Faculty Opinions recommendation of Validation of an indirect ELISA based on a recombinant nucleocapsid protein of Rift Valley fever virus for the detection of IgG antibody in humans.

2007 ◽  
Author(s):  
Kouichi Morita
Keyword(s):  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Indirect Elisa ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Igg Antibody ◽  
Valley Fever ◽  
Recombinant Nucleocapsid Protein

scholarly journals Large-Scale International Validation of an Indirect ELISA Based on Recombinant Nucleocapsid Protein of Rift Valley Fever Virus for the Detection of IgG Antibody in Domestic Ruminants

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1651
Author(s):  
Janusz T. Pawęska ◽  
Petrus Jansen van Vuren ◽  
Veerle Msimang ◽  
Modu Moustapha Lô ◽  
Yaya Thiongane ◽  
...  
Keyword(s):  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Large Scale ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Valley Fever ◽  
Recombinant Nucleocapsid Protein

Diagnostic performance of an indirect enzyme-linked immunosorbent assay (I-ELISA) based on a recombinant nucleocapsid protein (rNP) of the Rift Valley fever virus (RVFV) was validated for the detection of the IgG antibody in sheep (n = 3367), goat (n = 2632), and cattle (n = 3819) sera. Validation data sets were dichotomized according to the results of a virus neutralization test in sera obtained from RVF-endemic (Burkina Faso, Democratic Republic of Congo, Mozambique, Senegal, Uganda, and Yemen) and RVF-free countries (France, Poland, and the USA). Cut-off values were defined using the two-graph receiver operating characteristic analysis. Estimates of the diagnostic specificity of the RVFV rNP I-ELISA in animals from RVF-endemic countries ranged from 98.6% (cattle) to 99.5% (sheep) while in those originating from RVF-free countries, they ranged from 97.7% (sheep) to 98.1% (goats). Estimates of the diagnostic sensitivity in ruminants from RVF-endemic countries ranged from 90.7% (cattle) to 100% (goats). The results of this large-scale international validation study demonstrate the high diagnostic accuracy of the RVFV rNP I-ELISA. Standard incubation and inactivation procedures evaluated did not have an adverse effect on the detectable levels of the anti-RVFV IgG in ruminant sera and thus, together with recombinant antigen-based I-ELISA, provide a simple, safe, and robust diagnostic platform that can be automated and carried out outside expensive bio-containment facilities. These advantages are particularly important for less-resourced countries where there is a need to accelerate and improve RVF surveillance and research on epidemiology as well as to advance disease control measures.

scholarly journals Characterization of RNA aptamers directed against the nucleocapsid protein of Rift Valley fever virus

2012 ◽  
Vol 93 (3) ◽  
pp. 330-339 ◽  
Author(s):  
Mary Ellenbecker ◽  
Leila Sears ◽  
Ping Li ◽  
Jean-Marc Lanchy ◽  
J. Stephen Lodmell
Keyword(s):  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Rna Aptamers ◽  
Valley Fever

Optical fiber immunosensor for the detection of IgG antibody to Rift Valley fever virus in humans

2007 ◽  
Vol 146 (1-2) ◽  
pp. 327-334 ◽  
Author(s):  
A. Sobarzo ◽  
J.T. Paweska ◽  
S. Herrmann ◽  
T. Amir ◽  
R.S. Marks ◽  
...  
Keyword(s):  
Optical Fiber ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Igg Antibody ◽  
Valley Fever

Generation and application of monoclonal antibodies against Rift Valley fever virus nucleocapsid protein NP and glycoproteins Gn and Gc

2013 ◽  
Vol 159 (3) ◽  
pp. 535-546 ◽  
Author(s):  
Susanne Jäckel ◽  
Martin Eiden ◽  
Malte Dauber ◽  
Anne Balkema-Buschmann ◽  
Alejandro Brun ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever

scholarly journals The Nucleocapsid Protein of Rift Valley Fever Virus Is a Potent Human CD8+ T Cell Antigen and Elicits Memory Responses

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e59210 ◽  
Author(s):  
Weidong Xu ◽  
Douglas M. Watts ◽  
Margaret C. Costanzo ◽  
Xiaolei Tang ◽  
Leon A. Venegas ◽  
...  
Keyword(s):  
T Cell ◽  
Rift Valley ◽  
Nucleocapsid Protein ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Cd8 T Cell ◽  
Fever Virus ◽  
Valley Fever ◽  
Cell Antigen

A novel indirect ELISA based on glycoprotein Gn for the detection of IgG antibodies against Rift Valley fever virus in small ruminants

2013 ◽  
Vol 95 (2) ◽  
pp. 725-730 ◽  
Author(s):  
S. Jäckel ◽  
M. Eiden ◽  
A. Balkema-Buschmann ◽  
M. Ziller ◽  
P. Jansen van Vuren ◽  
...  
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Indirect Elisa ◽  
Rift Valley Fever Virus ◽  
Small Ruminants ◽  
Fever Virus ◽  
Igg Antibodies ◽  
Valley Fever
Sign in / Sign up

Export Citation Format

Share Document