Candidate Genes and Quantitative Trait Loci for Grain Yield and Seed Size in Durum Wheat

Grain yield (YLD) is affected by thousand kernel weight (TKW) which reflects the combination of grain length (GL), grain width (GW) and grain area (AREA). Grain weight is also influenced by heading time (HT) and plant height (PH). To detect candidate genes and quantitative trait loci (QTL) of yield components, a durum wheat recombinant inbred line (RIL) population was evaluated in three field trials. The RIL was genotyped with a 90K single nucleotide polymorphism (SNP) array and a high-density genetic linkage map with 5134 markers was obtained. A total of 30 QTL were detected including 23 QTL grouped in clusters on 1B, 2A, 3A, 4B and 6B chromosomes. A QTL cluster on 2A chromosome included a major QTL for HT co-located with QTL for YLD, TKW, GL, GW and AREA, respectively. The photoperiod sensitivity (Ppd-A1) gene was found in the physical position of this cluster. Serine carboxypeptidase, Big grain 1 and β-fructofuranosidase candidate genes were mapped in clusters containing QTL for seed size. This study showed that yield components and phenological traits had higher inheritances than grain yield, allowing an accurate QTL cluster detection. This was a requisite to physically map QTL on durum genome and to identify candidate genes affecting grain yield.

Download Full-text

Candidate Genes and Stable QTL for Grain Yield and Seed Size in Durum Wheat

10.21203/rs.3.rs-34145/v3 ◽

2021 ◽

Author(s):

Giacomo Mangini ◽

Antonio Blanco ◽

Domenica Nigro ◽

Massimo Antonio Signorile ◽

Rosanna Simeone

Keyword(s):

Grain Yield ◽

Durum Wheat ◽

Candidate Genes ◽

Yield Components ◽

Snp Array ◽

Phenotypic Variance ◽

Serine Carboxypeptidase ◽

Growing Seasons ◽

Qtl Cluster ◽

Ril Population

Abstract Background: In wheat grain yield is expressed as the product of different components. Among these, thousand kernels weight (TKW) reflects the combination of several grain related traits including grain length (GL), grain width (GW) and area. Grain weight is also affected by phenological traits, such as heading time (HT) and plant height (PH). To detect stable QTL and candidate genes involved in phenotypic control of grain yield, a recombinant inbred line (RIL) population derived from two elite durum wheat cultivars (Liberdur and Anco Marzio) was evaluated for yield components and grain related traits for three growing seasons in southern Italy. The mapping population was genotyped with a 90K SNP array and a high-density genetic linkage map with 5134 markers was obtained.Results: A total of 30 QTL were detected on the durum RIL population including 9 stable QTL for TKW (2 QTL), GL, GW (2 QTL), AREA, HT and PH (2 QTL) distributed on 1B, 2A, 3A and 6B chromosomes. Interestingly, a QTL cluster mapped on 2A included a major QTL for HT explaining at least 70% of phenotypic variance and co-located with a QTL for YLD, TKW, GL and GW and AREA, respectively. In the physical position of this QTL cluster a photoperiod sensitivity gene (Ppd-A1) was found. Serine carboxypeptidase, Big Grain 1 and β-fructofuranosidase candidate genes were mapped in clusters containing stable QTL. Candidate genes involved in auxin metabolism were also found in QTL clusters in which a QTL for AREA was declared. Conclusions: This study showed that yield components and phenological traits had higher inheritances than grain yield, allowing an accurate stable QTL cluster detection. This was a powerful requisite to physically map QTL on the reference durum wheat genome and to identify candidate genes strongly affecting the genetic grain yield network.

Download Full-text

Candidate Genes and Stable QTL for Grain Yield and Seed Size in Durum Wheat

10.21203/rs.3.rs-34145/v2 ◽

2020 ◽

Author(s):

Giacomo Mangini ◽

Antonio Blanco ◽

Domenica Nigro ◽

Massimo Antonio Signorile ◽

Rosanna Simeone

Keyword(s):

Grain Yield ◽

Durum Wheat ◽

Candidate Genes ◽

Yield Components ◽

Snp Array ◽

Phenotypic Variance ◽

Serine Carboxypeptidase ◽

Growing Seasons ◽

Qtl Cluster ◽

Ril Population

Abstract Background: In wheat grain yield is expressed as the product of different components. Among these, thousand kernels weight (TKW) reflects the combination of several grain related traits including grain length (GL), grain width (GW) and area. Grain weight is also affected by phenological traits, such as heading time (HT) and plant height (PH). To detect stable QTL and candidate genes involved in phenotypic control of grain yield, a recombinant inbred line (RIL) population derived from two elite durum wheat cultivars (Liberdur and Anco Marzio) was evaluated for yield components and grain related traits for three growing seasons in southern Italy. The mapping population was genotyped with a 90K SNP array and a high-density genetic linkage map with 5134 markers was obtained.Results: A total of 30 QTL were detected on the durum RIL population including 9 stable QTL for TKW (2 QTL), GL, GW (2 QTL), AREA, HT and PH (2 QTL) distributed on 1B, 2A, 3A and 6B chromosomes. Interestingly, a QTL cluster mapped on 2A included a major QTL for HT explaining at least 70% of phenotypic variance and co-located with a QTL for YLD, TKW, GL and GW and AREA, respectively. In the physical position of this QTL cluster a photoperiod sensitivity gene (Ppd-A1) was found. Serine carboxypeptidase, Big Grain 1 and β-fructofuranosidase candidate genes were mapped in clusters containing stable QTL. Candidate genes involved in auxin metabolism were also found in QTL clusters in which a QTL for AREA was declared.Conclusions: This study showed that yield components and phenological traits had higher inheritances than grain yield, allowing an accurate stable QTL cluster detection. This was a powerful requisite to physically map QTL on the reference durum wheat genome and to identify candidate genes strongly affecting the genetic grain yield network.

Download Full-text

Detection of Quantitative Trait Loci for Grain Yield and Yield Components Across Environments in Durum Wheat

Cereal Research Communications ◽

10.1007/bf03543666 ◽

2001 ◽

Vol 29 (3-4) ◽

pp. 237-244 ◽

Cited By ~ 5

Author(s):

A. Blanco ◽

C. Lotti ◽

R. Simeone ◽

A. Signorile ◽

V. De Santis ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Grain Yield ◽

Durum Wheat ◽

Quantitative Trait ◽

Yield Components ◽

Yield And Yield Components ◽

Trait Loci

Download Full-text

Stable Qtl And Candidate Genes Involved Into The Genetic Network Affecting Grain Yield And Seed Size In Durum Wheat

10.21203/rs.3.rs-34145/v1 ◽

2020 ◽

Author(s):

Giacomo Mangini ◽

Antonio Blanco ◽

Domenica Nigro ◽

Massimo Antonio Signorile ◽

Rosanna Simeone

Keyword(s):

Grain Yield ◽

Durum Wheat ◽

Candidate Genes ◽

Yield Components ◽

Snp Array ◽

Genetic Network ◽

Phenotypic Variance ◽

Serine Carboxypeptidase ◽

Qtl Cluster ◽

Ril Population

Abstract Background: In wheat grain yield is expressed as the product of different components. Among these, thousand kernels weight (TKW) reflects the combination of several grain related traits including grain length (GL), grain width (GW) and area. Grain weight is also affected by phenological traits, such as heading time (HT) and plant height (PH). To detect stable QTL and candidate genes involved in phenotypic control of grain yield, a recombinant inbred line (RIL) population derived from two elite durum wheat cultivars (Liberdur and Anco Marzio) was evaluated for yield components and grain related traits for three growing seasons in southern Italy. The mapping population was genotyped with a 90K SNP array and a high-density genetic linkage map with 5134 markers were obtained.Results: A total of 30 QTL were detected on the durum RIL population including 9 stable QTL for TKW (2 QTL), GL, GW (2 QTL), AREA, HT and PH (2 QTL) distributed on 1B, 2A, 3A and 6B chromosomes. Interestingly, a QTL cluster mapped on 2A included a major QTL for HT explaining at least 70% of phenotypic variance and co-located with a QTL for YLD, TKW, GL and GW and AREA, respectively. In the physical position of this QTL cluster a photoperiod sensitivity gene (Ppd-A1) was found. Serine carboxypeptidase, Big Grain 1 and β-fructofuranosidase candidate genes were mapped in clusters containing stable QTL. Candidate genes involved in auxin metabolism were also found in QTL clusters in which a QTL for AREA was declared. Conclusions: This study showed that yield components and phenological traits had higher inheritances than grain yield, allowing an accurate stable QTL cluster detection. This was a powerful requisite to physically map QTL on the reference durum wheat genome and to identify candidate genes strongly affecting the genetic grain yield network.

Download Full-text