scholarly journals Gas Chromatography–Mass Spectrometry-Based Metabolomic Analysis of Wagyu and Holstein Beef

Metabolites ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 95
Author(s):  
Tomoya Yamada ◽  
Mituru Kamiya ◽  
Mikito Higuchi
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Intramuscular Fat ◽  
Sweet Taste ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Metabolomic Analysis ◽  
Japanese Black Cattle ◽  
Chromatography Mass Spectrometry ◽  
Intramuscular Fat Content

Japanese Black cattle (Wagyu) beef is characterized by high intramuscular fat content and has a characteristic sweet taste. However, the chemical components for characterizing the sweet taste of Wagyu beef have been unclear. In this experiment, we conducted a metabolomic analysis of the longissimus muscle (sirloin) in Wagyu and Holstein cattle to determine the key components associated with beef taste using gas chromatography–mass spectrometry (GC-MS). Holstein sirloin beef was characterized by the abundance of components such as glutamine, ribose-5-phosphate, uric acid, inosine monophosphate, 5-oxoproline, and glycine. In contrast, Wagyu sirloin beef was characterized by the abundance of sugar components (maltose and xylitol). Dietary fat is known to increase the intensity of sweet taste. These results suggest that the sweet taste of Wagyu beef is due to the synergetic effects of higher sugar components and intramuscular fat.

scholarly journals Metabolomic Analysis of Gingival Crevicular Fluid Using Gas Chromatography/Mass Spectrometry

2016 ◽  
Vol 5 (1) ◽  
pp. A0047-A0047 ◽  
Author(s):  
Miho Ozeki ◽  
Takenori Nozaki ◽  
Jun Aoki ◽  
Takeshi Bamba ◽  
Kirk R. Jensen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gingival Crevicular Fluid ◽  
Gas Chromatography Mass Spectrometry ◽  
Metabolomic Analysis ◽  
Chromatography Mass Spectrometry ◽  
Crevicular Fluid

scholarly journals ANALYSIS OF CHEMICAL COMPOUNDS OF AGARWOOD OIL FROM DIFFERENT SPECIES BY GAS CHROMATOGRAPHY MASS SPECTROMETRY (GCMS)

2014 ◽  
Vol 15 (1) ◽  
Author(s):  
Yumi Zuhanis Has-Yun Hashim ◽  
Nur Izzah Ismail ◽  
Phirdaous Abbas
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Chromatography Mass Spectrometry ◽  
Three Samples ◽  
Pleasant Odour ◽  
Agarwood Resin ◽  
Agarwood Oil

ABSTRACT: Agarwood oil is a highly prized type of oil due to its unique aroma. The oil is extracted from the fragrant resin found in the agarwood tree (trunk).  The unique aroma and quality of agarwood resin and oil are contributed by the presence of certain chemical compounds. In this work, analysis and comparison of the chemical compounds of agarwood oil from A. malaccensis, A. sub-integra and a mixture of both were conducted.  The essential oils were diluted in hexane (5%) prior to gas chromatography mass spectrometry (GCMS) analysis performed using Agilent GCMS 7890A coupled with MSD quadrupole detector 5975 C.  Separation of analytes by gas chromatography was carried out using a Hewlett Packard HP-5MS silica capillary column (30 m X 0.25 mm X 0.25 mm). A total of 107 compounds were identified from the three samples of agarwood oils. Fifty-five (55) components were identified in A. malaccensis sample which contributes to the largest portion of the total compounds. About 20% of the compounds identified were aromatic and sesquiterpenes which have been revealed to be the main active compounds of agarwood oils which also give the aroma and pleasant odour of agarwood. Different compositions or profile of chemical components were found in agarwood oils from the two different species. Two compounds were commonly identified in all three samples namely 3-phenyl-2-butanone and alpha-cubebene.  Further studies are needed to refine the results which later can be used to assist detection and authentication of agarwood as well as its scientific-based grading. ABSTRAK: Minyak gaharu merupakan sejenis minyak beraroma unik yang mendapat permintaan tinggi dan mahal. Minyak ini diekstrak daripada resin beraroma yang terbentuk di dalam batang pokok gaharu. Keunikan aroma dan kualiti resin dan minyak gaharu ini bergantung kepada kehadiran bahan kimia tertentu. Penyelidikan ini menjurus kepada analisis dan perbandingan bahan-bahan kimia yang terdapat dalam minyak gaharu daripada spesis A. malaccensis, A. sub-integra dan campuran kedua-duanya. Minyak gaharu dilarutkan di dalam 5% heksana sebelum dianalisis menggunakan kromatografi gas Agilent GCMS 7890A berserta spektormetri jisim (MSD quadrupole detector 5975 C). Kolum kapilari silika Hewlet Packard HP-5MS (30 m X 0.25 mm X 0.25 mm) digunakan untuk pemisahan bahan-bahan kimia tersebut. Sejumlah 107 kompaun dikenalpasti dalam ketiga-tiga sampel minyak gaharu tersebut dengan 55 kompaun didapati di dalam sample A. malaccensis. Ini merupakan bahagian terbesar daripada keseluruhan kompaun. Sebanyak 20 % daripada keseluruhan kompaun yang dikenalpasti adalah daripada kumpulan seskuiterpena dan bersifat aromatik, juga merupakan kumpulan yang bertanggungjawab memberi aroma yang unik kepada minyak gaharu. Setiap spesis didapati mempunyai komposisi atau profil bahan kimia yang tersendiri. Dua kompaun iaitu 3-phenyl-2-butanone dan alpha-cubebene ditemui dalam ketiga-tiga sample yang dianalisis. Kajian lanjut adalah perlu untuk memurnikan dapatan hasil penyelidikan yang berpotensi bagi membantu pengenalpastian gaharu berserta pembangunan penggredan gaharu yang lebih saintifik.

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