Epstein–Barr Virus Infection of Oral Squamous Cells

The Epstein–Barr virus (EBV) is a human herpesvirus associated with various cancers. The number of reports that describe infection of EBV in oral squamous carcinoma cells is increasing. However, there is no available in vitro model to study the possible role of EBV in the development of oral squamous cell carcinoma. Herein, we report establishment of a latent EBV infection of well-differentiated HSC1 cells and poorly differentiated SCC25 cells. Viral copy numbers per cell in EBV-infected HSC1 and SCC25 cells are 2 and 5, respectively. Although the EBV copy number was small, spontaneous viral replication was observed in EBV-infected HSC1 cells. Contrarily, infectious viral production was not observed in EBV-infected SCC25 cells, despite containing larger number of EBV genomes. Chemical activation of cells induced expression of viral lytic BZLF1 gene in EBV-infected HSC1 cells, but not in EBV-infected SCC25 cells. EBV infection activated proliferation and migration of HSC1 cells. However, EBV-infection activated migration but not proliferation in SCC25 cells. In conclusion, EBV can infect squamous cells and establish latent infection, but promotion of cell proliferation and of lytic EBV replication may vary depending on stages of cell differentiation. Our model can be used to study the role of EBV in the development of EBV-associated oral squamous cell carcinoma.

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A novel comparison of Epstein-Barr virus with broad histological spectrum of oral squamous cell carcinoma

Pakistan Journal of Medical Sciences ◽

10.12669/pjms.35.5.899 ◽

2019 ◽

Vol 35 (5) ◽

Author(s):

Muhammad Wasif Saleem ◽

Faraz Ahmed Baig ◽

Naila Irum Hadi

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Buccal Mucosa ◽

Epstein Barr Virus ◽

Histological Subtype ◽

Barr Virus ◽

Ebv Infection ◽

Epstein Barr

Objectives: To evaluate the immunohistochemical expression of Epstein-Barr virus (EBV) in broad spectrum histological subtypes of oral squamous cell carcinoma (OSCC) and to determine the relationship of EBV with clinicopathological parameters of OSCC. Methods: This cross-sectional study included 150 clinically diagnosed OSCC cases from the outpatient of Ziauddin University Hospital from March, 2017 to October, 2018. These were confirmed on histological examination and categorized into conventional squamous cell carcinoma (SCC) and rare variants. Conventional SCC was subcategorized into keratinizing (KSCC), non-keratinizing (NKSCC), and hybrid SCC (HSCC). EBV status was compared among various histological tumor entities and clinicopathological characteristics of OSCC using immunohistochemistry. Chi-square test was used to determine the association of each histological subtype with EBV status with P-value <0.05 considered as statistically significant. Results: Conventional tumor was the most frequent squamous cell carcinoma (n=126; 84%). A significant statistical link of EBV infection was observed with rare histological tumors exhibiting acantholysis (P=0.01), as well as tumors involving buccal mucosa (P=0.03), and habitual smokers (P=0.001). Conclusions: In this study, acantholytic tumor, a rare histological subtype of OSCC, tended to be EBV related. Moreover, OSCC cases bearing EBV infection were more likely smokers favoring buccal mucosa as primary anatomical site for oral cancer. doi: https://doi.org/10.12669/pjms.35.5.899 How to cite this:Saleem MW, Baig FA, Hadi NI. A novel comparison of Epstein-Barr virus with broad histological spectrum of oral squamous cell carcinoma. Pak J Med Sci. 2019;35(5):---------. doi: https://doi.org/10.12669/pjms.35.5.899 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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DETECTION OF EPSTEIN-BARR VIRUS GENOME IN ORAL CAVITY SQUAMOUS CELL CARCINOMA SAMPLES OF RUSSIAN PATIENTS

Voprosy Virusologii ◽

10.18821/0507-4088-2019-64-3-112-117 ◽

2019 ◽

Vol 64 (3) ◽

pp. 112-117 ◽

Cited By ~ 1

Author(s):

S. A. Kiryanov ◽

T. A. Levina ◽

A. P. Polyakov ◽

I. V. Rebrikova ◽

D. A. Murashko ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Oral Cavity ◽

Cell Carcinoma ◽

Squamous Cell ◽

Epstein Barr Virus ◽

Additional Risk Factor ◽

Barr Virus ◽

Epstein Barr

Introduction. Oral cavity squamous cell carcinoma (OC-SCC) is the most common and aggressive malignancy of the oral cavity. Recent studies have revealed infections with human papilloma virus (HPV) as an additional risk factor for oral squamous cell carcinoma development, while distinguished role of Epstein-Barr virus (EBV) remains still uncertain. However, the evidence for association between virus infection and risk of oral squamous cell carcinoma is controversially and varies significantly by geographic regions and race. Purpose. The aim of the present study was to elucidate the prevalence of HPV and EBV in OC-SCC samples of Russian patients from Moscow region. Material and methods. We investigated fresh-frozen tumor tissue fragments obtained from 11 patients with OC-SCC. DNA was extracted and the viral genome was examined by quantitative PCR assays with high-risk type-specific HPV and EBV specific markers followed by sequencing-based analysis. Results. No HPV infection in analyzed OC-SCC samples was observed, while EBV was identified in 70.0% (7/10) of patients. Further based on Q-PCR amplification of the EBV targets including BamHI-W, EBNA1 and C-terminal fragment of LMP1 gene, EBV infection and measurement of virus load in the tumor samples was assessed. Sequencing LMP1-positive products revealed that the most samples (5/6) contained variants LMP1 with Cao deletion characterized by an increased transforming potential. Conclusion. These data suggest that prevalence of EBV infections is common and may influence cancer development, although detected LMP1 variants of EBV are not necessarily associated with the pathogenesis of OC-SCC. Further studies are necessary to determine the potential role of EBV and its possible importance as an infection factor in OC-SCC.

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Etiologic Association between Epstein–Barr Virus and Oral Squamous Cell Carcinoma: A Brief Evidence-based Discussion

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-2028 ◽

2017 ◽

Vol 18 (4) ◽

pp. 261-264

Author(s):

Shankargouda Patil ◽

Barnali Majumdar

Keyword(s):

Squamous Cell Carcinoma ◽

Oral Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Epstein Barr Virus ◽

Evidence Based ◽

Barr Virus ◽

Epstein Barr

How to cite this article Majumdar B, Sarode SC, Sarode GS, Patil S. Etiologic Association between Epstein–Barr Virus and Oral Squamous Cell Carcinoma: A Brief Evidence-based Discussion. J Contemp Dent Pract 2017;18(4):261-264.

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