scholarly journals Development of a Viral-Like Particle Candidate Vaccine Against Novel Variant Infectious Bursal Disease Virus

Vaccines ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 142
Author(s):  
Yulong Wang ◽  
Nan Jiang ◽  
Linjin Fan ◽  
Li Gao ◽  
Kai Li ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Protective Efficacy ◽  
Expression System ◽  
Size Exclusion ◽  
Candidate Vaccine ◽  
Infectious Bursal Disease ◽  
Immune Protection ◽  
Bursal Disease ◽  
Novel Variant

Infectious bursal disease (IBD), an immunosuppressive disease of young chickens, is caused by infectious bursal disease virus (IBDV). Novel variant IBDV (nVarIBDV), a virus that can evade immune protection against very virulent IBDV (vvIBDV), is becoming a threat to the poultry industry. Therefore, nVarIBDV-specific vaccine is much needed for nVarIBDV control. In this study, the VP2 protein of SHG19 (a representative strain of nVarIBDV) was successfully expressed using an Escherichia coli expression system and further purified via ammonium sulfate precipitation and size-exclusion chromatography. The purified protein SHG19-VP2-466 could self-assemble into 25-nm virus-like particle (VLP). Subsequently, the immunogenicity and protective effect of the SHG19-VLP vaccine were evaluated using animal experiments, which indicated that the SHG19-VLP vaccine elicited neutralization antibodies and provided 100% protection against the nVarIBDV. Furthermore, the protective efficacy of the SHG19-VLP vaccine against the vvIBDV was evaluated. Although the SHG19-VLP vaccine induced a comparatively lower vvIBDV-specific neutralization antibody titer, it provided good protection against the lethal vvIBDV. In summary, the SHG19-VLP candidate vaccine could provide complete immune protection against the homologous nVarIBDV as well as the heterologous vvIBDV. This study is of significance to the comprehensive prevention and control of the recent atypical IBD epidemic.

Novel variant strains of infectious bursal disease virus isolated in China

2019 ◽  
Vol 230 ◽  
pp. 212-220 ◽  
Author(s):  
Linjin Fan ◽  
Tiantian Wu ◽  
Altaf Hussain ◽  
Yulong Gao ◽  
Xianying Zeng ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Novel Variant

A reassortment vaccine candidate of the novel variant infectious bursal disease virus

2020 ◽  
Vol 251 ◽  
pp. 108905
Author(s):  
Linjin Fan ◽  
Yulong Wang ◽  
Nan Jiang ◽  
Li Gao ◽  
Kai Li ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Vaccine Candidate ◽  
Infectious Bursal Disease ◽  
The Novel ◽  
Bursal Disease ◽  
Novel Variant

scholarly journals Novel variant infectious bursal disease virus suppresses Newcastle disease vaccination in broiler and layer chickens

2020 ◽  
Vol 99 (12) ◽  
pp. 6542-6548
Author(s):  
Linjin Fan ◽  
Yulong Wang ◽  
Nan Jiang ◽  
Mango Chen ◽  
Li Gao ◽  
...  
Keyword(s):  
Disease Virus ◽  
Newcastle Disease ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Novel Variant ◽  
Layer Chickens

scholarly journals Genotyping and Molecular Characterization of Infectious Bursal Disease Virus Identified in Important Poultry-Raising Areas of China During 2019 and 2020

2021 ◽  
Vol 8 ◽  
Author(s):  
Nan Jiang ◽  
Yulong Wang ◽  
Wenying Zhang ◽  
Xinxin Niu ◽  
Mengmeng Huang ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Hypervariable Region ◽  
Infectious Bursal Disease ◽  
The Novel ◽  
Poultry Industry ◽  
Vp2 Gene ◽  
Healthy Development ◽  
Bursal Disease ◽  
Novel Variant

Infectious bursal disease (IBD) is an acute and highly contagious immunosuppressive disease caused by the infectious bursal disease virus (IBDV), which seriously threatens the healthy development of the poultry industry. Since its spread to China in the early 1990s, the very virulent IBDV (vvIBDV) characterized by high lethality, has been the focus of prevention and control. However, the novel variant IBDV (nVarIBDV), which has been widely prevalent in China since 2017, has brought a new threat to the poultry industry. In this study, the prevalence of IBDV in the important poultry-raising areas of China from 2019 to 2020 was detected. Of these, 45.1% (101/224) of the samples and 61.9% (26/42) of the chicken flocks were shown to be positive for IBDV. For 50 IBDVs, the sequences of the hypervariable region of the VP2 gene in segment A and of the B-marker of the VP1 gene in segment B were analyzed. The results revealed the coexistence of a number of different IBDV genotypes, including A2dB1 (nVar, 26/50, 52.0%), A3B3 (HLJ0504-like, 15/50, 30.0%), A1B1 (classical, 1/50, 2.0%), and A8B1 (attenuated, 1/50, 2.0%). This indicated that the newly emerging nVarIBDV of A2dB1 and the persistently circulating HLJ0504-like vvIBDV of A3B3 are the two important epidemic strains. Furthermore, we established that segment reassortment has occurred among these circulating strains. This study is the first to reveal the novel epidemic characteristics of IBDV since the report of the emerging nVarIBDV of A2dB1 in China.

scholarly journals Recombinant Lactococcus Expressing a Novel Variant of Infectious Bursal Disease Virus VP2 Protein Can Induce Unique Specific Neutralizing Antibodies in Chickens and Provide Complete Protection

Viruses ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1350
Author(s):  
Zhihao Wang ◽  
Jielan Mi ◽  
Yulong Wang ◽  
Tingting Wang ◽  
Xiaole Qi ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Neutralizing Antibodies ◽  
Infectious Bursal Disease ◽  
Promising Candidate ◽  
Conventional Elisa ◽  
Bursal Disease ◽  
Protection Rate ◽  
Novel Variant ◽  
Recombinant Lactococcus Lactis

Recent reports of infectious bursal disease virus (IBDV) infections in China, Japan, and North America have indicated the presence of variant, and the current conventional IBDV vaccine cannot completely protect against variant IBDV. In this study, we constructed recombinant Lactococcus lactis (r-L. lactis) expressing a novel variant of IBDV VP2 (avVP2) protein along with the Salmonella resistance to complement killing (RCK) protein, and Western blotting analysis confirmed that r-L. lactis successfully expressed avVP2-RCK fusion protein. We immunized chickens with this vaccine and subsequently challenged them with the very virulent IBDV (vvIBDV) and a novel variant wild IBDV (avIBDV) to evaluate the immune effect of the vaccine. The results show that the r-L. lactis-avVP2-RCK-immunized group exhibited a 100% protection rate when challenged with avIBDV and 100% survival rate to vvIBDV. Furthermore, this immunization resulted in the production of unique neutralizing antibodies that cannot be detected by conventional ELISA. These results indicate that r-L. lactis-avVP2-RCK is a promising candidate vaccine against IBDV infections, which can produce unique neutralizing antibodies that cannot be produced by other vaccines and protect against IBDV infection, especially against the variant strain.

scholarly journals The Maturation Process of pVP2 Requires Assembly of Infectious Bursal Disease Virus Capsids

2002 ◽  
Vol 76 (5) ◽  
pp. 2384-2392 ◽  
Author(s):  
Christophe Chevalier ◽  
Jean Lepault ◽  
Inge Erk ◽  
Bruno Da Costa ◽  
Bernard Delmas
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Fluorescent Protein ◽  
Expression System ◽  
Baculovirus Expression System ◽  
Infectious Bursal Disease ◽  
Maturation Process ◽  
Final Maturation ◽  
Bursal Disease ◽  
Green Fluorescent

ABSTRACT Infectious bursal disease virus (IBDV) is a nonenveloped avian virus with a two-segment double-stranded RNA genome. Its T=13 icosahedral capsid is most probably assembled with 780 subunits of VP2 and 600 copies of VP3 and has a diameter of about 60 nm. VP1, the RNA-dependent RNA polymerase, resides inside the viral particle. Using a baculovirus expression system, we first observed that expression of the pVP2-VP4-VP3 polyprotein encoded by the genomic segment IBDA results mainly in the formation of tubules with a diameter of about 50 nm and composed of pVP2, the precursor of VP2. Very few virus-like particles (VLPs) and VP4 tubules with a diameter of about 25 nm were also identified. The inefficiency of VLP assembly was further investigated by expression of additional IBDA-derived constructs. Expression of pVP2 without any other polyprotein components results in the formation of isometric particles with a diameter of about 30 nm. VLPs were observed mainly when a large exogeneous polypeptide sequence (the green fluorescent protein sequence) was fused to the VP3 C-terminal domain. Large numbers of VLPs were visualized by electron microscopy, and single particles were shown to be fluorescent by standard and confocal microscopy analysis. Moreover, the final maturation process converting pVP2 into the VP2 mature form was observed on generated VLPs. We therefore conclude that the correct scaffolding of the VP3 can be artificially induced to promote the formation of VLPs and that the final processing of pVP2 to VP2 is controlled by this particular assembly. To our knowledge, this is the first report of the engineering of a morphogenesis switch to control a particular type of capsid protein assembly.

Development of a recombinant VP2 vaccine for the prevention of novel variant strains of infectious bursal disease virus

2020 ◽  
Vol 49 (6) ◽  
pp. 557-571 ◽  
Author(s):  
Guopan Li ◽  
Hongyan Kuang ◽  
Huaxiong Guo ◽  
Lianshen Cai ◽  
Dianfeng Chu ◽  
...  
Keyword(s):  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease ◽  
Novel Variant
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