scholarly journals Influence of Distance from the Host on Parasitisation by Psyttalia concolor (Szépligeti) (Hymenoptera: Braconidae)

Insects ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 316
Author(s):  
Nuray Baser ◽  
Gabriella Lo Verde ◽  
Gülay Kaçar ◽  
Flutura Lamaj ◽  
Vincenzo Verrastro ◽  
...  
Keyword(s):  
Laboratory Tests ◽  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Olive Fruit Fly ◽  
Female Ratio ◽  
Ovipositor Length ◽  
Control Programmes ◽  
Psyttalia Concolor ◽  
Proper Setting

The olive fruit fly, Bactrocera oleae, is considered the main olive pest worldwide, and has been the target of biological control programmes through the release of the braconid parasitoid Psyttalia concolor. Laboratory tests were performed to evaluate the influence of distance from the host on parasitisation, placing larvae of the substitute host Ceratitis capitata at seven distances (0, 0.5, 1, 1.5, 2, 2.5, 3 mm) and four different time periods (7, 15, 30, 60 min). Moreover, field collected olives of Ogliarola Barese cultivar infested by B. oleae were exposed to P. concolor females to confirm its ability to parasitise B. oleae in small olives. Psyttalia concolor oviposition was inhibited at 2.5 and 3 mm due to the ovipositor length of the parasitoid females (2.7 mm). Hosts were easily parasitised at distances between 0 and 1.5 mm. The thin fruit pulp (up to 3.5 mm) of field collected olives allowed the parasitisation to occur also in mature fruits. At the best combination distance/time (0 mm, 30 min), tests performed with different larvae/parasitoid female ratio showed an increasing emergence of P. concolor (from 20% to 57%) with larvae/parasitoid ratio increasing from 0.11 to 0.74. The results of the present study might optimise the mass rearing of P. concolor, through a proper setting of its parameters, such as the host/parasitoid ratio, exposure distances, and interaction time.

New larval agar-based diet for laboratory rearing of Mediterranean fruit fly Ceratitis capitata (Diptera, Tephritidae)

Biologia ◽  
2007 ◽  
Vol 62 (4) ◽  
Author(s):  
Martina Pašková
Keyword(s):  
Ceratitis Capitata ◽  
Potato Starch ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Pupal Weight ◽  
Larval Population ◽  
Laboratory Rearing ◽  
Heat Treated ◽  
New Formulation ◽  
The Cost

AbstractFive larval diets for laboratory rearing of Ceratitis capitata were tested. These diets were based on wheat bran, microcellulose, potato starch and agar. To evaluate the quality of diet, pupal rearing efficacy and pupal weight were checked. The best results were obtained with an agar based diet used for Manduca sexta laboratory rearing. To simplify the preparation and to reduce the cost of this diet, a new formulation was developed. Larvae reared on the new agar-based diet achieved higher pupal rearing efficacy than larvae reared on bran diet recently used in medfly mass rearing facilities. Heat treated medflies reared on the new agar-based diet achieved similar pupal rearing efficacy with heat treated medflies reared on bran diet. When testing population density, higher pupal rearing efficacy was again achieved on new diet. The highest pupal rearing efficacy was achieved with 100 eggs per 25 g of diet, lowest with 500 eggs per 25 g of diet. Concerning pupal weight, there was no difference in results achieved on Petri dishes with different larval population densities. Larvae reared on new agar-based diet reached better results than larvae reared on bran diet. The preparation of the new diet is simple and the cost is low, so it is good for laboratory tests and rearing.

Comparison of two laboratory cultures of Psyttalia concolor (Hymenoptera: Braconidae), as a parasitoid of the olive fruit fly

2006 ◽  
Vol 39 (2) ◽  
pp. 248-255 ◽  
Author(s):  
Karen R. Sime ◽  
Kent M. Daane ◽  
Russell H. Messing ◽  
Marshall W. Johnson
Keyword(s):  
Fruit Fly ◽  
Olive Fruit Fly ◽  
Olive Fruit ◽  
Laboratory Cultures ◽  
Psyttalia Concolor

Comparative study of the eggshell of the fruit flies Dacus oleae and Ceratitis capitata (Diptera: Trypetidae)

1985 ◽  
Vol 63 (9) ◽  
pp. 2194-2206 ◽  
Author(s):  
Lukas H. Margaritis
Keyword(s):  
Biochemical Analysis ◽  
Ceratitis Capitata ◽  
Light And Electron Microscopy ◽  
Fruit Fly ◽  
Fruit Flies ◽  
Olive Fruit Fly ◽  
Dacus Oleae ◽  
Structural Functions ◽  
Micropylar Canal ◽  
Eggshell Proteins

The eggshell of two fruit flies of economic importance has been studied by means of light and electron microscopy. The eggshell of the olive fruit fly, Dacus oleae, contains compact endochorion, whereas that of the Mediterranean fruit fly, Ceratitis capitata, exhibits a holey endochorionic layer. The eggshells of both species have an innermost chorionic layer which appears to be crystalline in substructure. The anterior pole in each case contains the micropylar canal but in addition it bears an elaborate "cup" in Dacus oleae. Both species exhibit peroxidase activity in most eggshell layers and, as in the case of Drosophila melanogaster, it is thought that the enzyme causes covalent cross-linking of the eggshell proteins producing a resilin-type configuration with rubberlike properties. The above features are related to specific structural functions of the eggshell during fertilization, oviposition, and respiration of the embryo. Biochemical analysis has revealed that the two species have very few eggshell proteins that are similar in molecular weight.

scholarly journals Effect of exposure time on mass-rearing production of the olive fruit fly parasitoid, Psyttalia lounsburyi (Hymenoptera: Braconidae)

2017 ◽  
Vol 142 (3) ◽  
pp. 319-326 ◽  
Author(s):  
M. La-Spina ◽  
C. Pickett ◽  
K. M. Daane ◽  
K. Hoelmer ◽  
A. Blanchet ◽  
...  
Keyword(s):  
Exposure Time ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Olive Fruit Fly ◽  
Olive Fruit

Laboratory tests of insect growth regulators as bait sprays for the control of the olive fruit fly, Bactrocera oleae (Diptera: Tephritidae)

2012 ◽  
Vol 69 (4) ◽  
pp. 520-526 ◽  
Author(s):  
Ismael Sánchez-Ramos ◽  
Cristina E Fernández ◽  
Manuel González-Núñez ◽  
Susana Pascual
Keyword(s):  
Growth Regulators ◽  
Laboratory Tests ◽  
Fruit Fly ◽  
Bactrocera Oleae ◽  
Olive Fruit Fly ◽  
Insect Growth Regulators ◽  
Olive Fruit ◽  
Insect Growth

Genetic and cytogenetic analysis of Y-autosome translocations in the Mediterranean fruit fly, Ceratitis capitata

Genome ◽  
1992 ◽  
Vol 35 (2) ◽  
pp. 264-272 ◽  
Author(s):  
Ph. Kerremans ◽  
E. Gencheva ◽  
G. Franz
Keyword(s):  
Ceratitis Capitata ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Mediterranean Fruit Fly ◽  
Structural Basis ◽  
Mitotic Chromosomes ◽  
Genetic Sexing ◽  
Radiation Induced ◽  
Low Levels ◽  
The Mediterranean

Radiation-induced translocations in the Mediterranean fruit fly, Ceratitis capitata, linking the Y chromosome to either autosome 3 or 4 produced pseudolinkage between sex and the mutations dark pupa (dp) and apricot eye (ap), respectively. The genetic behaviour of six new strains is described and the structural basis of five of them is determined through analysis of polytene and mitotic chromosomes. Five strains exhibited low levels of recombination; however, one strain produced a larger number than expected of aberrant, wild-type females. We provide evidence that this is the consequence of the survival of adjacent-1 segregation products until adulthood.Key words: medfly, mass rearing, genetic sexing, recombination, segregation.

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