The Prevalence and Genotypic Distribution of Group A Rotavirus Detected from Patients with Acute Gastroenteritis Patients in Incheon

Group A rotavirus (RVA) infection is the leading cause of hospitalization of children under 5 years old, presenting with symptoms of acute gastroenteritis. The aim of our study was to explore the genetic diversity of RVA among patients admitted to Moscow Infectious Disease Clinical Hospital No. 1 with symptoms of acute gastroenteritis. A total of 653 samples were collected from May 2019 through March 2020. Out of them, 135 (20.67%) fecal samples were found to be positive for rotavirus antigen by ELISA. RT-PCR detected rotavirus RNA in 80 samples. Seven G-genotypes (G1, G2, G3, G4, G8, G9, and G12) and three P-genotypes (P[8], P[4], and P[6]) formed 9 different combinations. The most common combination was G9P[8]. However, for the first time in Moscow, the combination G3P[8] took second place. Moreover, all detected viruses of this combination belonged to Equine-like G3P[8] viruses that had never been detected in Russia before. The genotype G8P[8] and G9P[4] rotaviruses were also detected in Moscow for the first time. Among the studied rotaviruses, there were equal proportions of Wa and DS-1-like strains; previous studies showed that Wa-like strains accounted for the largest proportion of rotaviruses in Russia.

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Genetic characterization of group A rotavirus strains circulating among children with acute gastroenteritis in Japan in 2004–2005

Infection Genetics and Evolution ◽

10.1016/j.meegid.2006.10.002 ◽

2007 ◽

Vol 7 (2) ◽

pp. 247-253 ◽

Cited By ~ 12

Author(s):

Tung Gia Phan ◽

Pattara Khamrin ◽

Trinh Duy Quang ◽

Shuvra Kanti Dey ◽

Fumihiro Yagyu ◽

...

Keyword(s):

Acute Gastroenteritis ◽

Genetic Characterization ◽

Group A Rotavirus ◽

Group A

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Detection and Genetic Characterization of Group A Rotavirus Strains Circulating among Children with Acute Gastroenteritis in Japan

Journal of Virology ◽

10.1128/jvi.02342-06 ◽

2007 ◽

Vol 81 (9) ◽

pp. 4645-4653 ◽

Cited By ~ 59

Author(s):

Tung Gia Phan ◽

Pattara Khamrin ◽

Trinh Duy Quang ◽

Shuvra Kanti Dey ◽

Sayaka Takanashi ◽

...

Keyword(s):

Amino Acid ◽

Acute Gastroenteritis ◽

Pediatric Population ◽

Amino Acid Substitutions ◽

Predominant Genotype ◽

Group A Rotavirus ◽

Reverse Transcription Pcr ◽

Wide Range ◽

Group A

ABSTRACT A total of 603 fecal specimens collected from July 2005 to June 2006 from children with acute gastroenteritis, encompassing five different localities in Japan, were screened for group A rotavirus by reverse transcription-PCR. It was found that 117 fecal specimens (19.4%) were positive for group A rotavirus. Rotavirus infection was detected continuously from November to June, with the highest prevalence in April. The G (VP7 genotypes) and P (VP4 genotypes) types were further investigated. The predominant genotype was G1P[8] (70.1%), followed by G3P[8] (17.9%), G9P[8] (6.8%), and G2P[4] (2.6%). A number of unusual G1P[4] combinations were also detected during this study period. A novel nomenclature for G1 is proposed, in which worldwide rotavirus G1 strains are classified into 11 lineages with 17 sublineages. A wide range of amino acid substitutions (up to 34) in VP7 that are specific for G1 lineages and sublineages were identified. Interestingly, only short amino acid motifs located at positions 29 to 75 and 211 to 213 of VP7 defined correctly the phylogenetic G1 lineages and sublineages. Examination of the deduced sequences of antigenic regions of VP7 also revealed multiple particular amino acid substitutions that correlated with the phylogenetic G1 lineages and sublineages. Of note, at least three distinct clusters of rotavirus G1 isolates were cocirculating in the Japanese pediatric population studied.

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Diagnostic Accuracy of Seven Commercial Assays for Rapid Detection of Group A Rotavirus Antigens

Journal of Clinical Microbiology ◽

10.1128/jcm.01984-15 ◽

2015 ◽

Vol 53 (11) ◽

pp. 3670-3673 ◽

Cited By ~ 15

Author(s):

Jérôme Kaplon ◽

Céline Fremy ◽

Sylvie Pillet ◽

Lucile Mendes Martins ◽

Katia Ambert-Balay ◽

...

Keyword(s):

Diagnostic Accuracy ◽

Real Time ◽

Reverse Transcription ◽

Acute Gastroenteritis ◽

Rapid Detection ◽

Group A Rotavirus ◽

Reverse Transcription Pcr ◽

Group A ◽

Stool Samples ◽

Human Stool

Seven commercial immunochromatographic assays intended for the detection of group A rotavirus antigens in human stool samples were evaluated. These assays showed similar levels of diagnostic accuracy and were suitable for the detection of rotavirus in patients with acute gastroenteritis but missed some asymptomatic rotavirus shedding identified by real-time reverse transcription-PCR.

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Genome Sequence Analysis of a G2P[4] Group A Rotavirus Strain with an Unusual Canine-Origin NSP1 A15 Genotype

Genome Announcements ◽

10.1128/genomea.01315-17 ◽

2017 ◽

Vol 5 (50) ◽

Author(s):

Chung-Chan Lee ◽

Shih-Yen Chen ◽

Chi-Neu Tsai ◽

Cheng-Hsun Chiu

Keyword(s):

Sequence Analysis ◽

Genome Sequence ◽

Acute Gastroenteritis ◽

Genomic Sequence ◽

Fecal Specimen ◽

Complete Genomic Sequence ◽

Genome Sequence Analysis ◽

Group A Rotavirus ◽

Group A ◽

Complete Genomic

ABSTRACT Here, we disclose the complete genomic sequence of a rare rotavirus group A G2P[4]-I2-R2-C2-M2-A15-N2-T2-E2-H2 strain detected in a fecal specimen from a rotaviral acute gastroenteritis patient who had previously received the Rotarix vaccine.

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Epidemiology of Group A Rotavirus Diarrhea among Children Hospitalized for Acute Gastroenteritis in Ondo State, Nigeria

Journal of Child Science ◽

10.1055/s-0040-1718543 ◽

2021 ◽

Vol 11 (01) ◽

pp. e338-e349

Author(s):

Michael Oluyemi Babalola ◽

David Olufemi Olaleye ◽

Georgina Njideka Odaibo

Keyword(s):

Acute Gastroenteritis ◽

Sub Saharan Africa ◽

Enteric Infection ◽

Chi Square ◽

Female Ratio ◽

Group A Rotavirus ◽

Significant Difference ◽

Group A ◽

Ondo State ◽

Stool Samples

AbstractGlobally, infective group A rotavirus (RVA) enteric infection in children culminates in acute diarrheal disease, severe dehydration, and mortality in children under the age of 5 years, particularly in sub-Saharan Africa. This research sought to determine the prevalence of RVA diarrhea among children aged below 5 years in Ondo state, as one of the necessary frameworks before instituting a vaccine campaign, and to expand knowledge on the epidemiology of RVA diarrhea in Nigeria.In a cross-sectional descriptive study between October 2012 and September 2014, convenience sampling was adopted to obtain demographic information, clinical details, and stool samples from accented under five children who sought treatment for acute gastroenteritis at designated hospitals in Akure and Owo, Nigeria. A total of 390 stool samples were collected from children with acute diarrhea and tested for VP6 RVA antigen using enzyme immunoassay. Data generated were analyzed using descriptive statistics and chi-square at α 0.05.From the 390 children hospitalized for diarrhea, 240 samples (240/390; 61.5%) were from males, while 150 samples (150/390; 38.5%) were from females, representing a male:female ratio of 1.6:1. RVAs were found in 24.2% (58/240) males and 28% (42/150) females, giving a male-female ratio of 1:1.2 and total prevalence of 25.6% (100/390). RVA infection was inversely proportional to the age as a rate of 11.8% was observed in children aged above 36 months and 31.8% (35/110) in children 7 to 12 months, while the highest rate (45.7%) was among children ≤ 6 months old. No significant difference was found (chi-square = 0.712) in the induction of diarrhea in children from Akure and Owo, neither was there any significant difference in the rates of infection between the boys and girls in Akure (chi-square = 0.576) nor in Owo (chi-square = 0.333). Seasonal association (chi-square = 5.802) in RVA infection was observed in the rainy season of year 2013/2014 period.RVA diarrhea occurred year-round at a prevalence of 25.6%, predominantly in females. A seasonal fluctuation was observed in the rainy and dry seasons of the 2-year period. RVA diarrhea occurred predominantly in children aged below 18 months of age, and may thus help in determining the optimal period/schedule of any immunization program.

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