Feasibility Study on the Use of Fly Maggots (Musca domestica) as Carriers to Inhibit Shrimp White Spot Syndrome

The shrimp aquaculture industry has encountered many diseases that have caused significant losses, with the most serious being white spot syndrome (WSS). Until now, no cures, vaccines, or drugs have been found to counteract the WSS virus (WSSV). The purpose of this study was to develop an oral delivery system to transport recombinant proteinaceous antigens into shrimp. To evaluate the feasibility of the oral delivery system, we used white shrimp as the test species and maggots as protein carriers. The results indicated that the target protein was successfully preserved in the maggot, and the protein was detected in the gastrointestinal tract of the shrimp, showing that this oral delivery system could deliver the target protein to the shrimp intestine, where it was absorbed. In addition, the maggots were found to increase the total haemocyte count and phenoloxidase activity of the shrimp, and feeding shrimp rVP24-fed maggots significantly induced the expression of penaeidins 2. In the WSSV challenge, the survival rate of rVP24-fed maggots was approximately 43%. This study showed that maggots can be used as effective oral delivery systems for aquatic products and may provide a new method for aquatic vaccine delivery systems.

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An oral delivery system for controlling white spot syndrome virus infection in shrimp using transgenic microalgae

Aquaculture ◽

10.1016/j.aquaculture.2020.735022 ◽

2020 ◽

Vol 521 ◽

pp. 735022 ◽

Cited By ~ 3

Author(s):

Asama Kiataramgul ◽

Sugunya Maneenin ◽

Saul Purton ◽

Nontawith Areechon ◽

Ikuo Hirono ◽

...

Keyword(s):

Virus Infection ◽

Delivery System ◽

Oral Delivery ◽

White Spot Syndrome Virus ◽

White Spot ◽

Oral Delivery System ◽

White Spot Syndrome

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In vitro Evaluation of Arabinoxylan Gels as an Oral Delivery System for Insulin

MRS Proceedings ◽

10.1557/opl.2012.1529 ◽

2012 ◽

Vol 1487 ◽

Cited By ~ 3

Author(s):

E. Carvajal-Millan ◽

C. Berlanga-Reyes ◽

A. Rascón-Chu ◽

A. L. Martínez-López ◽

J. A. Márquez-Escalante ◽

...

Keyword(s):

Diffusion Coefficient ◽

Apparent Diffusion Coefficient ◽

Delivery System ◽

Oral Delivery ◽

Delivery Systems ◽

Oral Delivery System ◽

Apparent Diffusion ◽

Oral Delivery Systems ◽

Hydrolysis Of

ABSTRACTArabinoxylan gels are receiving increasing attention as oral delivery systems of biomolecules for therapeutic purposes. The aim of this research was to evaluate arabinoxylan gels as an oral delivery system for insulin, representing a painless therapy for diabetics. Gels at two concentrations of arabinoxylan were prepared (2.5 and 3.5 % w/v). One concentration of insulin (0.05 % w/v) entrapped in the arabinoxylan gels was investigated. At the end of gelation elasticity (G’) values were 11 and 20 for gels at 2.5 and 3.5% (w/v) in arabinoxylan, respectively. The presence of insulin in the gels did not affect the values of G’. The apparent diffusion coefficient for insulin decreased from 1.30 x 10-7 to 1.09 x 10-7 cm2/s when the concentration of arabinoxylan in the gel increased from 2.5 to 3.5% (w/v). The percentage of proteolysis for insulin entrapped in the gels at 2.5 and 3.5% in arabinoxylan (w/v) were 35 and 17%, respectively, in relation to 100% hydrolysis of insulin in solution. Results indicate that arabinoxylan gels could be potential candidates as oral delivery systems for insulin.

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Chitosan and Quercetin: Potential Hand in Hand Encountering Tumors in Oral Delivery System

Current Pharmaceutical Design ◽

10.2174/1381612825666190829144508 ◽

2019 ◽

Vol 25 (28) ◽

pp. 3074-3086

Author(s):

Jalil Rashedi ◽

Amir Ghorbanihaghjo ◽

Mohammad Asgharzadeh ◽

Behzad Baradaran

Keyword(s):

Physicochemical Properties ◽

Delivery System ◽

Oral Delivery ◽

Delivery Systems ◽

Target Site ◽

Cross Linking ◽

Oral Delivery System ◽

Polymeric Compounds ◽

Oral Delivery Systems ◽

Natural And Synthetic

: Chitosan is a cationic polysaccharide and multi-potential polymer with the ability to interact with other natural and synthetic polyanionic/polymeric compounds, with or without a cross-linking agent. It has been able to composite nano-microparticles with a variety of features. This compound has the ability to carry quercetin, as an anti-tumor subject, through the various epithelial systems and release in the sustained and controlled state to the target site. : This paper reviews published studies on detailed physicochemical properties of chitosan and quercetin in the fight against the tumor. This also focused on how the chitosan polymer interacts with other polymeric and polyanion species in order to improve its efficiency to make a more suitable capsule or matrix for a variety of drugs, especially quercetin, in oral delivery systems.

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Oral cyclosporine A - the current picture of its liposomal and other delivery systems

Cellular & Molecular Biology Letters ◽

10.2478/s11658-008-0041-6 ◽

2009 ◽

Vol 14 (1) ◽

Cited By ~ 35

Author(s):

Aleksander Czogalla

Keyword(s):

Autoimmune Diseases ◽

Physicochemical Properties ◽

Organ Transplantation ◽

Delivery System ◽

Cyclosporine A ◽

Lipid Membranes ◽

Oral Delivery ◽

Delivery Systems ◽

Oral Delivery System ◽

Low Solubility

AbstractThe discovery of cyclosporine A was a milestone in organ transplantation and the treatment of autoimmune diseases. However, developing an efficient oral delivery system for this drug is complicated by its poor biopharmaceutical characteristics (low solubility and permeability) and the need to carefully monitor its levels in the blood. Current research is exploring various approaches, including those based on emulsions, microspheres, nanoparticles, and liposomes. Although progress has been made, none of the formulations is flawless. This review is a brief description of the main pharmaceutical systems and devices that have been described for the oral delivery of cyclosporine A in the context of the physicochemical properties of the drug and the character of its interactions with lipid membranes.

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