scholarly journals Molecular Identification and VOMs Characterization of Saccharomyces cerevisiae Strains Isolated from Madeira Region Winery Environments

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1058 ◽  
Author(s):  
Mariangie Castillo ◽  
Emanuel da Silva ◽  
José S. Câmara ◽  
Mahnaz Khadem
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Solid Phase ◽  
Rflp Analysis ◽  
Selective Adaptation ◽  
Internal Transcribed Spacers ◽  
Gas Chromatography Mass Spectrometry ◽  
Pcr Rflp ◽  
Volatile Organic ◽  
Major Chemical

The quality and typical characteristic of wines depends, among other factors, on the volatile organic metabolites (VOMs) that are biosynthesized by yeasts, mainly Saccharomyces cerevisiae species. The yeast strain influences the diversity and proportions of the VOMs produced during the fermentation process, as the genetic predisposition of the strains is a by-product of selective adaptation to the ecosystem. The present work reports the characterization of S. cerevisiae strains isolated from grape must, used in the Demarcated Region of Madeira (DRM) for winemaking. Yeast species were identified by amplification and by restriction fragment length polymorphism (RFLP) analysis of the region 5.8S-internal transcribed spacers (PCR-RFLP of 5.8S-ITS) of ribosomal DNA (rDNA). The strains identification was performed by analyzing the RFLP pattern of mitochondrial DNA (RFLP-mtDNA). The representative strains were selected for the characterization of the volatile profile through headspace solid-phase microextraction (HS-SPME) followed by gas chromatography-mass spectrometry (GC-MS) analysis. A total of 77 VOMs were identified. Higher alcohols, esters, and fatty acids were the major chemical families representing 63%, 16%, and 9%, respectively, in strain A and 54%, 23%, and 15% in strain B. The results indicate the influence of the strain metabolism in the production of VOMs, many of which probably participate in the aroma of the corresponding wines.

scholarly journals Evaluation of Headspace Solid-Phase Microextraction Gas Chromatography–Mass Spectrometry for the Characterization of Volatile Organic Compounds from Melon (Cucumis melo L.) Flowers

2018 ◽  
Vol 81 (8) ◽  
pp. 1231-1239 ◽  
Author(s):  
Francisca Aliny Nunes Silva ◽  
Alexander Alves da Silva ◽  
Nayanny de Sousa Fernandes ◽  
Tigressa Helena Soares Rodrigues ◽  
Kirley Marques Canuto ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Organic Compounds ◽  
Cucumis Melo ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Organic ◽  
Cucumis Melo L

scholarly journals Method for characterization of volatile organic compounds in the diet of Centris analis (Hymenoptera, Apidae, Centridini) immatures

2020 ◽  
Vol 9 (10) ◽  
pp. e5069108880
Author(s):  
Paulo Herbesson Pereira de Sousa ◽  
Cláudia Inês da Silva ◽  
Breno Magalhães Freitas ◽  
Tigressa Helena Rodrigues Soares ◽  
Isac Gabriel Abrahao Bomfim ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Volatile Compounds ◽  
Organic Compounds ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Lower Number ◽  
Deionized Water ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Organic

This study tested three types of Solid-Phase Microextraction fibers in developing a method to extract volatile organic compounds present in the diet of immature Centris analis. Samples were placed in glass vials with metal lids and added with 3g NaCl and 8 ml deionized water. Extraction and characterization were carried out using a Headspace – Solid Phase Microextraction (HS-SPME) with Gas Chromatography – Mass Spectrometry, and the three types of fibers were polydimethylsiloxane (PDMS), divinylbenzene/ carboxen/ polydimethylsiloxane (DVB/CAR/PDMS) and carboxen/ polydimethylsiloxane (CAR/PDMS). Each type of fiber was exposed to volatiles for 30 min and analyzed in a chromatograph Agilent GC-MS equipped with a quadrupole detector (MSD 5977A), containing a HP-5MS (30 m x 0.25 mm x 0.25 µm) column and Helium as the carrier gas (1 ml.min-1). The CAR / PDMS fiber favored the extraction of volatile compounds to semi-volatile compounds, followed by DVB / CAR / PDMS, while PDMS presented a lower number of extracted compounds, which can be attributed to its apolar nature. The volatile compounds identified in the diet included alcohols, aldehydes, esters, ketones, and terpenes. The SPME technique has proven effective in the extraction of volatile organic compounds from immature of Centris analis diet, being the CAR/PDMS the most suitable fiber for this.

scholarly journals Volatile Profile Characterization of Commercial Peach (Prunus persica) Cultivars Grown in Georgia, USA

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 516
Author(s):  
Jamal Mohammed ◽  
Catherine E. Belisle ◽  
Shangci Wang ◽  
Rachel A. Itle ◽  
Koushik Adhikari ◽  
...  
Keyword(s):  
Solid Phase Microextraction ◽  
Prunus Persica ◽  
Solid Phase ◽  
Average Concentration ◽  
Consumer Perception ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Profile ◽  
Volatile Organic ◽  
Mean Concentration

Peach production in Georgia, USA, extends from mid-May to mid-August. Multiple cultivars are commercially grown in the U.S., and each cultivar has unique fruit quality characteristics, which could influence consumer perception and acceptability. Among those, peach flavor has been minimally characterized among cultivars. Headspace-solid phase microextraction (HS–SPME) combined with gas chromatography–mass spectrometry (GC–MS) was used to characterize the volatile profile of 42 peach cultivars commercially grown in 2016. The goal of this research was to understand the aroma composition and content of peach cultivars grown in Georgia, USA. Thirty-six volatile compounds were identified. Significant differences (p ≤ 0.0001) were observed for all the flavor volatiles across all cultivars being evaluated. Esters were the major compounds isolated from the 42 peach cultivars, followed by lactones, alcohols, aldehydes, and terpenoids, in that order. Lactones, benzaldehyde, and linalool, which are known to be peach aroma compounds, exhibited the highest mean concentrations among the cultivars with ɣ- and δ-decalactones having a concentration of approximately 180 ng·g−1 and 60 ng·g−1, respectively. Among the cultivars, “Majestic” had the highest mean concentration of ɣ-decalactone, followed by “Southern Pearl” with approximately 503 ng·g−1 and 443 ng·g−1, respectively. “Southern Pearl” and “Fairtime” also exhibited the highest average concentration of δ-decalactone. The results obtained will help provide an understanding of the distribution of the various volatile organic compounds found in Georgia-grown peach cultivars.

Untargeted SPME–GC–MS Characterization of VOCs Released from Spray Paint

2020 ◽  
Author(s):  
Mitchell Tiessen ◽  
Naomi L Stock ◽  
Theresa Stotesbury
Keyword(s):  
Aromatic Compounds ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Physical Evidence ◽  
Low Concentrations ◽  
Hydrocarbon Chains ◽  
Volatile Organic ◽  
Time Since Deposition

Abstract Paints are a common form of physical evidence encountered at crime scenes. This research presents an optimized method for the untargeted analysis of volatile organic compounds (VOCs) in spray paint using solid-phase microextraction–gas chromatography–mass spectrometry (SPME–GC–MS). The presence and persistence of VOCs were monitored in 30 minute intervals, over a 4 hour period, in a triplicate time study. As predicted, spray paint solvents are lost to the environment readily, whereas few VOCs remained present in the headspace in low concentrations beyond 4 hours. The VOCs that were observed to have the highest persistence in the headspace were aromatic compounds and those with longer hydrocarbon chains. We present this study in a forensic science context and suggest that the interpretation of the results may be useful for forensic applications in establishing a time since deposition of a spray-painted surface.

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