scholarly journals Growth and Maintenance of Wolbachia in Insect Cell Lines

Insects ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 706
Author(s):  
Ann M. Fallon
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Cultured Cells ◽  
Biological Control Agent ◽  
Wolbachia Infection ◽  
Control Agent ◽  
Host Cells ◽  
Gene Drive ◽  
Insect Cell Lines

The obligate intracellular microbe, Wolbachia pipientis (Rickettsiales; Anaplasmataceae), is a Gram-negative member of the alpha proteobacteria that infects arthropods and filarial worms. Although closely related to the genera Anaplasma and Ehrlichia, which include pathogens of humans, Wolbachia is uniquely associated with invertebrate hosts in the clade Ecdysozoa. Originally described in Culex pipiens mosquitoes, Wolbachia is currently represented by 17 supergroups and is believed to occur in half of all insect species. In mosquitoes, Wolbachia acts as a gene drive agent, with the potential to modify vector populations; in filarial worms, Wolbachia functions as a symbiont, and is a target for drug therapy. A small number of Wolbachia strains from supergroups A, B, and F have been maintained in insect cell lines, which are thought to provide a more permissive environment than the natural host. When transferred back to an insect host, Wolbachia produced in cultured cells are infectious and retain reproductive phenotypes. Here, I review applications of insect cell lines in Wolbachia research and describe conditions that facilitate Wolbachia infection and replication in naive host cells. Progress in manipulation of Wolbachia in vitro will enable genetic and biochemical advances that will facilitate eventual genetic engineering of this important biological control agent.

scholarly journals In vitro infection of Wolbachia in insect cell lines

2008 ◽  
Vol 43 (4) ◽  
pp. 519-525 ◽  
Author(s):  
Seiichi Furukawa ◽  
Kohjiro Tanaka ◽  
Takema Fukatsu ◽  
Tetsuhiko Sasaki
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Insect Cell Lines

scholarly journals In vitro phagocytosis of occlusion bodies of nucleopolyhedroviruses by insect cell lines.

2001 ◽  
Vol 36 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Hsi-Nan Yang ◽  
Chu-Fang Lo ◽  
Ching-Yen Lin ◽  
Pi-Fen Tsae ◽  
Chung-Hsiung Wang
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Occlusion Bodies ◽  
Insect Cell Lines

scholarly journals Aphid-Symbiotic Bacteria Cultured in Insect Cell Lines

2005 ◽  
Vol 71 (8) ◽  
pp. 4833-4839 ◽  
Author(s):  
A. C. Darby ◽  
S. M. Chandler ◽  
S. C. Welburn ◽  
A. E. Douglas
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Cell Types ◽  
Symbiotic Bacteria ◽  
Rrna Gene ◽  
Animal Cells ◽  
Genomic Studies ◽  
Insect Cell Lines ◽  
Cultivation In Vitro

ABSTRACT The cells and tissues of many aphids contain bacteria known as “secondary symbionts,” which under specific environmental circumstances may be beneficial to the host insect. Such symbiotic bacteria are traditionally described as intractable to cultivation in vitro. Here we show that two types of aphid secondary symbionts, known informally as T type and U type, can be cultured and maintained in three insect cell lines. The identities of the cultured bacteria were confirmed by PCR with sequencing of 16S rRNA gene fragments and fluorescence in situ hybridization. In cell lines infected with bacteria derived from aphids harboring both T type and U type, the U type persisted, while the T type was lost. We suggest that the two bacteria persist in aphids because competition between them is limited by differences in tropism for insect tissues or cell types. The culture of these bacteria in insect cell lines provides a new and unique research opportunity, offering a source of unibacterial material for genomic studies and a model system to investigate the interactions between animal cells and bacteria. We propose the provisional taxon names “Candidatus Consessoris aphidicola” for T type and “Candidatus Adiaceo aphidicola” for U type.

In vitro host range of the Hz-1 nonoccluded virus in insect cell lines

2007 ◽  
Vol 43 (5-6) ◽  
pp. 196-201 ◽  
Author(s):  
Arthur H. McIntosh ◽  
James J. Grasela ◽  
Carlo M. Ignoffo
Keyword(s):  
Host Range ◽  
Cell Lines ◽  
Insect Cell ◽  
Insect Cell Lines

The effect of the hyperparasite (Gliocladium virens) on Rhizoctonia solani and on Rhizoctonia root rot of white beans

1981 ◽  
Vol 59 (1) ◽  
pp. 22-27 ◽  
Author(s):  
J. C. Tu ◽  
O. Vaartaja
Keyword(s):  
Rhizoctonia Solani ◽  
Root Rot ◽  
Biological Control Agent ◽  
Light And Electron Microscopy ◽  
Control Agent ◽  
Host Cells ◽  
Sequence Of Events ◽  
Rhizoctonia Root Rot ◽  
Gliocladium Virens

Using light and electron microscopy, Gliocladium virens is proved to be a hyperparasite of Rhizoctonia solani. When hyphae of G. virens come in contact with those of R. solani, the following sequence of events are observed (i) contact of G. virens with host cells, (ii) formation of appresoria, (iii) penetration of host cells, (iv) formation of intracellular hyphae, and (v) collapse and death of host cells. In vitro, G. virens effectively inhibited sclerotial formation of R. solani.Greenhouse tests showed that the presence of G. virens in soil artificially infested with R. solani reduced at planting the severity of Rhizoctonia root rot in white beans. Root rot severity decreased with increasing concentrations of G. virens. A similar result was obtained in soil treated with the two fungi 2 months prior to planting. This study suggests that G. virens may be a promising biological control agent for Rhizoctonia root rot of white beans.

Toxicity of Pyrrolizidine Alkaloids to Spodoptera exigua Using Insect Cell Lines and Injection Bioassays

2014 ◽  
Vol 40 (6) ◽  
pp. 609-616 ◽  
Author(s):  
Tri R. Nuringtyas ◽  
Robert Verpoorte ◽  
Peter G. L. Klinkhamer ◽  
Monique M. van Oers ◽  
Kirsten A. Leiss
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Pyrrolizidine Alkaloids ◽  
Spodoptera Exigua ◽  
Insect Cell Lines
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