scholarly journals Atovaquone and Berberine Chloride Reduce SARS-CoV-2 Replication In Vitro

Viruses ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2437
Author(s):  
Bruno A. Rodriguez-Rodriguez ◽  
Maria G. Noval ◽  
Maria E. Kaczmarek ◽  
Kyung Ku Jang ◽  
Sara A. Thannickal ◽  
...  
Keyword(s):  
Human Lung ◽  
Direct Interaction ◽  
Holistic Medicine ◽  
Lung Cells ◽  
Emerging Viruses ◽  
Berberine Chloride ◽  
Human Lung Cells ◽  
Emerging Virus ◽  
To Come

Epidemic RNA viruses seem to arise year after year leading to countless infections and devastating disease. SARS-CoV-2 is the most recent of these viruses, but there will undoubtedly be more to come. While effective SARS-CoV-2 vaccines are being deployed, one approach that is still missing is effective antivirals that can be used at the onset of infections and therefore prevent pandemics. Here, we screened FDA-approved compounds against SARS-CoV-2. We found that atovaquone, a pyrimidine biosynthesis inhibitor, is able to reduce SARS-CoV-2 infection in human lung cells. In addition, we found that berberine chloride, a plant-based compound used in holistic medicine, was able to inhibit SARS-CoV-2 infection in cells through direct interaction with the virion. Taken together, these studies highlight potential avenues of antiviral development to block emerging viruses. Such proactive approaches, conducted well before the next pandemic, will be essential to have drugs ready for when the next emerging virus hits.

scholarly journals Polyphosphate Reverses the Toxicity of the Quasi-Enzyme Bleomycin on Alveolar Endothelial Lung Cells In Vitro

Cancers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 750
Author(s):  
Werner E. G. Müller ◽  
Meik Neufurth ◽  
Shunfeng Wang ◽  
Heinz C. Schröder ◽  
Xiaohong Wang
Keyword(s):  
Dna Damage ◽  
Human Lung ◽  
Dna Integrity ◽  
Lung Cells ◽  
Antitumor Antibiotic ◽  
Cell Toxicity ◽  
Inorganic Polyphosphate ◽  
Human Lung Cells ◽  
Anti Cancer

The anti-cancer antitumor antibiotic bleomycin(s) (BLM) induces athyminic sites in DNA after its activation, a process that results in strand splitting. Here, using A549 human lung cells or BEAS-2B cells lunc cells, we show that the cell toxicity of BLM can be suppressed by addition of inorganic polyphosphate (polyP), a physiological polymer that accumulates and is released from platelets. BLM at a concentration of 20 µg ml−1 causes a decrease in cell viability (by ~70%), accompanied by an increased DNA damage and chromatin expansion (by amazingly 6-fold). Importantly, the BLM-caused effects on cell growth and DNA integrity are substantially suppressed by polyP. In parallel, the enlargement of the nuclei/chromatin in BLM-treated cells (diameter, 20–25 µm) is normalized to ~12 µm after co-incubation of the cells with BLM and polyP. A sequential application of the drugs (BLM for 3 days, followed by an exposure to polyP) does not cause this normalization. During co-incubation of BLM with polyP the gene for the BLM hydrolase is upregulated. It is concluded that by upregulating this enzyme polyP prevents the toxic side effects of BLM. These data might also contribute to an application of BLM in COVID-19 patients, since polyP inhibits binding of SARS-CoV-2 to cellular ACE2.

Pathobiological Effects of Fibers and Tobacco-Related Chemicals in Human Lung Cells In Vitro

1989 ◽  
pp. 103-117
Author(s):  
C. C. Harris ◽  
J. C. Willey ◽  
N. Matsukura ◽  
J. F. Lechner ◽  
M. Miyashita ◽  
...  
Keyword(s):  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells

Genomic responses of human lung cells exposure through a successful in vitro field deployment in Houston, Texas

2017 ◽  
Vol 280 ◽  
pp. S212
Author(s):  
Hang Nguyen ◽  
Kenneth Sexton ◽  
Lisa Smeester ◽  
Kjersti Marie Aagaard ◽  
Cynthia Do Shope ◽  
...  
Keyword(s):  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Field Deployment

Quantitation and characterization of leukotriene products released following immunologic stimulation of human lung cells in vitro

CHEST Journal ◽  
1983 ◽  
Vol 83 (5) ◽  
pp. 81S-82 ◽  
Author(s):  
A. G. Leitch ◽  
R. A. Lewis ◽  
E. J. Corey ◽  
K. F. Austen
Keyword(s):  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Stimulation Of

scholarly journals Influence of the physicochemical features of TiO2 nanoparticles on the formation of a protein corona and impact on cytotoxicity

RSC Advances ◽  
2020 ◽  
Vol 10 (72) ◽  
pp. 43950-43959
Author(s):  
Ozge Kose ◽  
Marion Stalet ◽  
Lara Leclerc ◽  
Valérie Forest
Keyword(s):  
Tio2 Nanoparticles ◽  
Human Lung ◽  
Protein Corona ◽  
Lung Cells ◽  
Human Lung Cells ◽  
Potential Relationship ◽  
Physicochemical Features

Characterization of the formation of the protein corona of TiO2 nanoparticles as a function of the main nanoparticle properties and investigation of potential relationship with the cytotoxicity nanoparticles induce in vitro in human lung cells.

Biokinetics of Aerosolized Liposomal Ciclosporin A in Human Lung Cells In Vitro Using an Air-Liquid Cell Interface Exposure System

2017 ◽  
Vol 30 (6) ◽  
pp. 411-424 ◽  
Author(s):  
Otmar Schmid ◽  
Corinne Jud ◽  
Yuki Umehara ◽  
Dominik Mueller ◽  
Albert Bucholski ◽  
...  
Keyword(s):  
Human Lung ◽  
Lung Cells ◽  
Exposure System ◽  
Human Lung Cells ◽  
Ciclosporin A ◽  
Liquid Cell ◽  
Cell Interface

scholarly journals Comparison of Micro- and Nanoscale Fe+3–Containing (Hematite) Particles for Their Toxicological Properties in Human Lung Cells In Vitro

2012 ◽  
Vol 126 (1) ◽  
pp. 173-182 ◽  
Author(s):  
Kunal Bhattacharya ◽  
Eik Hoffmann ◽  
Roel F. P. Schins ◽  
Jens Boertz ◽  
Eva-Maria Prantl ◽  
...  
Keyword(s):  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells
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