scholarly journals Iron Oxide Nanoparticle Uptake in Mouse Brachiocephalic Artery Atherosclerotic Plaque Quantified by T2-Mapping MRI

Pharmaceutics ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 279
Author(s):  
Rik P. M. Moonen ◽  
Bram F. Coolen ◽  
Judith C. Sluimer ◽  
Mat J. A. P. Daemen ◽  
Gustav J. Strijkers
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Iron Oxide ◽  
Atherosclerotic Plaque ◽  
Ex Vivo ◽  
T2 Mapping ◽  
Atherosclerotic Plaques ◽  
Brachiocephalic Artery ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

The purpose of our study was to monitor the iron oxide contrast agent uptake in mouse brachiocephalic artery (BCA) atherosclerotic plaques in vivo by quantitative T2-mapping magnetic resonance imaging (MRI). Female ApoE−/− mice (n = 32) on a 15-week Western-type diet developed advanced plaques in the BCA and were injected with ultra-small superparamagnetic iron oxides (USPIOs). Quantitative in vivo MRI at 9.4 T was performed with a Malcolm-Levitt (MLEV) prepared T2-mapping sequence to monitor the nanoparticle uptake in the atherosclerotic plaque. Ex vivo histology and particle electron paramagnetic resonance (pEPR) were used for validation. Longitudinal high-resolution in vivo T2-value maps were acquired with consistent quality. Average T2 values in the plaque decreased from a baseline value of 34.5 ± 0.6 ms to 24.0 ± 0.4 ms one day after injection and partially recovered to an average T2 of 27 ± 0.5 ms after two days. T2 values were inversely related to iron levels in the plaque as determined by ex vivo particle electron paramagnetic resonance (pEPR). We concluded that MRI T2 mapping facilitates a robust quantitative readout for USPIO uptake in atherosclerotic plaques in arteries near the mouse heart.

scholarly journals The Contribution of Electron Paramagnetic Resonance to Melanoma Research

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Quentin Godechal ◽  
Bernard Gallez
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Skin Cancer ◽  
Ex Vivo ◽  
Current Method ◽  
Basic Knowledge ◽  
Ultraviolet Rays ◽  
Paramagnetic Resonance ◽  
Endogenous Substrate ◽  
Electron Paramagnetic

The incidence of malignant melanoma, the most dangerous form of skin cancer, is rising each year. However, some aspects of the tumor initiation and development are still unclear, and the current method of diagnosis, based on the visual aspect of the tumor, shows limitations. For these reasons, developments of new techniques are ongoing to improve basic knowledge on the disease and diagnosis of tumors in individual patients. This paper shows how electron paramagnetic resonance (EPR), a method able to detect free radicals trapped in melanin pigments, has recently brought its unique value to this specific field. The general principles of the method and the convenience of melanin as an endogenous substrate for EPR measurements are explained. Then, the way by which EPR has recently helped to assess the contribution of ultraviolet rays (UVA and UVB) to the initiation of melanoma is described. Finally, we describe the improvements of EPR spectrometry and imaging in the detection and mapping of melanin pigments insideex vivoandin vivomelanomas. We discuss how these advances might improve the diagnosis of this skin cancer and point out the present capabilities and limitations of the method.

In vivo visualization and ex vivo quantification of murine breast cancer cells in the mouse brain using MRI cell tracking and electron paramagnetic resonance

2015 ◽  
Vol 28 (3) ◽  
pp. 367-375 ◽  
Author(s):  
Pierre Danhier ◽  
Julie Magat ◽  
Philippe Levêque ◽  
Géraldine De Preter ◽  
Paolo E. Porporato ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Electron Paramagnetic Resonance ◽  
Cancer Cells ◽  
Mouse Brain ◽  
Breast Cancer Cells ◽  
Cell Tracking ◽  
Ex Vivo ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

ДИНИТРОЗИЛЬНЫЕ КОМПЛЕКСЫ ЖЕЛЕЗА C ТИОЛСОДЕРЖАЩИМИ ЛИГАНДАМИ ПРЕДСТАВЛЕНЫ В ЖИВЫХ ОРГАНИЗМАХ В ОСНОВНОМ ИХ БИЯДЕРНОЙ ФОРМОЙ

2020 ◽  
Vol 65 (6) ◽  
pp. 1142-1153
Author(s):  
В.Д. Микоян ◽  
◽  
Е.Н. Бургова ◽  
Р.Р. Бородулин ◽  
А.Ф. Ванин ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Sodium Nitrite ◽  
Iron Complexes ◽  
Paramagnetic Resonance ◽  
First Case ◽  
Dinitrosyl Iron ◽  
Electron Paramagnetic ◽  
Dinitrosyl Complexes

The number of mononitrosyl iron complexes with diethyldithiocarbamate, formed in the liver of mice in vivo and in vitro after intraperitoneal injection of binuclear dinitrosyl iron complexes with N-acetyl-L-cysteine or glutathione, S-nitrosoglutathione, sodium nitrite or the vasodilating drug Isoket® was assessed by electron paramagnetic resonance (EPR). The number of the said complexes, in contrast to the complexes, formed after nitrite or Isoket administration, the level of which sharply increased after treatment of liver preparations with a strong reducing agent - dithionite, did not change in the presence of dithionite. It was concluded that, in the first case, EPR-detectable mononitrosyl iron complexes with diethyldithiocarbamate in the absence and presence of dithionite appeared as a result of the reaction of NO formed from nitrite with Fe2+-dieth- yldithiocarbamate and Fe3+-diethyldithiocarbamate complexes, respectively. In the second case, mononitrosyl iron complexes with diethyldithiocarbamate appeared as a result of the transition of iron-mononitosyl fragments from ready-made iron-dinitrosyl groups of binuclear dinitrosyl complexes, which is three to four times higher than the content of the mononuclear form of these complexes in the tissue...

Evaluation of adriamycin nephropathy by an in vivo electron paramagnetic resonance

2005 ◽  
Vol 332 (2) ◽  
pp. 326-331 ◽  
Author(s):  
Takaaki Oteki ◽  
Sohji Nagase ◽  
Hidekatsu Yokoyama ◽  
Hiroaki Ohya ◽  
Takao Akatsuka ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Paramagnetic Resonance ◽  
Adriamycin Nephropathy ◽  
Electron Paramagnetic
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