Faculty Opinions recommendation of Dynamics and function of Langerhans cells in vivo: dermal dendritic cells colonize lymph node areas distinct from slower migrating Langerhans cells.

2005 ◽  
Author(s):  
Andrea Sant
Keyword(s):  
Dendritic Cells ◽  
Lymph Node ◽  
Langerhans Cells ◽  
Dynamics And Function ◽  
And Function

scholarly journals Dynamics and Function of Langerhans Cells In Vivo

Immunity ◽  
2005 ◽  
Vol 22 (5) ◽  
pp. 643-654 ◽  
Author(s):  
Adrien Kissenpfennig ◽  
Sandrine Henri ◽  
Bertrand Dubois ◽  
Corinne Laplace-Builhé ◽  
Pierre Perrin ◽  
...  
Keyword(s):  
Langerhans Cells ◽  
Dynamics And Function ◽  
And Function

scholarly journals Inducible ablation of mouse Langerhans cells diminishes but fails to abrogate contact hypersensitivity

2005 ◽  
Vol 169 (4) ◽  
pp. 569-576 ◽  
Author(s):  
Clare L. Bennett ◽  
Erwin van Rijn ◽  
Steffen Jung ◽  
Kayo Inaba ◽  
Ralph M. Steinman ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Steady State ◽  
Diphtheria Toxin ◽  
Langerhans Cells ◽  
Contact Hypersensitivity ◽  
Relative Importance ◽  
Skin Immunity

Langerhans cells (LC) form a unique subset of dendritic cells (DC) in the epidermis but so far their in vivo functions in skin immunity and tolerance could not be determined, in particular in relation to dermal DC (dDC). Here, we exploit a novel diphtheria toxin (DT) receptor (DTR)/DT-based system to achieve inducible ablation of LC without affecting the skin environment. Within 24 h after intra-peritoneal injection of DT into Langerin-DTR mice LC are completely depleted from the epidermis and only begin to return 4 wk later. LC deletion occurs by apoptosis in the absence of inflammation and, in particular, the dDC compartment is not affected. In LC-depleted mice contact hypersensitivity (CHS) responses are significantly decreased, although ear swelling still occurs indicating that dDC can mediate CHS when necessary. Our results establish Langerin-DTR mice as a unique tool to study LC function in the steady state and to explore their relative importance compared with dDC in orchestrating skin immunity and tolerance.

scholarly journals In Vitro and In Vivo Effects of Ketamine on Generation and Function of Dendritic Cells

2011 ◽  
Vol 117 (3) ◽  
pp. 170-179 ◽  
Author(s):  
Jingxian Zeng ◽  
Shuxuan Xia ◽  
Wa Zhong ◽  
Jie Li ◽  
Liling Lin
Keyword(s):  
Dendritic Cells ◽  
And Function ◽  
In Vivo Effects

scholarly journals Decellularized Lymph Node Scaffolding as a Carrier for Dendritic Cells to Induce Anti-Tumor Immunity

Pharmaceutics ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 553 ◽  
Author(s):  
Hung-Jun Lin ◽  
Weu Wang ◽  
Yi-You Huang ◽  
Wei-Tsen Liao ◽  
Ting-Yu Lin ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Lymph Node ◽  
Antitumor Immunity ◽  
Cpg Oligodeoxynucleotides ◽  
Mhc Ii ◽  
Decellularized Extracellular Matrix ◽  
Histocompatibility Complex ◽  
Ecm Architecture ◽  
In Vivo Delivery

In recent decades, the decellularized extracellular matrix (ECM) has shown potential as a promising scaffold for tissue regeneration. In this study, an organic acid decellularized lymph node (dLN) was developed as a carrier for dendritic cells (DCs) to induce antitumor immunity. The dLNs were prepared by formic acid, acetic acid, or citric acid treatment. The results showed highly efficient removal of cell debris from the lymph node and great preservation of ECM architecture and biomolecules. In addition, bone marrow dendritic cells (BMDCs) grown preferably inside the dLN displayed the maturation markers CD80, CD86, and major histocompatibility complex (MHC)-II, and they produced high levels of interleukin (IL)-1β, IL-6, and IL-12 cytokines when stimulated with ovalbumin (OVA) and CpG oligodeoxynucleotides (CPG-ODN). In an animal model, the BMDC-dLN completely rejected the E.G7-OVA tumor. Furthermore, the splenocytes from BMDC-dLN-immunized mice produced more interferon gamma, IL-4, IL-6, and IL-2, and they had a higher proliferation rate than other groups when re-stimulated with OVA. Hence, BMDC-dLN could be a promising DC-based scaffold for in vivo delivery to induce potent antitumor immunity.

In Vivo Imaging of Lymph Node Migration of MNP- and 111In-Labeled Dendritic Cells in a Transgenic Mouse Model of Breast Cancer (MMTV-Ras)

2011 ◽  
Vol 14 (2) ◽  
pp. 183-196 ◽  
Author(s):  
Cristina Martelli ◽  
Manuela Borelli ◽  
Luisa Ottobrini ◽  
Veronica Rainone ◽  
Anna Degrassi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dendritic Cells ◽  
Lymph Node ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
In Vivo Imaging ◽  
Transgenic Mouse Model

Tracking Dendritic Cells In Vivo: Insights Into DC Biology and Function

2004 ◽  
Vol 29 (1-3) ◽  
pp. 069-080 ◽  
Author(s):  
Huiming Hon ◽  
Joshy Jacob
Keyword(s):  
Dendritic Cells ◽  
And Function

scholarly journals Localization of antigen on lymph node dendritic cells after exposure to the contact sensitizer fluorescein isothiocyanate. Functional and morphological studies.

1987 ◽  
Vol 166 (6) ◽  
pp. 1654-1667 ◽  
Author(s):  
S E Macatonia ◽  
S C Knight ◽  
A J Edwards ◽  
S Griffiths ◽  
P Fryer
Keyword(s):  
Dendritic Cells ◽  
Lymph Node ◽  
T Lymphocytes ◽  
Langerhans Cells ◽  
Fluorescein Isothiocyanate ◽  
Morphological Studies ◽  
Draining Lymph Node ◽  
Two Populations ◽  
Skin Painting

We have examined the cells involved in the development of contact sensitivity to FITC in CBA mice. After skin painting with antigen, the number of dendritic cells (DC) in the draining lymph nodes increased by 30 min, was maximal at 48 h, and returned to normal by 6 d. Derivation of some DC from Langerhans' cells of the skin was indicated from the presence of Birbeck granules observed in some DC isolated 24 h after skin painting. The DC acquired FITC and by 8 h there were two populations, one highly fluorescent and the other less fluorescent. The highly fluorescent cells were present between 8 h and 3 d after sensitization, and during this period the DC were potent at initiating primary proliferative responses of normal syngeneic T lymphocytes in vitro. Between days 3 and 5 the numbers of lymphocytes in the draining lymph node increased. During this period purified T lymphocytes did not express detectable levels of antigen, but enriched B cell populations expressed antigen transiently on day 1, 2, or 3 after exposure to antigen. The results showed that, during a 3-d period after exposure to antigen, DC expressed antigen and stimulated T cell proliferation. We speculate that low amounts of FITC binding selectively to veiled cells or lymph node DC in the first hours after exposure to antigen are not immunogenic but that Langerhans' cells acquire high levels of antigen, enter the nodes, and initiate immune responses.

Sign in / Sign up

Export Citation Format

Share Document