The Copper Amine Oxidase AtCuAOδ Participates in Abscisic Acid-Induced Stomatal Closure in Arabidopsis

Plant copper amine oxidases (CuAOs) are involved in wound healing, defense against pathogens, methyl-jasmonate-induced protoxylem differentiation, and abscisic acid (ABA)-induced stomatal closure. In the present study, we investigated the role of the Arabidopsis thaliana CuAOδ (AtCuAOδ; At4g12290) in the ABA-mediated stomatal closure by genetic and pharmacological approaches. Obtained data show that AtCuAOδ is up-regulated by ABA and that two Atcuaoδ T-DNA insertional mutants are less responsive to this hormone, showing reduced ABA-mediated stomatal closure and H2O2 accumulation in guard cells as compared to the wild-type (WT) plants. Furthermore, CuAO inhibitors, as well as the hydrogen peroxide (H2O2) scavenger N,N1-dimethylthiourea, reversed most of the ABA-induced stomatal closure in WT plants. Consistently, AtCuAOδ over-expressing transgenic plants display a constitutively increased stomatal closure and increased H2O2 production compared to WT plants. Our data suggest that AtCuAOδ is involved in the H2O2 production related to ABA-induced stomatal closure.

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Unique protonation states of aspartate and topaquinone in the active site of copper amine oxidase

RSC Advances ◽

10.1039/d0ra06365g ◽

2020 ◽

Vol 10 (63) ◽

pp. 38631-38639

Author(s):

Mitsuo Shoji ◽

Takeshi Murakawa ◽

Mauro Boero ◽

Yasuteru Shigeta ◽

Hideyuki Hayashi ◽

...

Keyword(s):

Biogenic Amines ◽

Active Site ◽

Amine Oxidase ◽

First Principle ◽

Amine Oxidases ◽

Oxidative Deamination ◽

Copper Amine Oxidase ◽

First Principle Calculations ◽

Copper Amine Oxidases ◽

Copper Amine

Copper amine oxidases catalyze the oxidative deamination of biogenic amines. We investigated the unique protonation states in the active site using first-principle calculations.

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Copper amine oxidase and phospholipase D act independently in abscisic acid (ABA)-induced stomatal closure in Vicia faba and Arabidopsis

Journal of Plant Research ◽

10.1007/s10265-014-0633-3 ◽

2014 ◽

Vol 127 (4) ◽

pp. 533-544 ◽

Cited By ~ 17

Author(s):

Yana Qu ◽

Zhenfeng An ◽

Baocheng Zhuang ◽

Wen Jing ◽

Qun Zhang ◽

...

Keyword(s):

Abscisic Acid ◽

Vicia Faba ◽

Phospholipase D ◽

Amine Oxidase ◽

Stomatal Closure ◽

Copper Amine Oxidase ◽

Copper Amine

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Copper amine oxidase-catalysed hydrogen peroxide involves production of nitric oxide in darkness-induced stomatal closure in broad bean

Functional Plant Biology ◽

10.1071/fp15172 ◽

2015 ◽

Vol 42 (11) ◽

pp. 1057 ◽

Cited By ~ 5

Author(s):

Ai-Xia Huang ◽

Yong-Shun Wang ◽

Xiao-Ping She ◽

Juan Mu ◽

Jin-Liang Zhao

Keyword(s):

Nitric Oxide ◽

Hydrogen Peroxide ◽

Amine Oxidase ◽

Stomatal Closure ◽

H2o2 Production ◽

No Production ◽

Copper Amine Oxidase ◽

Irreversible Inhibitors ◽

Important Intermediate ◽

Copper Amine

Hydrogen peroxide is an important intermediate in darkness-induced stomatal closure. In the present work, we provide evidence that copper amine oxidase (CuAO) was involved in H2O2 production in darkness-induced stomatal closure in Vicia faba L. Darkness activated CuAO in intercellular washing fluid from leaves. Aminoguanidine (AG) and 2-bromoethylamine (BEA), which were both irreversible inhibitors of CuAO, significantly suppressed darkness-induced stomatal closure and H2O2 generation. The effects of AG and BEA were reversed only by H2O2 but not by other products of CuAO. These results indicate that CuAO participates in darkness-induced stomatal closure through its reaction product, H2O2. Furthermore, darkness-induced nitric oxide (NO) production and cytosolic alkalinisation were obviously inhibited by AG and BEA, and only H2O2, among the products of CuAO, could reverse the effects, implying that the CuAO-catalysed product H2O2 is required for NO production and cytosolic alkalinisation to a large extent in darkness-induced stomatal closure. In addition, butyric acid blocked but methylamine enhanced the ability of H2O2 to reverse the effect of BEA on NO production, suggesting that cytosolic alkalinisation is involved in CuAO-mediated NO generation in darkness-induced stomatal closure.

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