Faculty Opinions recommendation of The knotted1-like homeobox gene BREVIPEDICELLUS regulates cell differentiation by modulating metabolic pathways.

2003 ◽  
Author(s):  
Jan Traas
Keyword(s):  
Cell Differentiation ◽  
Metabolic Pathways ◽  
Homeobox Gene

scholarly journals Key Role of the Cdx2 Homeobox Gene in Extracellular Matrix–mediated Intestinal Cell Differentiation

1997 ◽  
Vol 139 (6) ◽  
pp. 1553-1565 ◽  
Author(s):  
Olivier Lorentz ◽  
Isabelle Duluc ◽  
Adèle De Arcangelis ◽  
Patricia Simon-Assmann ◽  
Michèle Kedinger ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Differentiation ◽  
Antisense Rna ◽  
Homeobox Gene ◽  
Intestinal Cell ◽  
Cdx2 Expression ◽  
Laminin 1 ◽  
Crypt Cells

To explore the role of homeobox genes in the intestine, the human colon adenocarcinoma cell line Caco2-TC7 has been stably transfected with plasmids synthesizing Cdx1 and Cdx2 sense and antisense RNAs. Cdx1 overexpression or inhibition by antisense RNA does not markedly modify the cell differentiation markers analyzed in this study. In contrast, Cdx2 overexpression stimulates two typical markers of enterocytic differentiation: sucrase-isomaltase and lactase. Cells in which the endogenous expression of Cdx2 is reduced by antisense RNA attach poorly to the substratum. Conversely, Cdx2 overexpression modifies the expression of molecules involved in cell–cell and cell–substratum interactions and in transduction process: indeed, E-cadherin, integrin-β4 subunit, laminin-γ2 chain, hemidesmosomal protein, APC, and α-actinin are upregulated. Interestingly, most of these molecules are preferentially expressed in vivo in the differentiated villi enterocytes rather than in crypt cells. Cdx2 overexpression also results in the stimulation of HoxA-9 mRNA expression, an homeobox gene selectively expressed in the colon. In contrast, Cdx2-overexpressing cells display a decline of Cdx1 mRNA, which is mostly found in vivo in crypt cells. When implanted in nude mice, Cdx2-overexpressing cells produce larger tumors than control cells, and form glandular and villus-like structures. Laminin-1 is known to stimulate intestinal cell differentiation in vitro. In the present study, we demonstrate that the differentiating effect of laminin-1 coatings on Caco2-TC7 cells is accompanied by an upregulation of Cdx2. To further document this observation, we analyzed a series of Caco2 clones in which the production of laminin-α1 chain is differentially inhibited by antisense RNA. We found a positive correlation between the level of Cdx2 expression, that of endogenous laminin-α1 chain mRNA and that of sucrase-isomaltase expression in these cell lines. Taken together, these results suggest (a) that Cdx1 and Cdx2 homeobox genes play distinct roles in the intestinal epithelium, (b) that Cdx2 provokes pleiotropic effects triggering cells towards the phenotype of differentiated villus enterocytes, and (c) that Cdx2 expression is modulated by basement membrane components. Hence, we conclude that Cdx2 plays a key role in the extracellular matrix–mediated intestinal cell differentiation.

scholarly journals Downregulation of Reproductive Homeobox Gene 6 (Rhox6) Interferes with Male Germ Cell Differentiation.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 657-657
Author(s):  
Chang Liu ◽  
Paige Tsai ◽  
Ana Marie Garcia Rivera ◽  
Brandon Logeman ◽  
David J. Miller ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Germ Cell ◽  
Homeobox Gene ◽  
Male Germ Cell ◽  
Germ Cell Differentiation

scholarly journals Distinct metabolic pathways mediate regulatory T cell differentiation and function

2020 ◽  
Vol 223 ◽  
pp. 53-61
Author(s):  
Hisashi Hashimoto ◽  
Oliver McCallion ◽  
Rosalie W.M. Kempkes ◽  
Joanna Hester ◽  
Fadi Issa
Keyword(s):  
Cell Differentiation ◽  
T Cell ◽  
Regulatory T Cell ◽  
Metabolic Pathways ◽  
T Cell Differentiation ◽  
And Function

Alteration of homeobox gene expression by N-ras transformation of PA-1 human teratocarcinoma cells

1991 ◽  
Vol 11 (7) ◽  
pp. 3573-3583
Author(s):  
R Buettner ◽  
S O Yim ◽  
Y S Hong ◽  
E Boncinelli ◽  
M A Tainsky
Keyword(s):  
Cell Differentiation ◽  
Growth Regulation ◽  
Homeobox Genes ◽  
Constitutive Expression ◽  
Homeobox Gene ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
Transformed Cells ◽  
Teratocarcinoma Cell ◽  
Cell Clones

We used a series of cell clones from a human teratocarcinoma cell line, PA-1, to study the effect of transformation by an activated N-ras oncogene on the expression of genes involved in retinoic acid (RA)-induced differentiation and growth regulation. Recently, it has been shown that expression of human HOX 2 genes is sequentially activated by RA beginning from Hox 2.9 at the 3' end of the HOX 2 cluster (A. Simeone, D. Acampora, L. Arcioni, P. W. Andrews, E. Boncinelli, and F. Mavilio, Nature [London] 346:763-766, 1990). We now report that six different genes of the cluster HOX 1 are sequentially induced by RA in a similar temporal pattern, beginning with genes at the 3' end of the cluster. However, in N-ras-transformed cell clones, RA-induced expression of these homeobox genes is delayed. Hox 1.4 and Hox 1.3, genes abundantly induced in nontransformed clones after 3 days of RA treatment, are expressed in N-ras-transformed cells only after 10 days of RA treatment. At this time, the cells' growth is arrested at very high density, and no differentiated morphologic characteristics are observed. Constitutive expression of a transfected Hox 1.4 gene under the control of a simian virus 40 promotor leads to differentiated cell morphology similar to that of the RA-induced phenotype and restores the growth-inhibitory effects of RA in N-ras-transformed cells. These observations provide evidence that enhanced proliferation in N-ras-transformed cells compromises teratocarcinoma cell differentiation by a mechanism that transiently suppresses homeobox gene induction and implies a central role for homeobox genes in RA-induced cell differentiation. We conclude that stimulation of a putative growth factor signal pathway, associated with ras-induced proliferation, transiently suppresses the induction of transcription factors functionally involved in cell growth and differentiation.

Activation of the p38 MAP kinase is involved in the regulation of intestinal cell differentiation by the homeobox gene Cdx2

2001 ◽  
Vol 120 (5) ◽  
pp. A497
Author(s):  
Patrick Laprise ◽  
Mathieu Houde ◽  
Claude Asselin ◽  
Nathalie Rivard
Keyword(s):  
Cell Differentiation ◽  
Map Kinase ◽  
Homeobox Gene ◽  
P38 Map Kinase ◽  
Intestinal Cell

scholarly journals Aging-related upregulation of the homeobox gene caudal represses intestinal stem cell differentiation in Drosophila

PLoS Genetics ◽  
2021 ◽  
Vol 17 (7) ◽  
pp. e1009649
Author(s):  
Kun Wu ◽  
Yiming Tang ◽  
Qiaoqiao Zhang ◽  
Zhangpeng Zhuo ◽  
Xiao Sheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Progenitor Cells ◽  
Homeobox Gene ◽  
Stem Cell Differentiation ◽  
Janus Kinase ◽  
Gene Target ◽  
Age Related ◽  
Differentiation Efficiency

The differentiation efficiency of adult stem cells undergoes a significant decline in aged animals, which is closely related to the decline in organ function and age-associated diseases. However, the underlying mechanisms that ultimately lead to this observed decline of the differentiation efficiency of stem cells remain largely unclear. This study investigated Drosophila midguts and identified an obvious upregulation of caudal (cad), which encodes a homeobox transcription factor. This factor is traditionally known as a central regulator of embryonic anterior-posterior body axis patterning. This study reports that depletion of cad in intestinal stem/progenitor cells promotes quiescent intestinal stem cells (ISCs) to become activate and produce enterocytes in the midgut under normal gut homeostasis conditions. However, overexpression of cad results in the failure of ISC differentiation and intestinal epithelial regeneration after injury. Moreover, this study suggests that cad prevents intestinal stem/progenitor cell differentiation by modulating the Janus kinase/signal transducers and activators of the transcription pathway and Sox21a-GATAe signaling cascade. Importantly, the reduction of cad expression in intestinal stem/progenitor cells restrained age-associated gut hyperplasia in Drosophila. This study identified a function of the homeobox gene cad in the modulation of adult stem cell differentiation and suggested a potential gene target for the treatment of age-related diseases induced by age-related stem cell dysfunction.

scholarly journals Metabolomics analysis of poly(l-lactic acid) nanofibers' performance on PC12 cell differentiation

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Xiaoman Su ◽  
Yan Huang ◽  
Rong Chen ◽  
Yiwen Zhang ◽  
Meichen He ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Cell Differentiation ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Spin Coating ◽  
Metabolic Pathways ◽  
Bioinformatics Analysis ◽  
Neurite Length ◽  
Metabolite Profiles ◽  
Surface Morphologies

Abstract The aim of this article is to reveal the influence of aligned/random poly(l-lactic acid) (PLLA) nanofibers on PC12 cell differentiation from the perspective of metabolic level. First, three materials—PLLA aligned nanofibers (PLLA AF), PLLA random nanofibers (PLLA RF) and PLLA films (control)—were prepared by electrospinning and spin coating. Their surface morphologies were characterized. Subsequently, the cell viability, cell morphology and neurite length of PC12 cells on the surface of the three materials were evaluated, indicating more neurites in the PLLA RF groups but the longer average neurite length in the PLLA AF groups. Next, the metabolite profiles of PC12 cells cultured on the surface of the three nanofibers after 12 h, 24 h and 36 h showed that, compared with the control, 51, 48 and 31 types of differential metabolites were detected at the three time points among the AF groups, respectively; and 56, 45 and 41 types among the RF groups, respectively. Furthermore, the bioinformatics analysis of differential metabolites identified two pathways and three metabolites critical to PC12 cell differentiation influenced by the nanofibers. In addition, the verification experiment on critical metabolites and metabolic pathways were performed. The integrative analysis combining cytology, metabolomics and bioinformatics approaches revealed that though both PLLA AF and RF were capable of stimulating the synthesis of neurotransmitters, the PLLA AF were more beneficial for PC12 cell differentiation, whereas the PLLA RF were less effective.

scholarly journals Regulation of Retinal Cone Bipolar Cell Differentiation and Photopic Vision by the CVC Homeobox Gene Vsx1

2004 ◽  
Vol 14 (6) ◽  
pp. 530-536 ◽  
Author(s):  
Akihira Ohtoshi ◽  
Steven W Wang ◽  
Hidetaka Maeda ◽  
Shannon M Saszik ◽  
Laura J Frishman ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Bipolar Cell ◽  
Homeobox Gene ◽  
Photopic Vision ◽  
Retinal Cone
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