scholarly journals Crocetin Improves Dengue Virus-Induced Liver Injury

Viruses ◽  
2020 ◽  
Vol 12 (8) ◽  
pp. 825
Author(s):  
Gopinathan Pillai Sreekanth ◽  
Aporn Chuncharunee ◽  
Pa-thai Yenchitsomanus ◽  
Thawornchai Limjindaporn
Keyword(s):  
Polymerase Chain Reaction ◽  
Liver Injury ◽  
Dengue Virus ◽  
Real Time ◽  
Nuclear Translocation ◽  
Virus Production ◽  
Denv Infection ◽  
Chain Reaction ◽  
Gene Expressions ◽  
Polymerase Chain

Dengue virus (DENV) infection is one of the most widespread mosquito-borne viral infections. Liver injury is commonly observed in severe DENV infection, and the present study aimed to examine the efficacy of crocetin treatment in an immunocompetent mouse model of DENV infection exhibiting liver injury. The efficacy of crocetin treatment in DENV-induced liver injury was assessed via both transaminase levels and histopathology analysis. A real-time polymerase chain reaction array was then used to describe the expression of 84 apoptosis-related genes. Using real-time RT-PCR and Western blot analysis, the gene expressions of host factors were investigated. Additionally, the effect of crocetin in NF-kB signaling during DENV infection was studied. We did not observe any significant reduction in virus production when DENV-infected mice were treated with crocetin. However, DENV-infected mice treated with crocetin showed reduced DENV-induced apoptosis. The real-time polymerase chain reaction array revealed pro-inflammatory cytokine expressions to be significantly reduced in the crocetin-treated DENV-infected mice. We also found that crocetin could effectively modulate antioxidant status in DENV-infected mice. Moreover, crocetin demonstrated the ability to reduce the nuclear translocation of NF-kB in DENV-infected mice. Our results suggest that crocetin treatment does not inhibit DENV replication in the liver of DENV-infected mice; however, we did find that crocetin improves host responses that reduce liver injury.

Novel pan-serotype control RNA for dengue virus typing through real-time reverse transcription-polymerase chain reaction

2019 ◽  
Vol 271 ◽  
pp. 113677
Author(s):  
Diego A. Álvarez-Díaz ◽  
Paula A. Quintero ◽  
Dioselina Peláez-Carvajal ◽  
Nadim J. Ajami ◽  
Jose A. Usme-Ciro
Keyword(s):  
Polymerase Chain Reaction ◽  
Dengue Virus ◽  
Real Time ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Virus Typing

scholarly journals Optimization of Real-time Reverse Transcriptase Polymerase Chain Reaction for Detection of Dengue Virus

2020 ◽  
pp. 1-7
Author(s):  
Kaunara A. Azizi ◽  
Arnold J. Ndaro ◽  
Athanasia Maro ◽  
Adonira Saro ◽  
Reginald A. Kavishe
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Dengue Virus ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Acid Extraction ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Reliable Technique ◽  
Polymerase Chain

Aims: This study was set to optimize conditions for real time reverse transcriptase polymerase chain reaction (RT-PCR) for detection of dengue virus by using rapid and simple nucleic acid extraction method. Methodology: One step and two step real time RT-PCR were evaluated in different PCR thermocyclers. Extraction of viral RNA was done by using a simple boom method. Results: The real time RT-PCR technique was successfully optimized using simple and rapid method for purification of nucleic acid, ‘boom method’. The technique works better when performed in a two-step procedure and can works well with all range of real time PCR machines. The optimized real time RT-PCR used in the present study is a valuable and reliable technique for routine diagnosis of dengue. Further investigation on the cost effectiveness in adopting this technique for routine screening and monitoring of the dengue infection should be done.

scholarly journals Detection of infectious dengue virus by selective real-time quantitative polymerase chain reaction

2016 ◽  
Vol 31 (4) ◽  
pp. 342-345 ◽  
Author(s):  
Xin Huang ◽  
Xuan Zhou ◽  
Xiaoyan He ◽  
Pei Wang ◽  
Shuai Yue ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dengue Virus ◽  
Real Time ◽  
Quantitative Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Evaluation of Pre-Analytical Variables in the Quantification of Dengue Virus by Real-Time Polymerase Chain Reaction

2009 ◽  
Vol 11 (6) ◽  
pp. 537-542 ◽  
Author(s):  
Azlinda Anwar ◽  
Guoqiang Wan ◽  
Kaw-Bing Chua ◽  
Joseph Thomas August ◽  
Heng-Phon Too
Keyword(s):  
Polymerase Chain Reaction ◽  
Dengue Virus ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Persistence of Dengue Virus (DENV-1, 2, 3,4) Transovarial-Transgenerational with Realtime Polymerase Chain Reaction (qPCR) in Ae. Aegypti and Ae. Albopictus (Diptera: Culicidae)

2021 ◽  
Vol 4 (8) ◽  
pp. 01-09
Author(s):  
Isna Hikmawati ◽  
Hendro Wahjono ◽  
Martini Martini ◽  
Edi Darmana ◽  
Soeharyo Hadisaputro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dengue Virus ◽  
Real Time ◽  
Virus Detection ◽  
P Value ◽  
Chain Reaction ◽  
Laboratory Colony ◽  
Research Population ◽  
Quasi Experimental ◽  
Polymerase Chain

Ae. aegypti and Ae. albopictus have an important role in DHF transmission because they can simultaneously transmit the dengue virus vertically / transovarially or horizontally. This phenomenon indicates the persistence of the dengue virus by vectors. The aim of this research was to prove the persistence of the transovarial-transgenerational dengue virus (DENV-1,2,3,4) with real time polymerase chain reaction (qPCR) in Ae. aegypti and Ae. albopictus (Diptera: Culicidae). Quasi experimental design with intervention infects DENV 1-2-3-4 serotypes in Ae. aegypti and Ae. albopictus intratoracally. Research population Ae. aegypti and Ae. albopictus laboratory colony females. Dengue virus detection uses real-time polymerase chain reaction (qPCR). Transovarial detection by qPCR indicates detection of dengue virus in Ae. albopictus DENV-1 to progeny 1 (F1), DENV-2 and DENV-3 to F2, DENV-4 to F3. Next to Ae. aegypti DENV-1 to 1st progeny (F1), DENV-2 to F2, DENV-3 to F4 and DENV-4 to F3. there was no difference in MIR value (p value: 0.356) for the four serotypes in Ae. albopictus and Ae. aegypti. DENV-3 is the most persistent serotype in Ae. aegypti with 83.3% MIR and DENV-4 were the most persistent serotypes in Ae.albopictus with 100% MIR. The need to improve vector control models that focus not only on the main vector, but also other co-vectors.

scholarly journals Optimization Of Real-Time Reverse Transcriptase Polymerase Chain Reaction For Detection Of Dengue Virus

2020 ◽  
Author(s):  
Kaunara Ally Azizi ◽  
Arnold J Ndaro ◽  
Athanasia Maro ◽  
Adonira T Saro ◽  
Reginald Kavishe
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Dengue Virus ◽  
Reverse Transcriptase ◽  
Real Time ◽  
Diagnostic Methods ◽  
Virus Infections ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Objective Rapid and accurate laboratory confirmatory is very essential for control measures of dengue virus infections. However, many cases of dengue virus infections in most of the hospitals remain undiagnosed due to presence of other febrile illnesses with overlapping symptoms and lack of specificity in most of laboratory diagnostic methods. This study was set to optimize conditions for real time reverse transcriptase polymerase chain reaction (RT-PCR) for detection of dengue virus by using rapid and simple nucleic acid extraction method.Results The real time RT-PCR technique was successfully optimized using simple and rapid method for purification of nucleic acid, ‘boom method’. The technique works better when performed in a two-step procedure and can works well with all range of real time PCR machines. The optimized real time RT-PCR used in the present study is a valuable and reliable technique for routine diagnosis of dengue. Further investigation on the cost effectiveness in adopting this technique for routine screening and monitoring of the dengue infection should be done.

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