scholarly journals Linking Shifts in Bacterial Community Composition and Function with Changes in the Dissolved Organic Matter Pool in Ice-Covered Baiyangdian Lake, Northern China

2020 ◽  
Vol 8 (6) ◽  
pp. 883
Author(s):  
Shilei Zhou ◽  
Yue Sun ◽  
Minghui Yu ◽  
Zhenpeng Shi ◽  
Hang Zhang ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacterial Community ◽  
Dissolved Organic Matter ◽  
Bacterial Community Composition ◽  
Environmental Parameters ◽  
Northern China ◽  
Mantel Test ◽  
Biological Index ◽  
Linear Discriminant ◽  
Baiyangdian Lake

The relationship between CDOM (Chromophoric dissolved organic matter) and the bacterial community was investigated in ice-covered Baiyangdian Lake. The results showed that environmental parameters significantly differed in Baiyangdian Lake, whereas a-diversity was not significantly different. Moreover, the microbial and functional communities exhibited significant differences, and T (Temperature), pH, ORP (Oxidation-reduction potential), DO (Dissolved oxygen), NO3−-N, NH4+-N, and Mn (Manganese) were the main environmental factors of these differences, based on redundancy analysis and the Mantel test. Biomarkers of the microbial and functional communities were investigated through linear discriminant analysis effect size and STAMP analysis. The number of biomarkers in the natural area was highest among the typical zones, and most top functions were related to carbohydrate metabolism. Two protein-like components (C1 and C2) and one humic-like component (C3) were identified by parallel factor analysis, and C1 was positively related to C2 (R = 0.99, p < 0.001), indicating the same sources. Moreover, CDOM significantly differed among the typical zones (p < 0.001). The high biological index, fluorescence index, β:α, and low humification index indicated a strong autochthonous component and aquatic bacterial origin, which was consistent with the results of UV-vis absorption spectroscopy. Network analysis revealed non-random co-occurrence patterns. The bacterial and functional communities interacted closely with CDOM. The dominant genera were CL500-29_marine_group, Flavobacterium, Limnohabitans, and Candidatus_Aquirestis. Random forest analysis showed that C1, C2, and C3 are important predictors of α- and β-diversity in the water bacterial community and its functional composition. This study provides insight into the interaction between bacterial communities and DOM (Dissolved organic matter) in ice-covered Baiyangdian Lake.

scholarly journals Experimental insights into the importance of aquatic bacterial community composition to the degradation of dissolved organic matter

2015 ◽  
Vol 10 (3) ◽  
pp. 533-545 ◽  
Author(s):  
Jürg B Logue ◽  
Colin A Stedmon ◽  
Anne M Kellerman ◽  
Nikoline J Nielsen ◽  
Anders F Andersson ◽  
...  
Keyword(s):  
Organic Matter ◽  
Bacterial Community ◽  
Dissolved Organic Matter ◽  
Community Composition ◽  
Bacterial Community Composition

scholarly journals Bacterial Community Composition and Chromophoric Dissolved Organic Matter Differs with Culture Time of Skeletonema dohrnii

Diversity ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 150
Author(s):  
Yang Liu ◽  
Jinjun Kan ◽  
Jing Yang ◽  
Md Abu Noman ◽  
Jun Sun
Keyword(s):  
Organic Matter ◽  
Bacterial Community ◽  
Dissolved Organic Matter ◽  
Community Composition ◽  
Relative Abundance ◽  
Bacterial Community Composition ◽  
Algal Growth ◽  
Chromophoric Dissolved Organic Matter ◽  
Bacterial Phyla ◽  
Culture Time

Skeletonema dohrnii is a common red tide microalgae occurring in the coastal waters and throughout the world. The associated heterotrophic or autotrophic bacteria play vital roles in regulating algal growth, production, and physiology. In this study, we investigated the detailed bacterial community structure associated with the growth of S. dohrnii’s using high-throughput sequencing-based on 16S rDNA. Our results demonstrated that Bacteroidetes (48.04%) and Proteobacteria (40.66%) in all samples accounted for the majority of bacterial populations. There was a significant linear regression relationship between the abundance of bacterial phyla and culture time. Notable shifts in bacterial community composition were observed during algal growth: Flavobacteriales accounted for the vast majority of sequences at the order level. Furthermore, the relative abundance of Rhodobacterales was gradually reduced during the whole growth process of S. dohrnii (0–12 days). However, beyond that, the relative abundance of Marinobacter was slowly increasing. It is noteworthy that five fluorophores (Peaks T1, T2, I, M, and A) were detected during the growth stage of S. dohrnii. The characteristic indexes (fluorescence index, humification index, and biological index) of chromophoric dissolved organic matter (CDOM) also varied with the culture time. In addition, the taxa of bacteria had certain effects on CDOM and they were inextricably linked to each other.

scholarly journals Filtering artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays

2018 ◽  
Author(s):  
Joshua F. Dean ◽  
Jurgen R. van Hal ◽  
Han Dolman ◽  
Rien Aerts ◽  
James T. Weedon
Keyword(s):  
Organic Matter ◽  
Bacterial Community ◽  
Dissolved Organic Matter ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
Amplicon Sequencing ◽  
Microbial Decomposition ◽  
16S Rrna Amplicon Sequencing ◽  
Filter Size ◽  
Carbon Dioxide Co2

Abstract. Inland waters are large contributors to global carbon dioxide (CO2) emissions, in part due to the vulnerability of dissolved organic matter (DOM) to microbial decomposition and respiration to CO2 during transport through aquatic systems. To assess the degree of this vulnerability, aquatic DOM is often incubated in standardized "biolability" assays. These assays isolate the dissolved fraction of aquatic OM by size filtration prior to incubation. We test whether this size selection has an impact on the bacterial community composition and the consequent dynamics of DOM degradation using three different filtering strategies: 0.2 μm (filtered-and-inoculated), 0.7 μm (generally the most common DOM filter size) and 106 μm ("unfiltered"). We found that bacterial community composition, based on 16S rRNA amplicon sequencing, was significantly affected by the different filter sizes. At the same time, filtering strategy also affected the DOM degradation dynamics. However, the dynamics of these two responses were decoupled, suggesting that filtration primarily influences biolability assays through bacterial abundance and the presence of their associated predators. By the end of the 41-day incubations all treatments tended to converge on a common total DOM biolability level, with the 0.7 μm filtered incubations reaching this point the quickest. These results suggest that assays to assess the total biolability of aquatic DOM should last long enough to remove filtration artefacts in the microbial population. Filtering strategy should also be taken into account when comparing results across biolability assays.

scholarly journals Filtration artefacts in bacterial community composition can affect the outcome of dissolved organic matter biolability assays

2018 ◽  
Vol 15 (23) ◽  
pp. 7141-7154 ◽  
Author(s):  
Joshua F. Dean ◽  
Jurgen R. van Hal ◽  
A. Johannes Dolman ◽  
Rien Aerts ◽  
James T. Weedon
Keyword(s):  
Organic Matter ◽  
Bacterial Community ◽  
Dissolved Organic Matter ◽  
Community Composition ◽  
Bacterial Community Composition ◽  
Amplicon Sequencing ◽  
Microbial Decomposition ◽  
16S Rrna Amplicon Sequencing ◽  
Filter Size ◽  
Carbon Dioxide Co2

Abstract. Inland waters are large contributors to global carbon dioxide (CO2) emissions, in part due to the vulnerability of dissolved organic matter (DOM) to microbial decomposition and respiration to CO2 during transport through aquatic systems. To assess the degree of this vulnerability, aquatic DOM is often incubated in standardized biolability assays. These assays isolate the dissolved fraction of aquatic OM by size filtration prior to incubation. We test whether this size selection has an impact on the bacterial community composition and the consequent dynamics of DOM degradation using three different filtration strategies: 0.2 µm (filtered and inoculated), 0.7 µm (generally the most common DOM filter size) and 106 µm (unfiltered). We found that bacterial community composition, based on 16S rRNA amplicon sequencing, was significantly affected by the different filter sizes. At the same time, the filtration strategy also affected the DOM degradation dynamics, including the δ13C signature. However, the dynamics of these two responses were decoupled, suggesting that filtration primarily influences biolability assays through bacterial abundance and the presence of their associated predators. By the end of the 41-day incubations all treatments tended to converge on a common total DOM biolability level, with the 0.7 µm filtered incubations reaching this point the quickest. These results suggest that assays used to assess the total biolability of aquatic DOM should last long enough to remove filtration artefacts in the microbial population. Filtration strategy should also be taken into account when comparing results across biolability assays.

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