Histone proteins in HeLa S3 cells are synthesized in a cell cycle stage specific manner

Science ◽  
1982 ◽  
Vol 215 (4533) ◽  
pp. 683-685 ◽  
Author(s):  
F Marashi ◽  
L Baumbach ◽  
R Rickles ◽  
F Sierra ◽  
J. Stein ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Histone Proteins ◽  
Cell Cycle Stage ◽  
Specific Manner ◽  
A Cell ◽  
Hela S3 ◽  
Hela S3 Cells

scholarly journals Connexin 43 Interacts with Zona Occludens-1 and -2 Proteins in a Cell Cycle Stage-specific Manner

2005 ◽  
Vol 280 (34) ◽  
pp. 30416-30421 ◽  
Author(s):  
Deepika Singh ◽  
Joell L. Solan ◽  
Steven M. Taffet ◽  
Ronald Javier ◽  
Paul D. Lampe
Keyword(s):  
Cell Cycle ◽  
Connexin 43 ◽  
Cell Cycle Stage ◽  
Specific Manner ◽  
A Cell ◽  
Zona Occludens

X-ray microanalysis of HeLa S3 cells. II. Analysis of elemental levels during the cell cycle

1983 ◽  
Vol 62 (1) ◽  
pp. 339-350
Author(s):  
A. Warley ◽  
J. Stephen ◽  
A. Hockaday ◽  
T.C. Appleton
Keyword(s):  
Cell Cycle ◽  
Monovalent Cation ◽  
The Other ◽  
G1 Phase ◽  
Sodium Potassium ◽  
X Ray ◽  
Cation Concentration ◽  
Cell Compartments ◽  
Hela S3 ◽  
Hela S3 Cells

HeLa S3 cells were synchronized using hydroxyurea. Cryoultramicrotomy and X-ray microanalysis were used to study changes occurring in concentrations of elements during the cell cycle of the synchronized cells. Three subcellular compartments were studied: cytoplasm, nucleus and nucleolus. Potassium concentrations showed little fluctuation in all of the cell compartments during the cell cycle. Sodium concentrations increased during S. and M phases, returning to lower levels in the G1 phase. Chlorine concentrations were highest during the S and G2 phases. At all stages of the cell cycle respective concentrations of potassium, sodium, sulphur and chlorine were similar in the cytoplasm and nucleus. Concentrations of phosphorus increased in the nucleus during S, G2 and M, and also showed fluctuations in the nucleolus during the cycle; these were not seen in the cytoplasm. In S, M and M/G1 sodium concentrations were highest in the nucleolus compared with the other compartments. In the cytoplasm these changes resulted in an increase in total monovalent cation concentration (i.e. sodium + potassium) during S, G2 and M, which returned to base levels after mitosis. This increase in monovalent cation concentration is due almost entirely to the increase in sodium, with little change occurring in the concentration of potassium.

Human GST5.8, Expressed in a Cell Cycle Specific Manner, May Offer a Method to Alter 4-Hydroxynonenal Concentrations of Philadelphia-Positive Chronic Myelogenous Leukemia Cells.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4260-4260
Author(s):  
Kathryn L. Leake ◽  
Sanjay Awasthi ◽  
Manmeet Mangat
Keyword(s):  
Cell Cycle ◽  
Lipid Peroxidation ◽  
Chronic Myelogenous Leukemia ◽  
K562 Cells ◽  
Myelogenous Leukemia ◽  
Lipid Peroxidation Product ◽  
Specific Manner ◽  
A Cell ◽  
Peroxidation Product ◽  
Time Point

Abstract Abstract 4260 Background Chronic Myelogenous Leukemia studies in K562 cell line, obtained from the pleural effusion of a CML patient in blastic crisis, initially focused upon chromosomal translocations such as del(22) (q12) and t(15;17)(q21;q24). {{Lozzio,C.B. 1975}} In a 2001 Phase I Clinical Trial, STI-571, a tyrosine kinase inhibitor which treats the BCR/Abl fusion protein formed due to t(9;22)(q34;q11) found in the majority of CML patients effectively ceased proliferation of BCR-Abl expressing hematopoietic cells.{{Brian J. Druker, M.D., 2001}} However, recent studies demonstrate that Chronic Myelogenous Leukemia persists in patients exhibiting differing chromosomal abnormalities. {{Jamshid S Khorashad, MD, 2008}} Therefore, we examined methods for inducing differentiation in K562 cells to pursue the senescence of leukemic blasts beyond proliferation in heterogeneic CML populations. Using 20 uM 4-hydroxynonenal, a lipid peroxidation product, Cheng successfully produced differentiated K562 cells. {{Cheng,J.Z. 1999}} Our studies confirm this; however 20 um-HNE-treated K562 cells also aggregate. Here, we performed titration studies examining extremely low concentrations of 4-HNE and its correlation with K562 morphology to investigate if low-HNE-containing cells remained premature blasts. Barrera also demonstrated inhibition of cell proliferation, as well as the downregulation of c-myc expression in K562 cells, by HNE in a dose-dependent manner. {{Barrera,G. 2004}} However, he reported that these effects were transient and disappeared within 6–8 hours following HNE administration. Because levels of aldehydes, such as 4-HNE, formed by lipid peroxidation are regulated through conjugation to glutathione, we investigated glutathione-S-transferase activity in K562 cells, which has been shown to exhibit extremely high specificity for 4-hydroxynonenal and is known to reduce 4-HNE concentrations in tissue cells. {{Cheng,J.Z. 1999}} Hypothesis Human GST5.8 exhibits the highest specific activity of the glutathione-S-transferases toward lipid peroxidation product, 4-HNE as shown in tissue. {{Singhal,S.S. 1999}} So, we observed if higher concentrations of the enzymatic activity occurred at specific points in the K562 cell cycle, affecting HNE concentrations and contributing to cell cycle phases such as G0/G1 or S phase. Methods We examined human GST5.8 levels in K562 cells following synchronized release from arrest with sodium butyrate. In addition, we performed morphological analysis of HNE effects on K562 cells via Giemsa stained negative controls versus titrations of HNE-treated K562 cells (at 0.5-20 uM) for 2 hours. Also, Western Blots and FACS analyses identifying time points of highest human GST5.8 expression were produced. Results Densitometric analysis of hGST5.8 expression by time point revealed that human GST5.8 is present in K562 cells and that the enzymatic concentration peaks at 0 hr to 30 minutes following release from arrest or the G0/G1 time point. Our results confirmed that 20 um HNE causes differentiation, aggregation, and apoptosis in K562 cells. We also showed 0.5 uM concentration produces differentiation but very minimal aggregation. Conclusions Because human GST5.8 peaks during the G0/G1 time point, causing a reduction of the 4-hydroxynonenal concentration; it is reasonable to conclude that hGST5.8 may rise during G0/G1 in order to lower HNE concentrations as a dedifferentiation mechanism in normal cell cycle events. Here, we have demonstrated that human GST5.8 is expressed in a cell-cycle specific manner. Future studies involve transfection of hGST5.8 antisense into K562 to learn if consistent induction of differentiation is possible. Taken together, these studies indicate that hGST5.8 modulation may hold promise as a method to control 4-hydroxynonenal levels and thus differentiation in Ph(+) Chronic Myelogenous Leukemia cells. Disclosures: No relevant conflicts of interest to declare.

The plant homologue of MAP kinase is expressed in a cell cycle-dependent and organ-specific manner

1993 ◽  
Vol 3 (4) ◽  
pp. 611-617 ◽  
Author(s):  
Claudia Jonak ◽  
Aniko Páy ◽  
Laszlo Börge ◽  
Heribert Hirt ◽  
Erwin Heberle-Bors
Keyword(s):  
Cell Cycle ◽  
Map Kinase ◽  
Specific Manner ◽  
Organ Specific ◽  
A Cell ◽  
Cycle Dependent ◽  
Plant Homologue

Deoxyribonuclease II activity in relation to cell cycle in synchronized HeLa S3 cells

1973 ◽  
Vol 52 (2) ◽  
pp. 556-561 ◽  
Author(s):  
Hanoch Slor ◽  
Haim Bustan ◽  
Tama Lev
Keyword(s):  
Cell Cycle ◽  
Hela S3 ◽  
Hela S3 Cells

scholarly journals Cell cycle dynamics of human pluripotent stem cells primed for differentiation

2019 ◽  
Author(s):  
Anna Shcherbina ◽  
Jingling Li ◽  
Cyndhavi Narayanan ◽  
William Greenleaf ◽  
Anshul Kundaje ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Expression Patterns ◽  
Human Pluripotent Stem Cells ◽  
Specific Manner ◽  
A Cell ◽  
Cell Cycle Dynamics ◽  
Differentiation Gene ◽  
Cycle Indicator

Understanding the molecular properties of the cell cycle of human pluripotent stem cells (hPSCs) is critical for effectively promoting differentiation. Here, we use the Fluorescence Ubiquitin Cell Cycle Indicator (FUCCI) system adapted into hPSCs and perform RNA-sequencing on cell cycle sorted hPSCs primed and unprimed for differentiation. Gene expression patterns of signaling factors and developmental regulators change in a cell cycle-specific manner in cells primed for differentiation without altering genes associated with pluripotency. Furthermore, we identify an important role for PI3K signaling in regulating the early transitory states of hPSCs towards differentiation.

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