scholarly journals Aphid-Symbiotic Bacteria Cultured in Insect Cell Lines

2005 ◽  
Vol 71 (8) ◽  
pp. 4833-4839 ◽  
Author(s):  
A. C. Darby ◽  
S. M. Chandler ◽  
S. C. Welburn ◽  
A. E. Douglas
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Cell Types ◽  
Symbiotic Bacteria ◽  
Rrna Gene ◽  
Animal Cells ◽  
Genomic Studies ◽  
Insect Cell Lines ◽  
Cultivation In Vitro

ABSTRACT The cells and tissues of many aphids contain bacteria known as “secondary symbionts,” which under specific environmental circumstances may be beneficial to the host insect. Such symbiotic bacteria are traditionally described as intractable to cultivation in vitro. Here we show that two types of aphid secondary symbionts, known informally as T type and U type, can be cultured and maintained in three insect cell lines. The identities of the cultured bacteria were confirmed by PCR with sequencing of 16S rRNA gene fragments and fluorescence in situ hybridization. In cell lines infected with bacteria derived from aphids harboring both T type and U type, the U type persisted, while the T type was lost. We suggest that the two bacteria persist in aphids because competition between them is limited by differences in tropism for insect tissues or cell types. The culture of these bacteria in insect cell lines provides a new and unique research opportunity, offering a source of unibacterial material for genomic studies and a model system to investigate the interactions between animal cells and bacteria. We propose the provisional taxon names “Candidatus Consessoris aphidicola” for T type and “Candidatus Adiaceo aphidicola” for U type.

scholarly journals In vitro infection of Wolbachia in insect cell lines

2008 ◽  
Vol 43 (4) ◽  
pp. 519-525 ◽  
Author(s):  
Seiichi Furukawa ◽  
Kohjiro Tanaka ◽  
Takema Fukatsu ◽  
Tetsuhiko Sasaki
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Insect Cell Lines

Infection, growth and maintenance of Wolbachia pipientis in clonal and non-clonal Aedes albopictus cell cultures

2012 ◽  
Vol 103 (3) ◽  
pp. 251-260 ◽  
Author(s):  
C.C.H. Khoo ◽  
C.M.P. Venard ◽  
Y. Fu ◽  
D.R. Mercer ◽  
S.L. Dobson
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Aedes Albopictus ◽  
Cell Types ◽  
Infection Level ◽  
Wolbachia Pipientis ◽  
Albopictus Cell ◽  
Host Cell Interactions ◽  
Insect Cell Lines

AbstractInsect cell lines provide useful in vitro models for studying biological systems, including interactions between mosquitoes and obligate intracellular endosymbionts such as Wolbachia pipientis. The Aedes albopictus Aa23 cell line was the first cell line developed to allow examination of Wolbachia infections. However, Wolbachia studies using Aa23 can be complicated by the presence of different cell types in the cell line and the substantial temporal variation in infection level. Two approaches were examined to ameliorate infection variability. In the first approach, multiple Aa23 passaging regimes were tested for an effect on infection variability. Fluorescence in situ hybridization (FISH) staining was used to characterize Wolbachia infection level over time. The results demonstrate an impact of passaging method on Wolbachia infection level, with some methods resulting in loss of infection. None of the passaging methods succeeded in effectively mitigating infection level variation. In a second approach, the clonal C7-10 A. albopictus cell line was infected with Wolbachia from Aa23 cells and Drosophila simulans (Riverside), resulting in cell lines designated C7-10B and C7-10R, respectively. Characterization via FISH staining showed greater stability and uniformity of Wolbachia infection in C7-10R relative to the infection in C7-10B. Characterization of the Aa23, C7-10B and C7-10R lines is discussed as a tool for the study of Wolbachia-host cell interactions.

scholarly journals In vitro phagocytosis of occlusion bodies of nucleopolyhedroviruses by insect cell lines.

2001 ◽  
Vol 36 (1) ◽  
pp. 59-69 ◽  
Author(s):  
Hsi-Nan Yang ◽  
Chu-Fang Lo ◽  
Ching-Yen Lin ◽  
Pi-Fen Tsae ◽  
Chung-Hsiung Wang
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Occlusion Bodies ◽  
Insect Cell Lines

Recovery of infective juveniles of the entomopathogenic nematode Steinernema carpocapsae via factors produced by insect cells and symbiotic bacteria

Nematology ◽  
2009 ◽  
Vol 11 (4) ◽  
pp. 611-618 ◽  
Author(s):  
Shingo Kikuta ◽  
Takashi Kiuchi ◽  
Fugaku Aoki ◽  
Masao Nagata
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Mammalian Cells ◽  
Insect Cells ◽  
Entomopathogenic Nematode ◽  
Insect Cell Line ◽  
Fruit Fly ◽  
Steinernema Carpocapsae ◽  
Symbiotic Bacteria ◽  
Insect Cell Lines

Abstract Entomopathogenic nematodes, Steinernema carpocapsae, show 'recovery' from the dauer form as infective juveniles (IJ) up to fourth-stage juveniles when host invasion occurs. This recovery also occurs within an insect cell line culturing system. Here we addressed the factor(s) that induce recovery. When IJ were exposed to cell medium obtained from the cultivation of Sf9 cell lines derived from armyworms (Spodoptera frugiperda), approximately 50% of IJ recovered after 4 h. By 16 h, 90% of the IJ had undergone recovery. Other insect cell lines such as silkworm (Bombyx mori)-derived BmN cells and fruit fly (Drosophila melanogaster)-derived S2 cells also secreted the recovery inducing factor(s). By contrast, mammalian cells (NIH/3T3 and HeLa) had no effect on nematode recovery. Our data also suggest that symbiotic bacteria are involved in IJ recovery; axenic IJ did not recover in the cell-cultured medium. When symbiotic bacteria isolated from IJ were propagated within the cell-cultured medium, the supernatant gained recovery-inducing activity against axenic IJ. From these results, we conclude that IJ recovery in S. carpocapsae is induced by multiple factor(s) secreted from insect cells and symbiont bacteria.

In vitro host range of the Hz-1 nonoccluded virus in insect cell lines

2007 ◽  
Vol 43 (5-6) ◽  
pp. 196-201 ◽  
Author(s):  
Arthur H. McIntosh ◽  
James J. Grasela ◽  
Carlo M. Ignoffo
Keyword(s):  
Host Range ◽  
Cell Lines ◽  
Insect Cell ◽  
Insect Cell Lines

scholarly journals Growth and Maintenance of Wolbachia in Insect Cell Lines

Insects ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 706
Author(s):  
Ann M. Fallon
Keyword(s):  
Cell Lines ◽  
Insect Cell ◽  
Cultured Cells ◽  
Biological Control Agent ◽  
Wolbachia Infection ◽  
Control Agent ◽  
Host Cells ◽  
Gene Drive ◽  
Insect Cell Lines

The obligate intracellular microbe, Wolbachia pipientis (Rickettsiales; Anaplasmataceae), is a Gram-negative member of the alpha proteobacteria that infects arthropods and filarial worms. Although closely related to the genera Anaplasma and Ehrlichia, which include pathogens of humans, Wolbachia is uniquely associated with invertebrate hosts in the clade Ecdysozoa. Originally described in Culex pipiens mosquitoes, Wolbachia is currently represented by 17 supergroups and is believed to occur in half of all insect species. In mosquitoes, Wolbachia acts as a gene drive agent, with the potential to modify vector populations; in filarial worms, Wolbachia functions as a symbiont, and is a target for drug therapy. A small number of Wolbachia strains from supergroups A, B, and F have been maintained in insect cell lines, which are thought to provide a more permissive environment than the natural host. When transferred back to an insect host, Wolbachia produced in cultured cells are infectious and retain reproductive phenotypes. Here, I review applications of insect cell lines in Wolbachia research and describe conditions that facilitate Wolbachia infection and replication in naive host cells. Progress in manipulation of Wolbachia in vitro will enable genetic and biochemical advances that will facilitate eventual genetic engineering of this important biological control agent.

scholarly journals A study of communication specificity between cells in culture.

1977 ◽  
Vol 75 (3) ◽  
pp. 769-787 ◽  
Author(s):  
M L Epstein ◽  
N B Gilula
Keyword(s):  
Gap Junctions ◽  
Cell Lines ◽  
Insect Cell ◽  
Cell Types ◽  
Cells In Culture ◽  
Culture Cells ◽  
Low Incidence ◽  
Insect Cell Lines ◽  
Gap Junctional ◽  
Ionic Coupling

We have examined the specificity of communication between cells in culture by co-culturing cells derived from mammalian, avian, and arthropod organisms. Both mammalian and avian culture cells have similar gap junctional phenotypes, while the insect (arthropod) cell lines have a significantly different gap junctional structure. Electrophysiological and ultrastructural methods were used to examine ionic coupling and junctional interactions between homologous and heterologous cell types. In homologous cell systems, gap junctions and ionic coupling are present at a high incidence. Also, heterologous vertebrate cells in co-culture can communicate readily. By contrast, practically no coupling (0-8%) is detectable between heterologous insect cell lines (Homopteran or Lepidopteran) and vertebrate cells (mammalian myocardial or 3T3 cells). No gap junctions have been observed between arthropod and vertebrate cell types, even though the heterologous cells may be separated by less than 10 nm. In additional studies, a low incidence of coupling was found between heterologous insect cell lines derived from different arthropod orders. However, extensive coupling was detected between insect cell lines that are derived from the same order (Homoptera). These observations suggest that there is little or no apparent specificity for communication between vertebrate cells in culture that express the same gap junctional phenotype, while there is a definite communication specificity that exists between arthropod cells in culture.

Sign in / Sign up

Export Citation Format

Share Document