Faculty Opinions recommendation of Cu(I)-dependent biogenesis of the galactose oxidase redox cofactor.

2003 ◽  
Author(s):  
Antonio Rosato
Keyword(s):  
Galactose Oxidase

Functional Fractionation of Platelets: Aggregation Kinetics and Glycoprotein Labeling of Differing Platelet Populations

1982 ◽  
Vol 48 (02) ◽  
pp. 211-216 ◽  
Author(s):  
V M Haver ◽  
A R L Gear
Keyword(s):  
Galactose Oxidase ◽  
Aggregation Kinetics ◽  
Maximal Rate ◽  
Membrane Glycoproteins ◽  
Whole Cells ◽  
Reversible Aggregation ◽  
Significant Difference ◽  
Small Doses ◽  
Major Loss ◽  
Kinetics Of

SummaryPlatelet heterogeneity has been studied with a technique called functional fractionation which employs gentle centrifugation to yield subpopulations (“reactive” and “less-reactive” platelets) after exposure to small doses of aggregating agent. Aggregation kinetics of the different platelet populations were investigated by quenched-flow aggregometry. The large, “reactive” platelets were more sensitive to ADP (Ka = 1.74 μM) than the smaller “less-reactive” platelets (Ka = 4.08 μM). However, their maximal rate of aggregation (Vmax, % of platelets aggregating per sec) of 23.3 was significantly lower than the “less-reactive” platelets (Vmax = 34.7). The “reactive” platelets had a 2.2 fold higher level of cyclic AMP.Platelet glycoproteins were labeled using the neuraminidase-galactose oxidase – [H3]-NaBH4 technique. When platelets were labeled after reversible aggregation, the “reactive” platelets showed a two-fold decrease in labeling efficiency (versus control platelets). However, examination of whole cells or membrane preparations from reversibly aggregated platelets revealed no significant difference in Coomassie or PAS (Schiff) staining.These results suggest that the large, “reactive” platelets are more sensitive to ADP but are not hyperaggregable in a kinetic sense. Reversible aggregation may cause a re-orientation of membrane glycoproteins that is apparently not characterized by a major loss of glycoprotein material.

scholarly journals Structure and mechanism of galactose oxidase. The free radical site.

1994 ◽  
Vol 269 (40) ◽  
pp. 25095-25105
Author(s):  
A.J. Baron ◽  
C. Stevens ◽  
C. Wilmot ◽  
K.D. Seneviratne ◽  
V. Blakeley ◽  
...  
Keyword(s):  
Free Radical ◽  
Galactose Oxidase ◽  
Radical Site

The metabolism of carbohydrates by extremely halophilic bacteria: the identification of lactobionic acid as a product of lactose metabolism by Halobacterium saccharovorum

1978 ◽  
Vol 24 (8) ◽  
pp. 898-903 ◽  
Author(s):  
Geraldine A. Tomlinson ◽  
Maureen P. Strohm ◽  
Lawrence I. Hochstein
Keyword(s):  
Thin Layer ◽  
Acid Hydrolysis ◽  
Column Chromatography ◽  
Gluconic Acid ◽  
Halophilic Bacteria ◽  
Galactose Oxidase ◽  
Lactobionic Acid ◽  
Lactose Metabolism ◽  
Furanose Form

Nongrowing cells of Halobacterium saccharovorum oxidized lactose to a product identified as lactobionic acid by thin-layer, paper, and column chromatography, and by identification of the galactose and gluconic acid produced from it after acid hydrolysis. Growing cells oxidized lactose to a product that was identical with lactobionate except that it did not serve as a substrate for galactose oxidase. While the identity of this compound has not been established, it is suggested that the product is lactobionic acid in which the galactose moeity is in the furanose form. Neither lactobionate nor the product produced by growing cells was further metabolized, suggesting that lactose oxidation is not coupled to growth.

Spectroscopic and magnetochemical studies on the active site copper complex in galactose oxidase

2000 ◽  
Vol 8 (1-3) ◽  
pp. 3-15 ◽  
Author(s):  
Mei M. Whittaker ◽  
Christopher A. Ekberg ◽  
James Peterson ◽  
Mariana S. Sendova ◽  
Edmund P. Day ◽  
...  
Keyword(s):  
Copper Complex ◽  
Active Site ◽  
Galactose Oxidase

scholarly journals Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution

2004 ◽  
Vol 17 (2) ◽  
pp. 141-148 ◽  
Author(s):  
D. Wilkinson ◽  
N. Akumanyi ◽  
R. Hurtado-Guerrero ◽  
H. Dawkes ◽  
P.F. Knowles ◽  
...  
Keyword(s):  
Directed Evolution ◽  
Kinetic Studies ◽  
Galactose Oxidase

The preparation of d-galactose-6-t and some of its derivatives by using galactose oxidase

1967 ◽  
Vol 4 (3) ◽  
pp. 267-269 ◽  
Author(s):  
J.E.G. Barnett
Keyword(s):  
Galactose Oxidase

Autoredox Interconversion of Two Galactose Oxidase Forms GOaseoxand GOasesemiwith and without Dioxygen

2001 ◽  
Vol 40 (11) ◽  
pp. 2528-2533 ◽  
Author(s):  
Craig Wright ◽  
A. Geoffrey Sykes
Keyword(s):  
Galactose Oxidase

Immunochemistry of type XIV pneumococcus capsular polysaccharide oxidized by d-galactose oxidase

1972 ◽  
Vol 9 (11) ◽  
pp. 1095-1101 ◽  
Author(s):  
Sergio Estrada-Parra ◽  
Irma Gómez
Keyword(s):  
Capsular Polysaccharide ◽  
Galactose Oxidase
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