Antibody Purification: Ion-Exchange Chromatography

2009 ◽  
pp. 27-32 ◽  
Author(s):  
Ana Cristina Grodzki ◽  
Elsa Berenstein
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Antibody Purification

Membrane ion-exchange chromatography for process-scale antibody purification

2001 ◽  
Vol 907 (1-2) ◽  
pp. 145-154 ◽  
Author(s):  
Heather L Knudsen ◽  
Robert L Fahrner ◽  
Yuan Xu ◽  
Lenore A Norling ◽  
Gregory S Blank
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Antibody Purification ◽  
Process Scale

Scaleup of monoclonal antibody purification using radial streaming ion exchange chromatography

1988 ◽  
Vol 32 (3) ◽  
pp. 326-333 ◽  
Author(s):  
A. Jungbauer ◽  
F. Unterluggauer ◽  
K. Uhl ◽  
A. Buchacher ◽  
F. Steindl ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Antibody Purification

Efficiency assessment of ion-exchange chromatography as the final stage of anti-VEGF antibody purification

2021 ◽  
pp. 6-9
Author(s):  
Denis Alekseevich Mazalyov ◽  
Aleksey Vladimirovich Tsivov ◽  
Viktor Andreevich Kuvshinov ◽  
Mariya Nikolaevna Zavorueva ◽  
Aleksandr Alekseevich Smirnov ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Final Stage ◽  
Therapeutic Agent ◽  
Medical Application ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Chromatographic Purification ◽  
Antibody Purification ◽  
Efficiency Assessment ◽  
Anti Vegf

The article presents the results of chromatographic purification of the anti-VEGF antibody using the ion-exchange sorbent CaptoТМ S and considers the prospects for medical application of the antibody as a therapeutic agent for cancer treatment.

Calibration-free inverse modeling of ion-exchange chromatography in industrial antibody purification

2015 ◽  
Vol 16 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Tobias Hahn ◽  
Thiemo Huuk ◽  
Anna Osberghaus ◽  
Katharina Doninger ◽  
Susanne Nath ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Inverse Modeling ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Antibody Purification ◽  
Calibration Free

Studies on an Inhibitor of Plasminogen Activation in Human Serum

1973 ◽  
Vol 30 (02) ◽  
pp. 414-424 ◽  
Author(s):  
Ulla Hedner
Keyword(s):  
Ion Exchange ◽  
Human Serum ◽  
Antithrombin Iii ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Specific Adsorption ◽  
Partial Purification ◽  
Plasminogen Activation ◽  
Preparative Electrophoresis

SummaryA procedure is described for partial purification of an inhibitor of the activation of plasminogen by urokinase and streptokinase. The method involves specific adsorption of contammants, ion-exchange chromatography on DEAE-Sephadex, gel filtration on Sephadex G-200 and preparative electrophoresis. The inhibitor fraction contained no antiplasmin, no plasminogen, no α1-antitrypsin, no antithrombin-III and was shown not to be α2 M or inter-α-inhibitor. It contained traces of prothrombin and cerulo-plasmin. An antiserum against the inhibitor fraction capable of neutralising the inhibitor in serum was raised in rabbits.

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