scholarly journals Comparative Analysis of Chemical Constituents of Moringa oleifera Leaves from China and India by Ultra-Performance Liquid Chromatography Coupled with Quadrupole-Time-Of-Flight Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 942 ◽  
Author(s):  
Hongqiang Lin ◽  
Hailin Zhu ◽  
Jing Tan ◽  
Han Wang ◽  
Zhongyao Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Moringa Oleifera ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Multivariate Statistical ◽  
Flight Mass Spectrometry ◽  
Moringa Oleifera Leaves ◽  
Potential Biomarkers

With the aim to discuss the similarities and differences of phytochemicals in Moringa oleifera leaves collected from China (CML) and India (IML) in mind, comparative ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS) analysis was performed in this study. A screening analysis based on a UNIFI platform was first carried out to discuss the similarities. Next, untargeted metabolomic analysis based on multivariate statistical analysis was performed to discover the differences. As a result, a total of 122 components, containing 118 shared constituents, were characterized from CML and IML. The structure types included flavonoids, alkaloids, glyosides, organic acids and organic acid esters, iridoids, lignans, and steroids, etc. For CML, 121 compounds were characterized; among these, 18 potential biomarkers with higher contents enabled differentiation from IML. For IML, 119 compounds were characterized; among these, 12 potential biomarkers with higher contents enabled differentiation from CML. It could be concluded that both CML and IML are rich in phytochemicals and that CML is similar to IML in the kinds of the compounds it contains, except for the significant differences in the contents of some compounds. This comprehensive phytochemical profile study provides a basis for explaining the effect of different growth environments on secondary metabolites and exists as a reference for further research into or applications of CML in China.

scholarly journals Metabolic profiling putatively identifies plasma biomarkers of male infertility using UPLC-ESI-IT-TOFMS

RSC Advances ◽  
2018 ◽  
Vol 8 (46) ◽  
pp. 25974-25982 ◽  
Author(s):  
F. J. Zeng ◽  
H. C. Ji ◽  
Z. M. Zhang ◽  
J. K. Luo ◽  
H. M. Lu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Male Infertility ◽  
Electrospray Ionization ◽  
Metabolic Profiling ◽  
Ion Trap ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Flight Mass Spectrometry ◽  
Potential Biomarkers

Ultra-performance liquid chromatography-electrospray ionization-ion trap-time of flight mass spectrometry combined with chemometrics methods was used to discover potential biomarkers of male infertility based on untargeted plasma metabolomics.

scholarly journals Rapid Evaluation of Chemical Consistency of Artificially Induced and Natural Resina Draconis Using Ultra-Performance Liquid Chromatography Quadrupole-Time-of-Flight Mass Spectrometry-Based Chemical Profiling

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1850 ◽  
Author(s):  
Qianping Chen ◽  
Lili He ◽  
Changming Mo ◽  
Zhifeng Zhang ◽  
Hairong Long ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Market Demand ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry ◽  
Chromatographic Peaks ◽  
Resina Draconis

Resina Draconis is a highly valued traditional medicine widely used in Arabia since ancient times, and it has been commonly used as an antidiarrheic, antimicrobial, antiulcer, blood circulation promoter as well as an anti-inflammatory agent. The tree source from which this medicine orignates grows extremely slowly, producing a very low yield of Resina Draconis. To meet the increasing market demand, artificial methods for stimulating Resina Draconis formation have been developed and applied. However, the chemical differences between artificially induced Resina Draconis (AIRD) and natural Resina Draconis (NRD) have been rarely studied. The aim of this research was to explore and identify the chemical constituents of AIRD and NRD using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS) based chemical profiling. A total of 56 chromatographic peaks were detected in AIRD, of these, 44 peaks have had their structures tentatively characterized based on high-resolution mass spectra (HRMS) data, fragmentation ions information, reference standards data and literature review. In total, 40 peaks were found both in AIRD and NRD. The potential chemical transformation mechanisms active in Resina Draconis during formation were explored. To the best of our knowledge, this is the first evaluation of the chemical profiles of both AIRD and NRD. Furthermore, these findings are expected to provide a rational basis for the quality assessment of AIRD and the use of AIRD as a substitute for NRD.

Chemical profiling of Di-Wu-Yang-Gan Granules by ultra performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry with MSE technology

2018 ◽  
Vol 73 (3-4) ◽  
pp. 107-116
Author(s):  
Dong Tian ◽  
Chao Zhou ◽  
Hong-Mei Jia ◽  
Meng Yu ◽  
Xing Chang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Herbal Medicines ◽  
Ultra Performance Liquid Chromatography ◽  
Chinese Herbs ◽  
Chemical Information ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry

AbstractDi-Wu-Yang-Gan Granules is a Traditional Chinese Medicine prescription used for the treatment of HBeAg-negative chronic hepatitis B patients in China. It consists of five commonly used Chinese herbs. However, the chemical constituents of the whole prescription had not been clarified yet. Hence, in this study, the chemical profiling of Di-Wu-Yang-Gan Granules was explored by ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry, which can provide accurate molecular weight within 5-ppm error and sufficient MS/MS fragment ions without the need for precursor ion selection. As a result, 116 compounds were identified, including lignans, triterpenesaponins, flavonoids, coumarins, iridoids, nortriterpenoids, phenolic acids, and sesquiterpenes. All compounds were further assigned to the individual herbs. In conclusion, this established method was reliable and effective for the separation and identification of the constituents in Di-Wu-Yang-Gan Granules. The findings are beneficial for quality control of the prescription during production and provide helpful chemical information for exploring its efficacy and the mechanism of action. The fragmentation regularity summarized in this study also provided important information for the rapid identification of the chemical composition in herbal medicines or their prescription.

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