scholarly journals Genotoxic Effects of Aluminum Chloride and Their Relationship with N-Nitroso-N-Methylurea (NMU)-Induced Breast Cancer in Sprague Dawley Rats

Toxics ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 31
Author(s):  
Alejandro Monserrat García-Alegría ◽  
Agustín Gómez-Álvarez ◽  
Iván Anduro-Corona ◽  
Armando Burgos-Hernández ◽  
Eduardo Ruíz-Bustos ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Genomic Instability ◽  
Aluminum Chloride ◽  
Dietary Behavior ◽  
Mammary Glands ◽  
Genotoxic Effect ◽  
Aluminum Concentration ◽  
Sprague Dawley ◽  
Experimental Conditions ◽  
Sprague Dawley Rats

Recently, soluble forms of aluminum for human use or consumption have been determined to be potentially toxic due to their association with hepatic, neurological, hematological, neoplastic, and bone conditions. This study aims to assess the genotoxic effect of aluminum chloride on genomic instability associated with the onset of N-nitroso-N-methylurea (NMU)-induced breast cancer in Sprague Dawley rats. The dietary behavior of the rats was assessed, and the concentration of aluminum in the mammary glands was determined using atomic absorption spectroscopy. Genomic instability was determined in the histological sections of mammary glands stained with hematoxylin and eosin. Moreover, micronucleus in peripheral blood and comet assays were performed. The results of dietary behavior evaluation indicated no significant differences between the experimental treatments. However, aluminum concentration in breast tissues was high in the +2000Al/−NMU treatment. This experimental treatment caused moderate intraductal cell proliferation, lymph node hyperplasia, and serous gland adenoma. Furthermore, micronucleus and comet test results revealed that +2000Al/−NMU led to a genotoxic effect after a 10-day exposure and the damage was more evident after a 15-day exposure. Therefore, in conclusion, genomic instability is present and the experimental conditions assessed are not associated with breast cancer.

Dietary behavior and achievement in Sprague Dawley rats treated with aluminum and histopathologic evaluation of the murine model treated with N-nitroso-N-methylurea (NMU) as inducing agent

2016 ◽  
Vol 259 ◽  
pp. S167-S168 ◽  
Author(s):  
A.M. García-Alegría ◽  
A. Gómez-Álvarez ◽  
I. Anduro-Corona ◽  
A. Burgos-Hernández ◽  
E. Ruiz-Bustos ◽  
...  
Keyword(s):  
Murine Model ◽  
Dietary Behavior ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Histopathologic Evaluation

scholarly journals Exploration of anti-breast cancer effects of Terminalia chebula extract on DMBA-induced mammary carcinoma in Sprague Dawley rats

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Deena Priscilla Henry ◽  
Jasmine Ranjan ◽  
Rajesh Kumar Murugan ◽  
Annapoorani Sivanantham ◽  
Manikandan Alagumuthu
Keyword(s):  
Breast Cancer ◽  
Plant Extract ◽  
Mammary Carcinoma ◽  
Future Perspective ◽  
Terminalia Chebula ◽  
Sprague Dawley ◽  
Analytical Technique ◽  
Isolation And Characterization ◽  
Sprague Dawley Rats ◽  
Mcf 7

Abstract Background Plant extracts are effectively acting as the natural medicinal cocktail, non-side effective, efficacious, and freely available. The present study aimed to unveil the pharmacological and medicinal effects of Terminalia chebula plant extract in 7,12-dimethylbenzanthracene (DMBA)-induced mammary carcinoma in Sprague Dawley rats. The plant extract obtained was subjected to in vivo antioxidant and anticancer studies in various concentrations after an analytical technique such as FTIR, GCMS, and HPLC-based chemo-profiling in Sprague Dawley rats. Results Apart from the antiproliferative effect on breast cancer cell line (MCF-7) and normal breast epithelial cells (MCF-10a), we have measured the changes in body weight, along with other tumor parameters such as tumor volume, tumor incidence, tumor weight, tumor burden, serum biochemical parameters, and histopathological findings of breast tissue. As the oxidative stress further enhances the development of cancer, the antioxidant property of the plant extract demonstrates its use against cancer treatment. One hundred fifty milligrams per milliliter (IC50 250 μg/mL) concentration of the ethanolic extract was vital for the proliferation of MCF-7 cell lines (Fig. 7a). Meanwhile, 300 μg/mL (IC50 150 μg/mL) was an effective dose to attain a maximum HDAC inhibition of 78%. Also, the normal liver and kidney functioning revealed the non-toxicity nature of the plant. Conclusion Terminalia chebula could be one of the effective naturally obtained anti-breast cancer medications. Isolation and characterization of individual bioactive compounds of T. chebula would be the future perspective.

Preventive effect of berberine against DMBA-induced breast cancer in female Sprague Dawley rats

2017 ◽  
Vol 92 ◽  
pp. 207-214 ◽  
Author(s):  
Kalyani Chowdary Karnam ◽  
Maheswara Ellutla ◽  
Lakshmi Narendra Bodduluru ◽  
Eshvendar Reddy Kasala ◽  
Shravan Kumar Uppulapu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Preventive Effect ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Tissue-specific expression of the rat alpha 2u globulin gene family.

1986 ◽  
Vol 6 (10) ◽  
pp. 3563-3567 ◽  
Author(s):  
J I MacInnes ◽  
E S Nozik ◽  
D T Kurtz
Keyword(s):  
Gene Family ◽  
Developmental Regulation ◽  
Mammary Glands ◽  
Protein Isoforms ◽  
Low Molecular Weight ◽  
Sprague Dawley ◽  
Specific Expression ◽  
Gene Sets ◽  
Sprague Dawley Rats ◽  
Globulin Gene

The rat alpha 2u globulin gene family encodes approximately 20 low-molecular-weight (20,000) proteins with pIs ranging from 4.5 to 7.9. alpha 2u globulin protein isoforms were detected in the liver and in the submaxillary, lachrymal, preputial, and mammary glands of Sprague-Dawley rats. The hormonal and developmental regulation of alpha 2u globulin synthesis in each of these tissues was unique, and it appears that different alpha 2u gene sets were transcribed in the various tissues.

Antiproliferative and Apoptotic Effects of Shemamruthaa, a Herbal Preparation, in 7,12-Dimethylbenz(a)Anthracene-Induced Breast Cancer Rats

2015 ◽  
Vol 20 (4) ◽  
pp. 259-268 ◽  
Author(s):  
Ayyakkannu Purushothaman ◽  
Elumalai Nandhakumar ◽  
Palanivelu Shanthi ◽  
Thiruvaiyaru Panchanatham Sachidanandam
Keyword(s):  
Breast Cancer ◽  
Mammary Carcinoma ◽  
Nuclear Antigen ◽  
Herbal Preparation ◽  
Sprague Dawley ◽  
Protein Levels ◽  
Anticancer Effects ◽  
Sprague Dawley Rats ◽  
Apoptotic Effects ◽  
Proliferating Cell

A herbal preparation, Shemamruthaa (SM), was formulated to investigate the molecular mechanism by which it exhibits anticancer effects in mammary carcinoma bearing rats. Female Sprague-Dawley rats were used for the study, and mammary carcinoma was induced by administration of 7,12-dimethylbenz( a)anthracene, intragastrically. After 3 months of induction period, the rats were treated with SM (400 mg/kg body weight) for 14 days. Our study shows that SM-treated mammary carcinoma rats showed regression in tumor volume with concomitant increase in p53, Bax, caspase-3, and caspase-9 mRNA and protein levels compared with mammary carcinoma–induced rats. Proliferating cell nuclear antigen and anti-apoptotic Bcl-2 were markedly increased in mammary carcinoma–induced rats, whereas the SM treatment significantly decreased the expression of these proteins. The expression pattern of apoptotic signaling molecules analyzed in the present study signifies the therapeutic efficacy of SM against breast cancer.

Effects of Dehydroepiandrosterone on MNU-Induced Breast Cancer in Sprague-Dawley Rats

2006 ◽  
Vol 774 (1) ◽  
pp. 340-341 ◽  
Author(s):  
R. A. LUBET ◽  
D. M. McCORMICK ◽  
G. M. GORDON ◽  
C. GRUBBS ◽  
X-D LEI ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Sprague Dawley ◽  
Sprague Dawley Rats

scholarly journals Dietary effect of mead acid on DMBA‑induced breast cancer in female Sprague‑Dawley rats

2020 ◽  
Vol 1 (2) ◽  
pp. 1-1
Author(s):  
Yuichi Kinoshita ◽  
Masahiro Yoshioka ◽  
Yuko Emoto ◽  
Takashi  Yuri ◽  
Michiko Yuki ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Sprague Dawley ◽  
Mead Acid ◽  
Sprague Dawley Rats
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