Faculty Opinions recommendation of Correlation between microdilution, E-test, and disk diffusion methods for antifungal susceptibility testing of posaconazole against Candida spp.

2006 ◽  
Author(s):  
Ana Espinel-Ingroff
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Disk Diffusion ◽  
Candida Spp

scholarly journals Antifungal susceptibility of clinically significant candida species by disk diffusion method

2021 ◽  
Vol 7 (2) ◽  
pp. 77-80
Author(s):  
Ajitha Reddy Edula
Keyword(s):  
Antifungal Agents ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Susceptibility Test ◽  
Disk Diffusion ◽  
Diffusion Method ◽  
Candida Spp ◽  
Disk Diffusion Method ◽  
Antifungal Susceptibility Test

Antifungal susceptibility of candida. To perform antifungal susceptibility testing on candida isolates by disk diffusion method & study its susceptibility pattern. The present study was conducted in the department of Microbiology in a tertiary care hospital in Hyderabad from January 2013 to June 2014, with prior approval of the Institutional Ethics Committee. The present study was designed to perform antifungal susceptibility test on Candida isolates by Disk Diffusion Method and study its susceptibility pattern. 102 Candida isolates were subjected to Antifungal susceptibility testing by Disk diffusion method using Mueller-Hinton Agar + 2% Glucose and 0.5 μg/mL Methylene Blue Dye (GMB) Mediumas per CLSI guidelines. : Antifungal susceptibility test shows that C. albicans is more susceptible to all the antifungal agents tested. Resistance to azole group of drugs was more pronounced in non-albicans candida spp. Voriconazole seemed to be superior to Fluconazole with a better susceptibility in the Fluconazole resistant strains also.Findings of the antifungal susceptibility test suggest that Candida spp., differ in their susceptibility to antifungal agents. Antifungal susceptibility testing of Candida isolates will be helpful in guiding physicians to select the appropriate antifungal drug so that therapeutic failures can be avoided thus decreasing patient morbidity and mortality.

scholarly journals Correlation between microdilution, Etest, and disk diffusion methods for antifungal susceptibility testing of fluconazole against Candida sp. blood isolates

2013 ◽  
Vol 46 (1) ◽  
pp. 106-107 ◽  
Author(s):  
Everardo Albuquerque Menezes ◽  
Antônio Alexandre de Vasconcelos Júnior ◽  
Maria Rozzelê Ferreira Ângelo ◽  
Maria da Conceição dos Santos Oliveira Cunha ◽  
Francisco Afrânio Cunha
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Disk Diffusion ◽  
Candida Sp

scholarly journals Antifungal susceptibility testing.

1993 ◽  
Vol 6 (4) ◽  
pp. 367-381 ◽  
Author(s):  
J H Rex ◽  
M A Pfaller ◽  
M G Rinaldi ◽  
A Polak ◽  
J N Galgiani
Keyword(s):  
Susceptibility Testing ◽  
Incubation Temperature ◽  
Clinical Laboratory ◽  
Antifungal Susceptibility ◽  
Inoculum Size ◽  
Antifungal Susceptibility Testing ◽  
National Committee ◽  
Candida Spp ◽  
Interlaboratory Variability ◽  
Future Work

Unlike antibacterial susceptibility testing, reliable antifungal susceptibility testing is still largely in its infancy. Many methods have been described, but they produce widely discrepant results unless such factors as pH, inoculum size, medium formulation, incubation time, and incubation temperature are carefully controlled. Even when laboratories agree upon a common method, interlaboratory agreement may be poor. As a result of numerous collaborative projects carried out both independently and under the aegis of the Subcommittee on Antifungal Susceptibility Testing of the National Committee for Clinical Laboratory Standards, the effects of varying these factors have been extensively studied and a standard method which minimizes interlaboratory variability during the testing of Candida spp. and Cryptococcus neoformans has been proposed. This review summarizes this work, reviews the strengths and weaknesses of the proposed susceptibility testing standard, and identifies directions for future work.

scholarly journals Correlation between Etest®, disk diffusion, and microdilution methods for antifungal susceptibility testing ofCandidaspecies from infection and colonization

2009 ◽  
Vol 23 (5) ◽  
pp. 324-330 ◽  
Author(s):  
Melyssa Negri ◽  
Mariana Henriques ◽  
Terezinha I.E. Svidzinski ◽  
Claudete Rodrigues Paula ◽  
Rosário Oliveira
Keyword(s):  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Disk Diffusion

scholarly journals Disk diffusion Method in Enriched Mueller Hinton agar for determining susceptibility of Candida isolates from various clinical specimens

2020 ◽  
Vol 28 (1) ◽  
pp. 28-33
Author(s):  
Nabeela Mahboob ◽  
Hasina Iqbal ◽  
Mushtaque Ahmed ◽  
Md Mehedi Hasan Magnet ◽  
Kazi Zulfiquer Mamun
Keyword(s):  
Methylene Blue ◽  
Amphotericin B ◽  
Candida Species ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Disk Diffusion ◽  
Clinical Specimens ◽  
Mueller Hinton ◽  
Mueller Hinton Agar

Background: Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases. Recently there is a serious concern with increased resistance of antifungal drugs and its consequences. Thus, identification of Candida and its antifungal susceptibility testing has a paramount significance in the management of Candidal infections. The aim of the study was to determine antifungal susceptibility pattern of Candida by Mueller-Hinton agar media supplemented with glucose and methylene blue for disk diffusion testing of fluconazole, miconazole, clotrimazole, amphotericin B and nystatin. Methods: A total of 35 Candida species was isolated from 2000 clinical specimens over 6 month’s period from July 2016 to December 2016. Growths on Blood agar and chromogenic agar were evaluated for colony appearance and microscopic examination. Antifungal susceptibility testing was performed by disk diffusion using Mueller-Hinton agar supplemented with glucose and methylene blue. Results: Candida species were more sensitive to clotrimazole (88.58%) and amphotericin B (88.58%) followed by nystatin ((77.14%), miconazole (74.29%) whereas fluconazole showed the highest level of resistance (60%). Conclusions: The increase in resistance to fluconazole is of serious concern as it is the most commonly used azole for candidiasis. The sensitivity profile of Candida isolates will be helpful to choose appropriate antifungal agents, thus decreasing patient’s morbidity and mortality. J Dhaka Medical College, Vol. 28, No.1, April, 2019, Page 28-33

scholarly journals Corrigendum: Growth kinetics in Candida spp.: Differences between species and potential impact on antifungal susceptibility testing as described by the EUCAST.

2019 ◽  
Vol 57 (5) ◽  
pp. e8-e10 ◽  
Author(s):  
María Ángeles Bordallo-Cardona ◽  
Carlos Sánchez-Carrillo ◽  
Patricia Muñoz ◽  
Emilio Bouza ◽  
Pilar Escribano ◽  
...  
Keyword(s):  
Growth Kinetics ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Candida Spp ◽  
Potential Impact

scholarly journals Evaluation of the “Dip Effect” Phenomenon in Antifungal Susceptibility Testing of Candida spp. against Echinocandins by Use of Gradient Concentration Strips

2015 ◽  
Vol 53 (11) ◽  
pp. 3654-3659 ◽  
Author(s):  
Maria Siopi ◽  
Marilena Tsala ◽  
Nikolaos Siafakas ◽  
Loukia Zerva ◽  
Joseph Meletiadis
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Candida Glabrata ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Antifungal Susceptibility Testing ◽  
Practical Approach ◽  
Candida Spp ◽  
Content Type ◽  
Inhibition Zones

The “dip effect” phenomenon complicates antifungal susceptibility testing with gradient concentration strips. Of 60Candidaisolates tested with the three echinocandins, this phenomenon was observed only for caspofungin with most (>90%)Candida albicans,Candida glabrata, andCandida tropicalisisolates and for isolates with CLSI MICs of ≤0.25 mg/liter. In order to facilitate MIC determination, a practical approach was developed using the inhibition zones at 32, 8, 2, and 1 mg/liter, increasing the agreement with the CLSI method >86%.

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