Faculty Opinions recommendation of Three-dimensional analysis of syncytial-type cell plates during endosperm cellularization visualized by high resolution electron tomography.

With current advances in electron microscope design, high resolution electron microscopy has become routine, and point resolutions of better than 2Å have been obtained in images of many inorganic crystals. Although this resolution is sufficient to resolve interatomic spacings, interpretation generally requires comparison of experimental images with calculations. Since the images are two-dimensional representations of projections of the full three-dimensional structure, information is invariably lost in the overlapping images of atoms at various heights. The technique of electron crystallography, in which information from several views of a crystal is combined, has been developed to obtain three-dimensional information on proteins. The resolution in images of proteins is severely limited by effects of radiation damage. In principle, atomic-resolution, 3D reconstructions should be obtainable from specimens that are resistant to damage. The most serious problem would appear to be in obtaining high-resolution images from areas that are thin enough that dynamical scattering effects can be ignored.

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Three-dimensional analysis of plant structure using high-resolution X-ray computed tomography

Trends in Plant Science ◽

10.1016/s1360-1385(02)00004-3 ◽

2003 ◽

Vol 8 (1) ◽

pp. 2-6 ◽

Cited By ~ 100

Author(s):

Wolfgang H Stuppy ◽

Jessica A Maisano ◽

Matthew W Colbert ◽

Paula J Rudall ◽

Timothy B Rowe

Keyword(s):

Computed Tomography ◽

High Resolution ◽

Dimensional Analysis ◽

Three Dimensional ◽

Plant Structure ◽

X Ray ◽

X Ray Computed

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High-resolution three-dimensional probes of biomaterials and their interfaces

Philosophical Transactions of The Royal Society A Mathematical Physical and Engineering Sciences ◽

10.1098/rsta.2011.0253 ◽

2012 ◽

Vol 370 (1963) ◽

pp. 1337-1351 ◽

Cited By ~ 19

Author(s):

Kathryn Grandfield ◽

Anders Palmquist ◽

Håkan Engqvist

Keyword(s):

High Resolution ◽

Electron Tomography ◽

Three Dimensional ◽

Controlled Drug Release ◽

Biological Tissues ◽

Dimensional Structure ◽

Bone Interface ◽

Biomaterial Interfaces ◽

Titania Coating

Interfacial relationships between biomaterials and tissues strongly influence the success of implant materials and their long-term functionality. Owing to the inhomogeneity of biological tissues at an interface, in particular bone tissue, two-dimensional images often lack detail on the interfacial morphological complexity. Furthermore, the increasing use of nanotechnology in the design and production of biomaterials demands characterization techniques on a similar length scale. Electron tomography (ET) can meet these challenges by enabling high-resolution three-dimensional imaging of biomaterial interfaces. In this article, we review the fundamentals of ET and highlight its recent applications in probing the three-dimensional structure of bioceramics and their interfaces, with particular focus on the hydroxyapatite–bone interface, titanium dioxide–bone interface and a mesoporous titania coating for controlled drug release.

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Feasible atomic-resolution electron tomography for general crystal surfaces by quantitative reconstruction from a high-resolution image

Ultramicroscopy ◽

10.1016/j.ultramic.2019.06.002 ◽

2019 ◽

Vol 205 ◽

pp. 27-38

Author(s):

R.H. Shen ◽

W.Q. Ming ◽

J.H. Chen ◽

Y.T. He ◽

S.B. Mi ◽

...

Keyword(s):

High Resolution ◽

Electron Tomography ◽

Atomic Resolution ◽

Crystal Surfaces ◽

Quantitative Reconstruction ◽

Resolution Image ◽

High Resolution Image ◽

Resolution Electron

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A Novel Method for Automated Acquisition of Tilt Series for Electron Tomography Based on Pre-Calibration of the Specimen Stage

Microscopy and Microanalysis ◽

10.1017/s143192760003823x ◽

2000 ◽

Vol 6 (S2) ◽

pp. 1148-1149

Author(s):

U. Ziese ◽

A.H. Janssen ◽

T.P. van der Krift ◽

A.G. van Balen ◽

W.J. de Ruijter ◽

...

Keyword(s):

High Resolution ◽

3D Reconstruction ◽

Cell Biology ◽

Electron Tomography ◽

Three Dimensional ◽

Imaging Method ◽

3D Images ◽

Tilt Series ◽

3D Information ◽

Structural Arrangements

Electron tomography is a three-dimensional (3D) imaging method with transmission electron microscopy (TEM) that provides high-resolution 3D images of structural arrangements. Conventional TEM images are in first approximation mere 2D-projections of a 3D sample under investigation. With electron tomographya series of images is acquired of a sample that is tilted over a large angular range (±70°) with small angular tilt increments (so called tilt-series). For the subsequent 3D-reconstruction, the images of the tilt series are aligned relative to each other and the 3D-reconstruction is computed. Electron tomography is the only technique that can provide true 3D information with nm-scale resolution of individual and unique samples. For (cell) biology and material science applications the availability of high-resolution 3D images of structural arrangements within individual samples provides unique architectural information that cannot be obtained otherwise. Routine application of electron tomography will comprise a major revolutionary step forward in the characterization of complex materials and cellular arrangements.

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