Faculty Opinions recommendation of Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage.

2016 ◽  
Author(s):  
Mohan Balasubramanian ◽  
Ting Gang Chew ◽  
Ramanujam Srinivasan
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Stage ◽  
Rhoa Activation ◽  
Spindle Position

scholarly journals Local RhoA Activation Induces Cytokinetic Furrows Independent of Spindle Position and Cell Cycle Stage

2016 ◽  
Author(s):  
Elizabeth Wagner ◽  
Michael Glotzer
Keyword(s):  
Cell Cycle ◽  
Mammalian Cells ◽  
Contractile Ring ◽  
Rhoa Activity ◽  
Rhoa Activation ◽  
Ring Assembly ◽  
Interphase Cells ◽  
A Site ◽  
Spindle Position ◽  
Rapid Activation

Cytokinetic cleavage furrows assemble during anaphase at a site that is dictated by the position of the spindle. The GTPase RhoA promotes contractile ring assembly and furrow ingression during cytokinesis. While many factors that regulate RhoA during cytokinesis have been characterized, the spatiotemporal regulatory logic remains undefined. It is not known whether a local zone of RhoA activity is sufficient to induce furrow formation or whether the spindle modulates furrow assembly through other pathways. Similarly, it is not known whether the entire cortex is responsive to RhoA, nor whether contractile ring assembly is cell cycle regulated. Here, we have developed an optogenetic probe to gain tight spatial and temporal control of RhoA activity in mammalian cells and demonstrate that cytokinetic furrowing is primarily regulated at the level of RhoA activation. Light-mediated recruitment of a RhoGEF domain to the plasma membrane leads to rapid activation of RhoA, leading to assembly of cytokinetic furrows that partially ingress. Furthermore, furrow formation in response to RhoA activation is not spatially or temporally restricted. RhoA activation is sufficient to generate furrows at both the cell equator and at cell poles, in both metaphase and anaphase. Remarkably, furrow formation can be initiated in rounded interphase cells, but not adherent cells. These results indicate RhoA activation is sufficient to induce assembly of functional contractile rings and that cell rounding facilitates furrow formation.

scholarly journals Local RhoA activation induces cytokinetic furrows independent of spindle position and cell cycle stage

2016 ◽  
Vol 213 (6) ◽  
pp. 641-649 ◽  
Author(s):  
Elizabeth Wagner ◽  
Michael Glotzer
Keyword(s):  
Cell Cycle ◽  
Mammalian Cells ◽  
Adherent Cells ◽  
Contractile Ring ◽  
Rhoa Activity ◽  
Rhoa Activation ◽  
Rapid Induction ◽  
Ring Assembly ◽  
Interphase Cells ◽  
Spindle Position

The GTPase RhoA promotes contractile ring assembly and furrow ingression during cytokinesis. Although many factors that regulate RhoA during cytokinesis have been characterized, the spatiotemporal regulatory logic remains undefined. We have developed an optogenetic probe to gain tight spatial and temporal control of RhoA activity in mammalian cells and demonstrate that cytokinetic furrowing is primarily regulated at the level of RhoA activation. Light-mediated recruitment of a RhoGEF domain to the plasma membrane leads to rapid induction of RhoA activity, leading to assembly of cytokinetic furrows that partially ingress. Furthermore, furrow formation in response to RhoA activation is not temporally or spatially restricted. RhoA activation is sufficient to generate furrows at both the cell equator and cell poles, in both metaphase and anaphase. Remarkably, furrow formation can be initiated in rounded interphase cells, but not adherent cells. These results indicate that RhoA activation is sufficient to induce assembly of functional contractile rings and that cell rounding facilitates furrow formation.

scholarly journals The Number of Lipid Droplets in the Fission Yeast S. pombe is a Function of the Cell Cycle Stage

2011 ◽  
Vol 100 (3) ◽  
pp. 89a ◽  
Author(s):  
Allan Long ◽  
Anna Manneschmidt ◽  
Rose Dortch ◽  
Robert Verbruggie ◽  
Paul Dalhaimer
Keyword(s):  
Cell Cycle ◽  
Fission Yeast ◽  
Lipid Droplets ◽  
Cell Cycle Stage

Presence of Clustered GM1 Ganglioside in the Membrane of Endometrial Mesenchymal Stem Cells is Dependent on Cell Cycle Stage

2021 ◽  
Vol 15 (2) ◽  
pp. 120-126
Author(s):  
V. I. Chubinskiy-Nadezhdin ◽  
M. A. Shilina ◽  
A. V. Sudarikova ◽  
O. G. Lyublinskaya ◽  
Yu. A. Negulyaev ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Gm1 Ganglioside ◽  
Cell Cycle Stage

59 SYNCHRONIZATION OF CELL CYCLE STAGE OF BUFFALO (BUBALUS BUBALIS) FETAL FIBROBLAST CELLS BY DIFFERENT TREATMENTS

2011 ◽  
Vol 23 (1) ◽  
pp. 135
Author(s):  
N. L. Selokar ◽  
A. George ◽  
A. P. Saha ◽  
R. Sharma ◽  
M. Muzaffar ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Bubalus Bubalis ◽  
Serum Starvation ◽  
Fibroblast Cells ◽  
Cloning Efficiency ◽  
Fetal Fibroblast ◽  
Cell Cycle Stage ◽  
Cell Cycle Synchronization ◽  
Donor Cells ◽  
Major Factors

Cell cycle stage of donor cells significantly influences the cloning efficiency during SCNT. Donor cells in G1/G0 stage have better capability to undergo nuclear reprogramming following transfer to an unfertilized oocyte. The lack of availability of cells synchronized at G1/G0 stage is one of the major factors limiting cloning efficiency in buffalo. The aim of this study was to compare the efficacy of various methods for cell cycle synchronization of buffalo fetal fibroblast cells for SCNT. Cells isolated from fetus, 2 to 3 months old, were cultured in DMEM + 10% FBS. The primary culture was sub-cultured 8 to 10 times. For cell cycle synchronization, the cells were cultured to 1) 60 to 70% confluence (controls), 2) 60 to 70% confluence followed by serum starvation (DMEM + 0.5% FBS) for 24 h (serum starved), 3), full confluence followed by culture for additional 3 to 5 days (full confluent), 4) full confluence followed by serum starvation (DMEM + 0.5% FBS) for 24 h (full confluent+serum starved) and 5) 60 to 70% confluence followed by treatment with roscovitine (10, 20, or 30 μM) for 24 h. The synchronization efficiency was examined by propidium iodide staining followed by analysis of DNA content using flow cytometry and the data were analysed by 1-way ANOVA followed by Fisher’s l.s.d. test after arcsine transformation. The percentage of cells in G0/G1 phase of cell cycle was significantly higher (P < 0.05) in the full confluent+serum starved and roscovitine treated (20 or 30 μM) groups than that in the full confluent group and that treated with 10 μM roscovitine which, in turn, was higher (P < 0.05) than that in the serum starved and control groups. These results suggest that buffalo fetal fibroblast cells can be synchronized by roscovitine treatment or by serum starvation of fully confluent cell cultures to obtain a high proportion of cells in G0/G1 stage for SCNT. Table 1.Buffalo skin fibroblast cells at various stages following different treatments for cell cycle synchronization Supported by grant No. 1(5)/2007-NAIP from ICAR, India.

Histone proteins in HeLa S3 cells are synthesized in a cell cycle stage specific manner

Science ◽  
1982 ◽  
Vol 215 (4533) ◽  
pp. 683-685 ◽  
Author(s):  
F Marashi ◽  
L Baumbach ◽  
R Rickles ◽  
F Sierra ◽  
J. Stein ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Histone Proteins ◽  
Cell Cycle Stage ◽  
Specific Manner ◽  
A Cell ◽  
Hela S3 ◽  
Hela S3 Cells
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