Sintered Glass Monoliths as Supports for Affinity Columns

A novel stationary phase for affinity separations is presented. This material is based on sintered borosilicate glass readily available as semi-finished filter plates with defined porosity and surface area. The material shows fast binding kinetics and excellent long-term stability under real application conditions due to lacking macropores and high mechanical rigidity. The glass surface can be easily modified with standard organosilane chemistry to immobilize selective binders or other molecules used for biointeraction. In this paper, the manufacturing of the columns and their respective column holders by 3D printing is shown in detail. The model system protein A/IgG was chosen as an example to examine the properties of such monolithic columns under realistic application conditions. Several specifications, such as (dynamic) IgG capacity, pressure stability, long-term performance, productivity, non-specific binding, and peak shape, are presented. It could be shown that due to the very high separation speed, 250 mg antibody per hour and column can be collected, which surpasses the productivity of most standard columns of the same size. The total IgG capacity of the shown columns is around 4 mg (5.5 mg/mL), which is sufficient for most tasks in research laboratories. The cycle time of an IgG separation can be less than 1 min. Due to the glass material’s excellent pressure resistance, these columns are compatible with standard HPLC systems. This is usually not the case with standard affinity columns, limited to manual use or application in low-pressure systems. The use of a standard HPLC system also improves the ability for automation, which enables the purification of hundreds of cell supernatants in one day. The sharp peak shape of the elution leads to an enrichment effect, which might increase the concentration of IgG by a factor of 3. The final concentration of IgG can be around 7.5 mg/mL without the need for an additional nanofiltration step. The purity of the IgG was > 95% in one step and nearly 99% with a second polishing run.

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Sintered Glass Monoliths as New Supports for Affinity Columns

10.20944/preprints202103.0298.v1 ◽

2021 ◽

Author(s):

Marco Wilke ◽

Bettina Röder ◽

Martin Paul ◽

Michael G. Weller

Keyword(s):

Specific Binding ◽

Protein A ◽

Peak Shape ◽

Long Term Stability ◽

Sintered Glass ◽

One Step ◽

Pressure Resistance ◽

Long Term Performance ◽

Term Performance

A novel stationary phase for affinity separations is presented. This material is based on sintered borosilicate glass readily available as semi-finished filter plates with defined porosity and surface area. The material shows fast binding kinetics and excellent long-term stability under real application conditions due to lacking macropores and high mechanical rigidity. The glass surface can be easily modified with standard organosilane chemistry to immobilize selective binders or other molecules used for biointeraction. In this paper, the manufacturing of the columns and their respective column holders by 3D printing is shown in detail. The model system protein A/IgG was chosen as an example to examine the properties of such monolithic columns under realistic application conditions. Several specifications, such as (dynamic) IgG capacity, pressure stability, long-term performance, productivity, non-specific binding, and peak shape, are presented. It could be shown that due to the very high separation speed, 250 mg antibody per hour and column can be collected, which surpasses the productivity of most standard columns of the same size. The total IgG capacity of the shown columns is around 4 mg (5.5 mg/mL), which is sufficient for most tasks in research laboratories. The cycle time of an IgG separation can be less than 1 minute. Due to the glass material's excellent pressure resistance, these columns are compatible with standard HPLC systems. This is usually not the case with standard affinity columns, limited to manual use or application in low-pressure systems. The use of a standard HPLC system also improves the ability for automation, which enables the purification of hundreds of cell supernatants in one day. The sharp peak shape of the elution leads to an enrichment effect, which might increase the concentration of IgG by a factor of 3. The final concentration of IgG can be around 7.5 mg/mL without the need for an additional nanofiltration step. The purity of the IgG was > 95% in one step and nearly 99% with a second polishing run.

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Application of electrochemical methods in the development of models for fuel dissolution and container corrosion under nuclear waste disposal conditions

Canadian Journal of Chemistry ◽

10.1139/v97-188 ◽

1997 ◽

Vol 75 (11) ◽

pp. 1566-1584 ◽

Cited By ~ 7

Author(s):

D.W. Shoesmith ◽

W.H. Hocking ◽

B.M. Ikeda ◽

F. King ◽

J.J. Noël ◽

...

Keyword(s):

Nuclear Fuel ◽

Anodic Dissolution ◽

Nuclear Waste ◽

Chloride Solutions ◽

Long Term Stability ◽

Waste Container ◽

Transport Barriers ◽

Long Term Performance ◽

Term Performance

The permanent disposal of nuclear fuel wastes requires the development of models that can assess the performance of a disposal vault over long periods of time. Models to assess the long-term stability of the nuclear fuel (UO2) and the corrosion performance of the waste container (either copper or titanium) have been based on electrochemical principles. Here we review the chemical/electrochemical performance of fuel and the two candidate container materials, and describe some of the electrochemical studies undertaken either to develop the mechanistic understanding upon which these models are based or to measure the values of parameters required to evaluate long-term performance. These include the following: the anodic dissolution of UO2; the reduction of O2 on various specimens of UO2; the crevice corrosion of various titanium alloys; the impedance characteristics of passive films on Ti alloys; the anodic dissolution of copper in chloride solutions; the reduction of O2 on copper; the effect of various transport barriers on the corrosion of copper; and the prediction of the corrosion potential of copper in aerated chloride solutions. Keywords: uranium dioxide, copper, titanium, nuclear waste, oxygen.

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Long-term stability and reusability of molecularly imprinted polymers

Polymer Chemistry ◽

10.1039/c6py01853j ◽

2017 ◽

Vol 8 (4) ◽

pp. 666-673 ◽

Cited By ~ 79

Author(s):

Jozsef Kupai ◽

Mayamin Razali ◽

Sibel Buyuktiryaki ◽

Rustem Kecili ◽

Gyorgy Szekely

Keyword(s):

Molecularly Imprinted Polymers ◽

Functional Monomer ◽

Molecularly Imprinted ◽

Imprinted Polymers ◽

Adsorption Studies ◽

Term Stability ◽

Long Term Stability ◽

Long Term Performance ◽

Term Performance

The effect of crosslinker, functional monomer and extraction on the long-term performance and degradation of molecularly imprinted polymers was investigated through adsorption studies, NMR, SEM, TGA and BET.

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Building the COllaborative Carbon Column Observing Network (COCCON): Long term stability and ensemble performance of the EM27/SUN Fourier transform spectrometer

10.5194/amt-2018-146 ◽

2018 ◽

Cited By ~ 1

Author(s):

Matthias Frey ◽

Mahesh Kumar Sha ◽

Frank Hase ◽

Matthäus Kiel ◽

Thomas Blumenstock ◽

...

Keyword(s):

Total Carbon ◽

Long Term Stability ◽

Long Term Study ◽

Ensemble Performance ◽

Instrumental Line ◽

Consistent Performance ◽

Institute Of Technology ◽

Long Term Performance ◽

Term Performance

Abstract. In a 3.5 year long study, the long term performance of a mobile Bruker EM27/SUN spectrometer, used for greenhouse gases observations, is checked with respect to a co-located reference Bruker IFS 125HR spectrometer, which is part of the Total Carbon Column Observing Network (TCCON). We find that the EM27/SUN is stable on timescales of several years, qualifying it as an useful supplement for the existing TCCON network in remote areas. For achieving consistent performance, such an extension requires careful testing of any spectrometers involved by application of common quality assurance measures. One major aim of the COllaborative Carbon Column Observing Network (COCCON) infrastructure is to provide these services to all EM27/SUN operators. In the framework of COCCON development, the performance of an ensemble of 30 EM27/SUN spectrometers was tested and found to be very uniform, enhanced by the centralized inspection performed at the Karlsruhe Institute of Technology prior to deployment. Taking into account measured instrumental line shape parameters for each spectrometer, the resulting average bias across the ensemble in XCO2 is 0.20 ppm, while it is 0.8 ppb for XCH4. As indicated by the executed long-term study on one device presented here, the remaining empirical calibration factor deduced for each individual instrument can be assumed constant over time. Therefore the application of these empirical factors is expected to further improve the EM27/SUN network conformity beyond the raw residual bias reported above.

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