Faculty Opinions recommendation of Dissociation between brown adipose tissue 18F-FDG uptake and thermogenesis in uncoupling protein 1 deficient mice.

2017 ◽  
Author(s):  
Michael Symonds
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Uncoupling Protein ◽  
Uncoupling Protein 1 ◽  
Fdg Uptake ◽  
Brown Adipose ◽  
18F Fdg ◽  
Deficient Mice

scholarly journals Dissociation Between Brown Adipose Tissue 18 F-FDG Uptake and Thermogenesis in Uncoupling Protein 1–Deficient Mice

2017 ◽  
Vol 58 (7) ◽  
pp. 1100-1103 ◽  
Author(s):  
Mohammed K. Hankir ◽  
Mathias Kranz ◽  
Susanne Keipert ◽  
Juliane Weiner ◽  
Sille G. Andreasen ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Uncoupling Protein ◽  
Uncoupling Protein 1 ◽  
Fdg Uptake ◽  
Brown Adipose ◽  
Deficient Mice

scholarly journals Brown adipose tissue lipoprotein and glucose disposal is not determined by thermogenesis in uncoupling protein 1-deficient mice

2020 ◽  
Vol 61 (11) ◽  
pp. 1377-1389 ◽  
Author(s):  
Alexander W. Fischer ◽  
Janina Behrens ◽  
Frederike Sass ◽  
Christian Schlein ◽  
Markus Heine ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Cold Exposure ◽  
Uncoupling Protein ◽  
Surrogate Marker ◽  
Glucose Disposal ◽  
Uncoupling Protein 1 ◽  
Adipose Tissues ◽  
Brown Adipose ◽  
Deficient Mice

Adaptive thermogenesis is highly dependent on uncoupling protein 1 (UCP1), a protein expressed by thermogenic adipocytes present in brown adipose tissue (BAT) and white adipose tissue (WAT). Thermogenic capacity of human and mouse BAT can be measured by positron emission tomography-computed tomography quantifying the uptake of 18F-fluodeoxyglucose or lipid tracers. BAT activation is typically studied in response to cold exposure or treatment with β-3-adrenergic receptor agonists such as CL316,243 (CL). Currently, it is unknown whether cold-stimulated uptake of glucose or lipid tracers is a good surrogate marker of UCP1-mediated thermogenesis. In metabolic studies using radiolabeled tracers, we found that glucose uptake is increased in mildly cold-activated BAT of Ucp1−/− versus WT mice kept at subthermoneutral temperature. Conversely, lower glucose disposal was detected after full thermogenic activation achieved by sustained cold exposure or CL treatment. In contrast, uptake of lipoprotein-derived fatty acids into chronically activated thermogenic adipose tissues was substantially increased in UCP1-deficient mice. This effect is linked to higher sympathetic tone in adipose tissues of Ucp1−/− mice, as indicated by elevated levels of thermogenic genes in BAT and WAT. Thus, glucose and lipoprotein handling does not necessarily reflect UCP1-dependent thermogenic activity, but especially lipid uptake rather mirrors sympathetic activation of adipose tissues.

Determinants of Physiologic 18F-FDG Uptake in Brown Adipose Tissue in Sequential PET/CT Examinations

2010 ◽  
Vol 13 (5) ◽  
pp. 1029-1035 ◽  
Author(s):  
Leonardo Pace ◽  
Emanuele Nicolai ◽  
Domenico D’Amico ◽  
Francesco Ibello ◽  
Anna Maria Della Morte ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Fdg Uptake ◽  
Brown Adipose ◽  
Pet Ct ◽  
18F Fdg

scholarly journals Sleep duration and quality are not associated with brown adipose tissue volume or activity—as determined by 18F-FDG uptake, in young, sedentary adults

SLEEP ◽  
2019 ◽  
Vol 42 (12) ◽  
Author(s):  
Francisco M Acosta ◽  
Guillermo Sanchez-Delgado ◽  
Borja Martinez-Tellez ◽  
Jairo H Migueles ◽  
Francisco J Amaro-Gahete ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Sleep Quality ◽  
Brown Adipose Tissue ◽  
Sleep Duration ◽  
Sleep Disturbances ◽  
Sleep Regulation ◽  
Clinical Trial Registration ◽  
Fdg Uptake ◽  
Brown Adipose ◽  
18F Fdg

Abstract Study Objectives Short sleep duration and sleep disturbances have been related to obesity and metabolic disruption. However, the behavioral and physiological mechanisms linking sleep and alterations in energy balance and metabolism are incompletely understood. In rodents, sleep regulation is closely related to appropriate brown adipose tissue (BAT) thermogenic activity, but whether the same is true in humans has remained unknown. The present work examines whether sleep duration and quality are related to BAT volume and activity (measured by 18F-FDG) and BAT radiodensity in humans. Methods A total of 118 healthy adults (69% women, 21.9 ± 2.2 years, body mass index: 24.9 ± 4.7 kg/m2) participated in this cross-sectional study. Sleep duration and other sleep variables were measured using a wrist-worn accelerometer for seven consecutive days for 24 hours per day. The Pittsburgh Sleep Quality Index was used to assess sleep quality. All participants then underwent a personalized cold exposure to determine their BAT volume, activity, and radiodensity (a proxy of the intracellular triglyceride content), using static positron emission tomography combined with computed tomography (PET/CI) scan. Results Neither sleep duration nor quality was associated with BAT volume or activity (the latter represented by the mean and peak standardized 18F-FDG uptake values) or radiodensity (all p > .1). The lack of association remained after adjusting the analyses for sex, date of PET/CT, and body composition. Conclusions Although experiments in rodent models indicate a strong relationship to exist between sleep regulation and BAT function, it seems that sleep duration and quality may not be directly related to the BAT variables examined in the present work. Clinical Trial Registration NCT02365129 (ClinicalTrials.gov).

scholarly journals Uncoupling protein 1 binds one nucleotide per monomer and is stabilized by tightly bound cardiolipin

2015 ◽  
Vol 112 (22) ◽  
pp. 6973-6978 ◽  
Author(s):  
Yang Lee ◽  
Chrissie Willers ◽  
Edmund R. S. Kunji ◽  
Paul G. Crichton
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Uncoupling Protein ◽  
Size Exclusion ◽  
Uncoupling Protein 1 ◽  
Mitochondrial Carriers ◽  
Mitochondrial Inner Membrane ◽  
Brown Adipose ◽  
Integral Role ◽  
Covalent Chromatography

Uncoupling protein 1 (UCP1) catalyzes fatty acid-activated, purine nucleotide-sensitive proton leak across the mitochondrial inner membrane of brown adipose tissue to produce heat, and could help combat obesity and metabolic disease in humans. Studies over the last 30 years conclude that the protein is a dimer, binding one nucleotide molecule per two proteins, and unlike the related mitochondrial ADP/ATP carrier, does not bind cardiolipin. Here, we have developed novel methods to purify milligram amounts of UCP1 from native sources by using covalent chromatography that, unlike past methods, allows the protein to be prepared in defined conditions, free of excess detergent and lipid. Assessment of purified preparations by TLC reveal that UCP1 retains tightly bound cardiolipin, with a lipid phosphorus content equating to three molecules per protein, like the ADP/ATP carrier. Cardiolipin stabilizes UCP1, as demonstrated by reconstitution experiments and thermostability assays, indicating that the lipid has an integral role in the functioning of the protein, similar to other mitochondrial carriers. Furthermore, we find that UCP1 is not dimeric but monomeric, as indicated by size exclusion analysis, and has a ligand titration profile in isothermal calorimetric measurements that clearly shows that one nucleotide binds per monomer. These findings reveal the fundamental composition of UCP1, which is essential for understanding the mechanism of the protein. Our assessment of the properties of UCP1 indicate that it is not unique among mitochondrial carriers and so is likely to use a common exchange mechanism in its primary function in brown adipose tissue mitochondria.

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