Physicochemical Properties, Cytocompatibility and Antibiofilm Activity of a New Calcium Silicate Sealer

The aim of this study was to evaluate the physicochemical properties, cytocompatibility and antibiofilm activity of a new calcium silicate-based endodontic sealer, Sealer Plus BC (MK Life, Brazil), in comparison with TotalFill BC Sealer (FKG Dentaire SA, Switzerland) and AH Plus (Dentsply, Germany). Setting time and flow were evaluated based on ISO 6876 standard. The pH was evaluated after different periods, and radiopacity by radiographic analysis (mmAl). Solubility (% mass loss) and volumetric change (by micro-CT) were assessed after 30 days of immersion in distilled water. Cytocompatibility was assessed by methyltetrazolium (MTT) and neutral red (NR) assays, after exposure of Saos-2 cells to the sealer extract for 24 h. An additional analysis was performed by using MTT assay after 1, 3 and 7 days of exposure of Saos-2 to the sealers 1:8 dilution extracts. Antibiofilm activity against Enterococcus faecalis and/or Candida albicans was evaluated by crystal violet assay and modified direct contact test. The physicochemical properties were analyzed using ANOVA/Tukey tests; MTT and NR data were analyzed by ANOVA and Bonferroni tests; the antimicrobial tests were analyzed by Kruskal-Wallis and Dunn tests (α=0.05). Sealer Plus BC had proper setting time, radiopacity, flow and alkalization capacity. Sealer Plus BC was significantly more soluble than AH Plus (p<0.05) and presented volumetric change similar to AH Plus and TotalFill BC (p>0.05). Sealer Plus BC presented antibiofilm activity and no cytotoxic effect. In conclusion, although Sealer Plus BC had higher solubility, this sealer showed proper physicochemical properties, cytocompatibility, and antibiofilm activity.

Applications of PERALS-Alpha Spectrometry for the Investigation of Radionuclides in Water Samples

In this paper, experiences of the last 20 years with the PERALS-technique are described. PERALS stands for photo electron-rejecting alpha liquid scintillation. This liquid scintillation technique was developed by Jack McDowell in the 1970s and is a powerful technique for the analyses of many natural alpha nuclides and also the beta nuclide 90Sr. The principle is based on a selective extraction of the radionuclide from the water phase by means of a complexing or ion pair reagent. The extractant contains also a cocktail suitable for scintillation counting. Therefore, the extract can be analyzed directly after the extraction step. After removing quenchers, such as oxygen, and the proper setting of a pulse shape discriminator, alpha pulses can be counted with a photomultiplier. This paper describes the development of robust analysis schemes for the determination of traces of polonium, thorium, uranium and other actinides in water samples (groundwater, rain water, river water, drinking water, mineral water, sea water). For radon and radium, the enrichment in the extract is poor. Therefore, PERALS methods are not suitable for trace analyses of these analytes. In addition, the extraction of the beta-emitter 90Sr with a PERALS cocktail is discussed, even though its beta spectrum is not analyzed with a PERALS counter. Results from the survey of drinking water and mineral water in Switzerland are presented for every radio element.

Applications of PERALS-Alpha Spectrometry for the Investigation of Radionuclides in Water Samples

In this paper, experiences of the last 20 years with the PERALS-technique are described. PERALS stands for Photo Electron Rejecting Alpha Liquid Scintillation. This LSC-technique was developed by Jack McDowell in the 70ies and is a powerful technique for the analyses of many natural alpha nuclides. The principle is based on a selective extraction of the radionuclide from the water phase by means of a complexing or ion pair reagent. The extractant contains also a suitable cocktail for the scintillation counting. Therefore, the extract can be analysed directly after the extraction step. After removing quenchers, such as oxygen, and the proper setting of a pulse shape discriminator, alpha pulses can be counted with a photomultiplier. The paper describes the development of robust analysis schemes for the determination of traces of polonium, radon, radium, strontium, thorium, uranium and other actinides in water samples (groundwater, rain water, river water, drinking water, mineral water, sea water).

Powierzchniowy a katastralny system opodatkowania nieruchomości w Polsce

The article explores the economic aspects of area and value-based taxation of Polish real estate. It begins with the presentation of information on the conditions of property taxation in Poland. Next, a review of the research on the cadastral tax is provided, as well as the assumptions of the econometric model used for mass valuation of the analysed real estate. The main part of the article contains the results of the simulation of replacing the property tax with an ad valorem one. Particular attention is given to the impact of the cadastral tax rate on the revenues of municipalities and changes in the tax burden on individual properties. The main aim of this study was to show the importance of the proper setting of the ad valorem tax rate in the process of reforming the property taxation system.

Dependences between the footwear fitting and feet structure in girls of third-grade primary school

Background: The study aimed to study dependences between the footwear fitting and feet structure in girls of third grade of primary school. Material and methods: The research tool was the podoscope CQ-ST. The footwear fitting to the feet of the examined children was tested using the Clevermess device. Results: Statistically significant relationships between the frequency of varus deformity of the Vth toe of the right foot and the width fitting of the outdoor footwear were found (p=0.027). Statistically significant relationships between the length of the indoor footwear and the heel angle (γ) of the left foot have also been noted (p = 0.038). Conclusions: Conclusions: Children with a wider forefoot wear longer shoes, which is why there is a need to take into account differences in the foot width in the production of footwear used indoors. The use of too short footwear, without free space that is necessary for the proper setting of the foot during movement or load, is a factor leading to excessive lateral arching. Wearing too wide shoes affects the formation of varus deformity of the Vth toe of the right foot.

Sensory active substances causing off-odour in liquid whey during storage

Liquid whey is a nutritious product with high water activity and neutral pH. Therefore, it is very susceptible to microbiological spoilage that results in undesirable off-odors. Additionally, minimally processed foods are the recent trend so setting an appropriate shelf life is essential. The commonly used microbiological methods are lengthy and time-demanding, so a quick and early identification of microbial degradation would be a significant benefit. Here we tested a solid-phase microextraction, gas chromatography with mass spectrometry coupled with olfactometry analysis (SPME-GC-MS/O) on samples of sweet unpasteurized liquid whey stored at 6 °C, 12 °C and 25 °C for a week. We compared the common methods – plate methods, measurement of pH, and dry matter determination with our proposed SPME-GC-MS/O. We have identified seven sensory active compounds while octanoic acid and a compound not reliably identified by the MS detector (with main m/z observed 133 (100), 151 (65), and 135 (26)) being the most prominent. Microbiological methods proved irreplaceable for proper setting of storage conditions (with the growth of coliforms being significant (p <0.001) at 25 °C). However, SPME-GC-MS/O was able to identify volatile substances responsible for off-odors and can be used as a powerful tool to detect the cause of undesirable chemical and microbial changes in whey beverages.

Determination of SCS-CN initial abstraction ratio in a catchment prone to flash floods

The soil conservation service - curve number method is a globally used approach to simulations of surface runoff for its simplicity and applicability. Nevertheless, relevant simulations require proper setting of the model's components. This work focuses on optimization of initial abstraction ratio λ in the Husí potok sub-catchments in Czech Republic. Due to favorable morphology, the watershed is prone to flash floods and accurate modeling of surface runoff is of high interest. The analysis was conducted using pairs of discharge and rainfall measurements. The results outline that the traditional value λ= 0.2 is too high in this watershed and should be reduced.

Physical Rigging Procedures Based on Character Type and Design in 3D Animation

In 3D (Three-Dimensional) animation pipeline, the term rigging is commonly used to describe the process of creating skeleton system inside the finishing 3D character geometry and assigning animation controllers for animators to animate the 3D character. In order to establish a fluidity movement on the character, the proper setting must be planned and the flexibility of the controller must be setup properly. However, the discussion and understanding about rigging setup for specific 3D characters from previous researcher is very limited. Hence, the process of rigging will take longer time to complete especially for novice rigger artist. This study will explore the fundamental process of character rigging system and identify the method and techniques for rigging characters based on their anatomy designs. The analysis of this study will look on the conceptual design of the character; how to implement proper techniques and workflow based on the primary and secondary data provided in this study. The findings in this paper indicate common techniques for human rig object in 3D characters is using Biped, CAT or auto rigs. From our experiment, we found biped or CAT rigging is the best setting for the animals and realism characters. Custom rig is suitable for cartoony characters. Rigging system for anthropomorphism character is a bit different from the human rigs system. Anthropomorphism character requires several modifications on their physical appearances in which adding or subtracting the limb that physically taken from specific animal or any objects. For this type of character, we suggested to use biped rigging system. However, for complex anthropomorphism characters with many form structures, the study of movement, exaggeration and bone limitation must be analyzed before deciding the proper technique for rigging. Hence, the chain rig system is the best option for this type of anthropomorphism character. Therefore, rigging in animation is the process assembling the skeleton system into the character. The use of proper rigging style will determine how the character move and pose in final animation.

Influence of Distance from the Host on Parasitisation by Psyttalia concolor (Szépligeti) (Hymenoptera: Braconidae)

The olive fruit fly, Bactrocera oleae, is considered the main olive pest worldwide, and has been the target of biological control programmes through the release of the braconid parasitoid Psyttalia concolor. Laboratory tests were performed to evaluate the influence of distance from the host on parasitisation, placing larvae of the substitute host Ceratitis capitata at seven distances (0, 0.5, 1, 1.5, 2, 2.5, 3 mm) and four different time periods (7, 15, 30, 60 min). Moreover, field collected olives of Ogliarola Barese cultivar infested by B. oleae were exposed to P. concolor females to confirm its ability to parasitise B. oleae in small olives. Psyttalia concolor oviposition was inhibited at 2.5 and 3 mm due to the ovipositor length of the parasitoid females (2.7 mm). Hosts were easily parasitised at distances between 0 and 1.5 mm. The thin fruit pulp (up to 3.5 mm) of field collected olives allowed the parasitisation to occur also in mature fruits. At the best combination distance/time (0 mm, 30 min), tests performed with different larvae/parasitoid female ratio showed an increasing emergence of P. concolor (from 20% to 57%) with larvae/parasitoid ratio increasing from 0.11 to 0.74. The results of the present study might optimise the mass rearing of P. concolor, through a proper setting of its parameters, such as the host/parasitoid ratio, exposure distances, and interaction time.

DeRPN: Taking a Further Step toward More General Object Detection

Most current detection methods have adopted anchor boxes as regression references. However, the detection performance is sensitive to the setting of the anchor boxes. A proper setting of anchor boxes may vary significantly across different datasets, which severely limits the universality of the detectors. To improve the adaptivity of the detectors, in this paper, we present a novel dimension-decomposition region proposal network (DeRPN) that can perfectly displace the traditional Region Proposal Network (RPN). DeRPN utilizes an anchor string mechanism to independently match object widths and heights, which is conducive to treating variant object shapes. In addition, a novel scale-sensitive loss is designed to address the imbalanced loss computations of different scaled objects, which can avoid the small objects being overwhelmed by larger ones. Comprehensive experiments conducted on both general object detection datasets (Pascal VOC 2007, 2012 and MS COCO) and scene text detection datasets (ICDAR 2013 and COCO-Text) all prove that our DeRPN can significantly outperform RPN. It is worth mentioning that the proposed DeRPN can be employed directly on different models, tasks, and datasets without any modifications of hyperparameters or specialized optimization, which further demonstrates its adaptivity. The code has been released at https://github.com/HCIILAB/DeRPN.