Efficiency assessment of ion-exchange chromatography as the final stage of anti-VEGF antibody purification

2021 ◽  
pp. 6-9
Author(s):  
Denis Alekseevich Mazalyov ◽  
Aleksey Vladimirovich Tsivov ◽  
Viktor Andreevich Kuvshinov ◽  
Mariya Nikolaevna Zavorueva ◽  
Aleksandr Alekseevich Smirnov ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Final Stage ◽  
Therapeutic Agent ◽  
Medical Application ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Chromatographic Purification ◽  
Antibody Purification ◽  
Efficiency Assessment ◽  
Anti Vegf

The article presents the results of chromatographic purification of the anti-VEGF antibody using the ion-exchange sorbent CaptoТМ S and considers the prospects for medical application of the antibody as a therapeutic agent for cancer treatment.

Membrane ion-exchange chromatography for process-scale antibody purification

2001 ◽  
Vol 907 (1-2) ◽  
pp. 145-154 ◽  
Author(s):  
Heather L Knudsen ◽  
Robert L Fahrner ◽  
Yuan Xu ◽  
Lenore A Norling ◽  
Gregory S Blank
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Antibody Purification ◽  
Process Scale

Scaleup of monoclonal antibody purification using radial streaming ion exchange chromatography

1988 ◽  
Vol 32 (3) ◽  
pp. 326-333 ◽  
Author(s):  
A. Jungbauer ◽  
F. Unterluggauer ◽  
K. Uhl ◽  
A. Buchacher ◽  
F. Steindl ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Antibody Purification

PURIFICATION, CHARACTERIZATION, AND EVALUATION OF FIBRINOLYTIC ACTIVITY OF STAPHYLOKINASE FROM LOCALLY ISOLATED STAPHYLOCOCCUS AUREUS GH38

2020 ◽  
Vol 51 (4) ◽  
pp. 1195-1203
Author(s):  
Noori & Aziz
Keyword(s):  
Staphylococcus Aureus ◽  
Sodium Chloride ◽  
Ion Exchange ◽  
Fibrinolytic Activity ◽  
Therapeutic Agent ◽  
Blood Clot ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Iron Chloride ◽  
Ammonium Sulfate Precipitation

This study was aimed to purify, characteristic, and fibrinolytic activity of staphylokinase (SAK), is an enzyme activates plasminogen to form plasmin, which digest fibrin clots that cause thrombosis clot. Staphylokinase was purified from local isolate Staphylococcus aureus GH38 by ammonium sulfate precipitation at 70% saturation followed by ion exchange chromatography (CM-Cellulose) with a purification fold 2.73, and 1.53, and recovery 72.1, and 33.11% in wash and elution steps respectively. The partially purified enzyme was high activity at 40°C with pH 7 and the enzyme retained 100% of its activity at 35°C with pH 7. The activity of an enzyme increased by its treatment with calcium and sodium chloride, while the activity affected when incubated with mercury, silver, and iron chloride. The enzyme have high effective against thrombus (blood clot), which encourage the use of enzyme in the treatment as therapeutic agent to remove clots formed in the human body.

scholarly journals Multi-element ion-exchange chromatography and high-precision MC-ICP-MS isotope analysis of Mg and Ti from sub-mm-sized meteorite inclusions

2018 ◽  
Vol 33 (4) ◽  
pp. 613-628 ◽  
Author(s):  
K. K. Larsen ◽  
D. Wielandt ◽  
M. Bizzarro
Keyword(s):  
Ion Exchange ◽  
High Precision ◽  
Isotope Analysis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Chromatographic Purification ◽  
Icp Ms ◽  
Purification Protocol

We present a refined multi-element (Mg, Ca, Ti, V, Cr, Fe, Ni, Zr, Mo, Ru, Hf and W) ion chromatographic purification protocol applied to high-precision isotope analysis of Mg and Ti using MC-ICP-MS.

Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification

2015 ◽  
Vol 16 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Tobias Hahn ◽  
Thiemo Huuk ◽  
Anna Osberghaus ◽  
Katharina Doninger ◽  
Susanne Nath ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Inverse Modeling ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Antibody Purification ◽  
Calibration Free

Studies on an Inhibitor of Plasminogen Activation in Human Serum

1973 ◽  
Vol 30 (02) ◽  
pp. 414-424 ◽  
Author(s):  
Ulla Hedner
Keyword(s):  
Ion Exchange ◽  
Human Serum ◽  
Antithrombin Iii ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Specific Adsorption ◽  
Partial Purification ◽  
Plasminogen Activation ◽  
Preparative Electrophoresis

SummaryA procedure is described for partial purification of an inhibitor of the activation of plasminogen by urokinase and streptokinase. The method involves specific adsorption of contammants, ion-exchange chromatography on DEAE-Sephadex, gel filtration on Sephadex G-200 and preparative electrophoresis. The inhibitor fraction contained no antiplasmin, no plasminogen, no α1-antitrypsin, no antithrombin-III and was shown not to be α2 M or inter-α-inhibitor. It contained traces of prothrombin and cerulo-plasmin. An antiserum against the inhibitor fraction capable of neutralising the inhibitor in serum was raised in rabbits.

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