated mental health and fitness of this group. To study status of physical fitness, mental health and associated factors in mothers of special needs children a cross-sectional survey done on 100 mothers of special needs children from special schools in the Gurugram District, Haryana, India. The Harvard Step test was used to evaluate cardiac fitness. Standard tests were used to evaluate different components of musculoskeletal fitness. Depression was assessed by Beck Depression Inventory. Descriptive statistics were used for data analysis. The Karl Pearson correlation test was used to identify correlations between mental health and fitness components and sociodemographic characteristics. Mean age and body mass index of mothers was 36.15±2.56 years and 27.4±3.25 kg/m2, respectively. 50% of the subjects were overweight, 89% scored poor in cardiopulmonary fitness, 63% had very poor muscle endurance, and 69% had average muscular flexibility. 81% of the mothers had symptoms of clinical depression. Inverse and significant correlation was present between depression and cardiorespiratory fitness (r=-0.197), strength (r=-0.242), and endurance (r=0.209). Income, total number of children and duration of care giving were inversely correlated with depression. Positive correlation was found between type of disability and depression. There was an overall decrement in cardiorespiratory and musculoskeletal fitness. Mental health was found to be poor with a large percentage of participants showing symptoms of depression.

Pilot-study of ridden walk on the circle – effects of progressive collection and lateral exercises

When collecting the horse, the rider influences stride length, forehand/hindquarters balance, and head-neck position. The study aim was to describe the vertical excursion of the withers and croup, and the sagittal cannon angles during collection and lateral exercises. Ten horses were ridden by five riders during 14 trials (1-5 per rider) on 10 m circles. Each trial included free walk, four degrees of increasing collection, and haunches-in and shoulderin. Inertial measurement units (100 Hz) were positioned on the withers, the first sacral vertebra (S1) and laterally on the cannons. Data for each exercise were stride-split. Range of motion (ROM), minima and maxima were studied in mixed models, controlling for stride duration. S1 vertical ROM ranged between 30-32 mm (highest degree of collection) and 51 mm (free walk), significantly smaller with increasing collection. S1 ROM during the inside hind limb step was smaller in haunches-in and shoulder-in compared to at the lowest degree of collection. Withers ROM ranged between 12 mm (lowest degree of collection) and 16-18 mm (highest degree of collection). Fore cannon protraction-retraction ROM ranged between 57° (highest degree of collection) and 63° (free walk). Hind cannon protraction-retraction ROM ranged between 47-50° (highest degree of collection) and 51-56° (free walk). All limbs had significantly smaller ROM at the highest degree of collection. Cannon ROMs were smaller for the outer limbs in haunches-in, and all limbs but the outer fore in shoulder-in, compared to the lowest degree of collection. Progressively decreasing ROM for fore- and hind limb cannons and S1 suggest that the riders achieved a shortening of the gait at higher degrees of collection. In shoulder-in and haunches-in, the diagonal oriented in the direction of motion showed decreased hind limb cannon ROM while forelimb cannon ROM was maintained, which could suggest increased shoulder freedom and collection of the targeted diagonal.

Exploring the key attributes of former racehorses considered to have the potential for a successful second career in horseball

The British horseracing industry is committed to safeguarding the welfare of racehorses throughout their racing careers and beyond. Former racehorses who do not retire to a stud career and are suitable for second careers are often retrained to compete in other equestrian sports. Horseball is a growing discipline, which proactively supports retraining of racehorses (RoR) that could offer a suitable second career for former racehorses. This study explored the key attributes that horseball competitors look for in a potential former racehorse to promote a successful career in the sport. Participants voluntarily completed an online, 18 question survey (SurveyMonkey®), distributed through horseball related communication channels (Facebook™, Instagram™ and the British Horseball Association). Horseball competitors were asked (a) what key physical and behavioural characteristics they considered necessary for a successful horseball horse and (b) whether they would consider competing a former racehorse. A total of 45 horseball competitors completed the survey representing a margin of error of ±13% at the 95% confidence interval for the UK horseball population (n=200). Most respondents celebrated former racehorses’ suitability for horseball competition; 73.72% (n=32) had already owned and competed a former Thoroughbred racehorse in horseball and 97.78% (n=44) would consider purchasing and competing one in the future. Former racehorses were recognised to possess key performance attributes: agility, temperament, and speed suitable for a successful second career in competitive horseball. However, injuries that impeded former racehorse performance; lameness and other chronic injuries, were deemed as detrimental to success in competitive horseball. Further work in collaboration with racehorse rehoming stakeholders is required to produce guidelines to identify specific behavioural and physical characteristics which could predict the suitability of former racehorses for successful second careers across equestrian disciplines.

Physical activities of jockeys during a working week

Horse racing and training is a physically demanding sport. The aims of this study were to quantify the physical activities of jockeys during a working week and to investigate self-reported fall and injury incidence rates of jockeys at work. A daily workload diary examining workday and physical activities was emailed to all jockeys licensed to ride in a race in New Zealand in 2020. Sixty-three jockeys (25 apprentices, 33 professional and 5 amateur riders) began the diary, representing 38% of the licensed population of jockeys in New Zealand. Jockeys worked a median of 44 (interquartile range (IQR) 33-57) hours, 6-days per week. A median of 7 (IQR 6-9) horses were ridden per day, comprising 58% of work time, with 11 (IQR 7-15) hours per week spent at training pace. Elite jockeys (high performance in the premiership table) spent more time riding in races (1.1, IQR 0.7-1.2 hours per week) than non-elite jockeys (0.0, 0.0-0.4, P=0.01), with 29% (IQR 0-54%) of their weekly rides as race rides. Extra physical training was conducted by 72% of jockeys, which consisted mainly of low intensity exercise such as pleasure riding (56%) and walking (43%). Falls during morning exercise work were recorded by 87% of respondents, 40% of which had sustained an injury in the previous 12 months. Jockeys who participated in extra physical training had higher fall incidence rates per 1000 horses ridden in morning exercise work (3.5, 95% confidence interval (CI) 3.1-3.9, P=0.002) but lower fall incidence rate in race riding (2.1, 95% CI 1.5-2.8, P<0.001) than jockeys who did no extra training (2.5, 95% CI 2.0-3.0 and 5.9, 95% CI 3.8-9.0). Elite jockeys experience a level of specific race exercise which is lacking in the other jockeys.

Efficacy of high-intensity interval training on cardiac autonomic modulation in cardiovascular diseases and lifestyle disorders: a systematic review and meta-analysis

The study aims to investigate the literature on the effect of high-intensity interval training (HIIT) on cardiac autonomic function in individuals with cardiovascular disease (CVD) and lifestyle disorders. We performed electronic database search from CENTRAL, WoS, Scopus, Pubmed, and PEDro up to 25th February 2021. Randomised control trials/quasi-experimental trials/cross-over trials that assessed the effects of HIIT with control/alternative treatment on cardiac autonomic control were included in this review. A total of 11 studies were included for qualitative analysis and among them, 8 were quantitatively analysed. A random-effect model of standardised mean difference (SMD) and mean difference of the respective outcome measures for cardiac autonomic control was determined. The findings of the qualitative analysis revealed the beneficial effects of HIIT on cardiac autonomic modulation. However, the majority of the studies had an unclear or high risk of bias for randomisation, concealment methods, and blinding of participants to the intervention that could have influenced the interpretation of the findings. The SMD revealed a significant effect of HIIT on standard deviation of N-N intervals (SDNN) (ms) [SMD: 0.40, 95% confidence interval (CI): -0.001 to 0.80, P=0.05], high frequency power (HF) (ms2) [0.46, 95% CI: 0.17 to 0.76, P=0.002], and ratio of low and high frequency power, (LF/HF) [-0.80, 95% CI: -1.27 to -0.33, P=0.0008]. In conclusion, HIIT may effectively modulate cardiac autonomic function by increasing parasympathetic dominance, sympathetic withdrawal, and sympathovagal balance in individuals with CVD and lifestyle disorders. The study has a PROSPERO registration number: CRD42021231225

Understanding rider:horse bodyweight ratio trends, weight management practices and rider weight perceptions within leisure and amateur riders in the UK

Horse riders in the UK have a legal responsibility for the welfare of the horses in their care, outlined by the Animal Welfare Act (2006). Understanding weight management factors that influence rider: horse bodyweight (RHBW) ratio is key to safeguarding horse welfare as human obesity rates increase. Recent high-profile incidents have seen riders being asked to dismount for being too heavy, demonstrating an awareness of the possible impact of excessive rider weight, threatening the equestrian industry’s social licence to operate. This study investigated RHBW trends within the UK leisure and amateur rider population to understand rider perception of ‘ideal’ RHBW and factors influencing rider and horse weight management. An online survey (SurveyMonkey®) was distributed via UK equine-related Facebook™ groups and collected information on horse and rider demographics, rider weight management strategies and respondents’ views on the importance of rider weight on horse welfare. Kruskal-Wallis analyses with Mann Whitney U post-hoc tests identified whether differences in respondent views differed between RHBW groups. A total of 971 riders completed the survey; respondents were aged between 18-65+ years old and 88% (n=953) were experienced riders. RHBWs were calculated for 764 (79%) of respondents as 21.2% (n=206) did not know either their own and/or their horses’ weight. Weight tapes (44.5%; n=432) and weigh bridges (29.5%; n=286) were common horse weight estimation methods. RHBWs ranged from 4.9% to 21.88%, mean: 12.5%±2.7%. Riders with lower RHBW thought about their own weight less and measured their horses’ weight less often than those with higher ratios (P<0.005, P<0.0004, respectively). The majority of riders who participated were weight conscious and recognised potential detrimental impacts associated with increased rider weight. Development of RHBW guidelines supported by equestrian governing bodies would highlight the need for riders to consider the impact of weight and support them in choosing suitable horses.