scholarly journals Chlamydia trachomatis Whole-Proteome Microarray Analysis of The Netherlands Chlamydia Cohort Study

2019 ◽  
Vol 7 (12) ◽  
pp. 703 ◽  
Author(s):  
Katrin Hufnagel ◽  
Bernice Hoenderboom ◽  
Christoph Harmel ◽  
Juliane K. Rohland ◽  
Birgit H.B. van Benthem ◽  
...  
Keyword(s):  
Cohort Study ◽  
Chlamydia Trachomatis ◽  
The Netherlands ◽  
Ectopic Pregnancy ◽  
Inflammatory Disease ◽  
Chronic Pelvic Pain ◽  
Serum Samples ◽  
Tubal Factor Infertility ◽  
Tubal Factor ◽  
Infection Markers

Chlamydia trachomatis (Ct) whole-proteome microarrays were utilized to identify antibody patterns associated with infection; pelvic inflammatory disease (PID), tubal factor infertility, chronic pelvic pain (CPP) and ectopic pregnancy in a subsample of the Netherlands Chlamydia cohort study. Serum pools were analyzed on whole-proteome arrays. The 121 most reactive antigens identified during whole-proteome arrays were selected for further analysis with minimized microarrays that allowed for single sera analysis. From the 232 single sera; 145 (62.5%) serum samples were reactive for at least one antigen. To discriminate between positive and negative serum samples; we created a panel of in total 18 antigens which identified 96% of all microarray positive samples. Antigens CT_858; CT_813 and CT_142 were most reactive. Comparison of antibody reactivity’s among women with and without Ct related sequelae revealed that the reactivity of CT_813 and CT_142 was less common among women with PID compared to women without (29.0% versus 58.6%, p = 0.005 and 25.8% versus 50.6%, p = 0.017 respectively). CT_858 was less common among CPP cases compared to controls (33.3% versus 58.6; p = 0.028). Using a whole-proteome array to select antigens for minimized arrays allows for the identification of novel informative antigens as general infection markers or disease associated antigens

scholarly journals Relation between Chlamydia trachomatis infection and pelvic inflammatory disease, ectopic pregnancy and tubal factor infertility in a Dutch cohort of women previously tested for chlamydia in a chlamydia screening trial

2019 ◽  
pp. sextrans-2018-053778 ◽  
Author(s):  
Bernice M Hoenderboom ◽  
Birgit H B van Benthem ◽  
Jan E A M van Bergen ◽  
Nicole H T M Dukers-Muijrers ◽  
Hannelore M Götz ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Ectopic Pregnancy ◽  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Control Strategies ◽  
Incidence Rates ◽  
Chlamydia Screening ◽  
Chlamydia Trachomatis Infection ◽  
Tubal Factor Infertility ◽  
Tubal Factor

ObjectivesA better understanding of Chlamydia trachomatis infection (chlamydia)–related sequelae can provide a framework for effective chlamydia control strategies. The objective of this study was to estimate risks and risk factors of pelvic inflammatory disease (PID), ectopic pregnancy and tubal factor infertility (TFI) with a follow-up time of up until 8 years in women previously tested for chlamydia in the Chlamydia Screening Implementation study (CSI) and participating in the Netherlands Chlamydia Cohort Study (NECCST).MethodsWomen who participated in the CSI 2008–2011 (n=13 498) were invited in 2015–2016 for NECCST. Chlamydia positive was defined as a positive CSI-PCR test, positive chlamydia serology and/or self-reported infection (time dependent). Data on PID, ectopic pregnancy and TFI were collected by self-completed questionnaires. Incidence rates and HRs were compared between chlamydia-positive and chlamydia-negative women corrected for confounders.ResultsOf 5704 women included, 29.5% (95% CI 28.3 to 30.7) were chlamydia positive. The incidence rate of PID was 1.8 per 1000 person-years (py) (1.6 to 2.2) overall, 4.4 per 1000 py (3.3 to 5.7) among chlamydia positives compared with 1.4 per 1000 py (1.1 to 1.7) for chlamydia negatives. For TFI, this was 0.4 per 1000 py (0.3 to 0.5) overall, 1.3 per 1000 py (0.8 to 2.1) and 0.2 per 1000 py (0.1 to 0.4) among chlamydia positives and negatives, respectively. And for ectopic pregnancy, this was 0.6 per 1000 py (0.5 to 0.8) overall, 0.8 per 1000 py (0.4 to 1.5) and 0.6 per 1000 py (0.4 to 0.8) for chlamydia negatives. Among chlamydia-positive women, the strongest risk factor for PID was symptomatic versus asymptomatic infection (adjusted HR 2.88, 1.4 to 4.5) and for TFI age <20 versus >24 years at first infection (HR 4.35, 1.1 to 16.8).ConclusionWe found a considerably higher risk for PID and TFI in chlamydia-positive women, but the incidence for ectopic pregnancy was comparable between chlamydia-positive and chlamydia-negative women. Overall, the incidence rates of sequelae remained low.Trial registrationNTR-5597.

scholarly journals Screening of Chlamydia trachomatis and Waddlia chondrophila Antibodies in Women with Tubal Factor Infertility

2020 ◽  
Vol 8 (6) ◽  
pp. 918 ◽  
Author(s):  
Wies T.M. van Dooremalen ◽  
Stephan P. Verweij ◽  
Janneke E. den Hartog ◽  
Carole Kebbi-Beghdadi ◽  
Sander Ouburg ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Pregnancy Outcomes ◽  
Adverse Pregnancy Outcomes ◽  
Intracellular Pathogen ◽  
Serum Samples ◽  
Tubal Factor Infertility ◽  
Fertility Clinic ◽  
Tubal Factor ◽  
Adverse Pregnancy

Waddlia chondrophila is an emerging intracellular pathogen belonging to the order of Chlamydiales, and was previously associated with adverse pregnancy outcomes, as well as tubal factor infertility (TFI). In this study, we investigate the link between both W. chondrophila and Chlamydia trachomatis IgG seropositivity and TFI. Antibodies against both bacteria were measured in 890 serum samples of women visiting a fertility clinic. After a hysterosalpingography and/or laparoscopy, they were classified as either TFI-negative (TFI−) or TFI-positive (TFI+). The total seroprevalence was 13.4% for C. trachomatis and 38.8% for W. chondrophila. C. trachomatis antibodies were present significantly more often in the TFI+ group than in the TFI− group, while for W. chondrophila no difference could be observed. In conclusion, our study confirms the association between C. trachomatis seropositivity and TFI, but no association was found between W. chondrophila seropositivity and TFI. The high percentage of W. chondrophila seropositivity in all women attending a fertility clinic does, however, demonstrate the need for further research on this Chlamydia-like bacterium and its possible role in infertility.

Detection of Chlamydia trachomatis antibodies by 2 novel tests: rELISA and peptide EIA

1998 ◽  
Vol 9 (10) ◽  
pp. 604-607 ◽  
Author(s):  
◽  
◽  
◽  
◽  
◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Inflammatory Disease ◽  
Chlamydial Infection ◽  
Igg Antibodies ◽  
Specific Antibodies ◽  
Tubal Factor Infertility ◽  
Iga Antibodies ◽  
Tubal Factor ◽  
Species Specific

The performance of 2 newly developed enzyme immunoassays (EIAs) intended for the routine serological diagnosis of chlamydial infections was evaluated. rELISA is based on a recombinant lipopolysaccharide antigen which detects chlamydia genus-specific antibodies, and Chlamydia trachomatis EIA is based on a peptide derived from major outer membrane protein and is therefore speciesspecific. Both tests distinguished patients with tubal factor infertility (TFI) or pelvic inflammatory disease (PID) from the controls. The prevalence of IgA antibodies was higher for the PID patients than for the TFI patients; the finding indicates a more active state of infections for the PID patients. Furthermore, C. trachomatis EIA detected more IgG antibodies in the TFI patients than in patients with non-tubal factor infertility. In conclusion, rELISA detected chlamydial antibodies in general, and C. trachomatis EIA detected species-specific antibodies. These EIA tests may be useful in the serodiagnosis of chlamydial infection.

scholarly journals Tubal factor infertility with prior ectopic pregnancy: a double whammy? A retrospective cohort study of 2,892 women

2020 ◽  
Vol 113 (5) ◽  
pp. 1032-1038 ◽  
Author(s):  
He Cai ◽  
Ben W. Mol ◽  
Ping Li ◽  
Xitong Liu ◽  
Antoine Watrelot ◽  
...  
Keyword(s):  
Cohort Study ◽  
Ectopic Pregnancy ◽  
Retrospective Cohort Study ◽  
Retrospective Cohort ◽  
Tubal Factor Infertility ◽  
Tubal Factor
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