Comparison of a new hydro-surgical technique to traditional methods for the preparation of full-thickness skin grafts from canine cadaveric skin and report of a single clinical case

SummaryObjective: To compare the hydro-surgical technique to traditional techniques for removal of subcutaneous tissue in the preparation of full-thickness skin grafts.Study design: Ex vivo experimental study and a single clinical case report.Sample population: Four canine cadavers and a single clinical case.Methods: Four sections of skin were harvested from the lateral flank of recently euthanatized dogs. Traditional preparation methods used included both a blade or scissors technique, each of which were compared to the hydro-surgical technique individually. Preparation methods were compared based on length of time for removal of the subcutaneous tissue from the graft, histologic grading, and measurable thickness as compared to an untreated sample.Results: The hydro-surgical technique had the shortest skin graft preparation time as compared to traditional techniques (p = 0.002). There was no significant difference in the histological grading or measurable subcutaneous thickness between skin specimens.Clinical significance: The hydro-surgical technique provides a rapid, effective debridement of subcutaneous tissue in the preparation of full-thickness skin grafts. There were not any significant changes in histological grade and subcutaneous tissue remaining among all treatment types. Additionally the hydro-surgical technique was successfully used to prepare a full-thickness meshed free skin graft in the reconstruction of a traumatic medial tarsal wound in a dog.Presented at the ACVS symposium during the resident seminar, Seattle, Washington,USA on October 22, 2010.

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The Challenges of Skin Graft Lining in Nasal Reconstruction: Comment on “Full-Thickness Skin Grafts and Quilting Sutures for Reconstruction of Internal Nasal Lining”

Facial Plastic Surgery ◽

10.1055/s-0040-1717058 ◽

2021 ◽

Vol 37 (03) ◽

pp. 411-411

Author(s):

Yihao Xu ◽

Lehao Wu ◽

Fei Fan

Keyword(s):

Skin Graft ◽

Nasal Reconstruction ◽

Skin Grafts ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin

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Eyelid Reconstruction with Full Thickness Skin Grafts After Carcinoma Excision

Folia Medica ◽

10.1515/folmed-2016-0006 ◽

2016 ◽

Vol 58 (1) ◽

pp. 42-47 ◽

Cited By ~ 7

Author(s):

Zornitsa I. Zlatarova ◽

Binna N. Nenkova ◽

Ekaterina B. Softova

Keyword(s):

Skin Graft ◽

Skin Grafts ◽

Full Thickness ◽

Full Thickness Skin Graft ◽

Eyelid Reconstruction ◽

Full Thickness Skin ◽

Thickness Skin ◽

Additional Surgery ◽

Aesthetic Results ◽

The Aesthetic

AbstractBackground: Various techniques have been proposed for reconstruction of the eyelid anterior lamella after carcinoma excision: among these are the transposition of skin flaps, and full-thickness skin grafts or combination of these two.Aim: To present our experience in eyelid reconstruction with full-thickness skin grafts and to assess the aesthetic and functional outcomes.Patients and methods: The present retrospective study included 39 patients (20 males, 19 females, mean age 71 yrs) with surgically excised eyelid carcinoma, followed by reconstruction using full-thickness skin grafts. The patients were treated between 2005 and 2014. Parameters recorded were patient demographics, histological classification of malignancy, tumor localization and size, postoperative defect size. In cases of large full-thickness lower lid defect Hughes tarsoconjunctival flap was used for reconstruction of posterior lamella. Full-thickness skin grafts donor sites included upper eyelid, preauricular area and inner brachial area. We appraised the grafts viability one week after surgery and the aesthetic results - 6 months after surgery by the graft colour and lid position.Results: In 95% of the cases the skin grafts were viable. The full-thickness skin graft (FTSG) failed in two patients because of subcutaneous haematoma. There were a few early postoperative complications including graft hypertrophy, graft contraction, and partial graft failure, which were managed without additional surgery. All 39 patients had normal postoperative lid function. All 39 had either good (14) or excellent (25) cosmetic results.Conclusions: Our findings suggest that full-thickness skin graft is a good choice in periocular reconstructive surgery after carcinoma excision. The surgical technique is easy to perform producing proper functional and aesthetic results.

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A Comparative Study of Graft-Take Between Tie-Over Dressing and Dressing with Multiple Quilting in Full Thickness Skin Graft

Bangladesh Journal of Plastic Surgery ◽

10.3329/bdjps.v3i2.18249 ◽

2014 ◽

Vol 3 (2) ◽

pp. 38-44

Author(s):

Md Maruful Islam ◽

Imran Choudhury ◽

Md Nashir Uddin ◽

Lutfar Kader Lenin ◽

Rayhana Awwal ◽

...

Keyword(s):

Comparative Study ◽

Plastic Surgery ◽

Skin Graft ◽

Full Thickness ◽

Full Thickness Skin Graft ◽

Recipient Site ◽

Full Thickness Skin ◽

Graft Take ◽

Thickness Skin ◽

Significant Difference

A skin graft is the simplest way of reconstructing an area of skin loss. The graft must acquire blood supply from the wound bed and taken by the recipient site. The aim of the present study was to compare the outcome of graft-take of full thickness skin graft by tie-over dressing versus multiple quilting and simple dressing in face and neck region. This prospective, interventional, comparative study was conducted in the Department of Plastic Surgery, Dhaka Medical College Hospital from January 2009 to December 2010 (2 years). A total number of 60 cases from 56 patients of any age of both sexes requiring full thickness skin graft and fulfilling the pre-set inclusion and exclusion criteria, were selected for the study. Of whom 30 cases were tagged as Group A (Quilting) while the rest 30 as Group B, which was managed with tie-over dressing. Variable outcomes like epidermal loss, partial dermal loss and full thickness loss were observed in both groups. In quilting group excellent result was achieved in 22(73.3%), good 2(6.7%), satisfactory 3(10%) and poor in 3(10%) patient. In tie-over group, it was 19(63.3%), 3(10%), 3(10%) and 5(16.7%) accordingly. So, the good take were 27(90%) in quilting group and 25(83.3%) in tie-over group. Haematoma recorded in 3 (10%) of quilted group and 5 (16.7%) of tie-over group. No infection occurred in any cases of any group. There were no other recorded complications or adverse outcomes directly related to the technique for securing the grafts in either group. The results demonstrate no significant difference in graft-take comparing grafts secured with a tie-over dressing or by quilting. DOI: http://dx.doi.org/10.3329/bdjps.v3i2.18249 Bangladesh Journal of Plastic Surgery July 2012, 3(2): 38-44

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Dermatologic Surgical Pearls Part 4: Tips for Full-Thickness Skin Grafts

Journal of Cutaneous Medicine and Surgery ◽

10.2310/7750.2014.14097 ◽

2015 ◽

Vol 19 (3) ◽

pp. 294-296

Author(s):

Melissa Nantel-Battista ◽

Christian Murray

Keyword(s):

Skin Graft ◽

Dermatologic Surgery ◽

Skin Grafts ◽

Full Thickness ◽

Office Setting ◽

Full Thickness Skin ◽

Thickness Skin

Dermatologic surgery procedures can sometimes be time consuming in an office setting. We present a series of tips for dermatologists and dermatologic surgeons that will enhance the efficiency of skin graft procedures. Les interventions en chirurgie dermatologique peuvent parfois prendre beaucoup de temps en cabinet. Seront présentés aux dermatologues et aux chirurgiens dermatologues une serie de conseils qui auront pour effet d'accroître l'efficacité des operations touchant les greffes de peau.

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The Effect of Adipose-Derived Stem Cells on Full-Thickness Skin Grafts

BioMed Research International ◽

10.1155/2016/1464725 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 3

Author(s):

Juan Wang ◽

Haojie Hao ◽

Hong Huang ◽

Deyun Chen ◽

Yan Han ◽

...

Keyword(s):

Graft Survival ◽

Skin Graft ◽

Macrophage Polarization ◽

Collagen Type I ◽

Type I ◽

Skin Grafts ◽

Full Thickness ◽

Full Thickness Skin ◽

Thickness Skin ◽

Anti Inflammatory

Background. The purpose of this study was to evaluate the effects of ASCs on full-thickness skin grafts. Specifically, we investigated the anti-inflammatory effects of ASCs that are mediated via regulation of the phenotypes of activated macrophages. Methods. ASCs were isolated, cultured, and injected under full-thickness skin grafts in 15 rats (ASC group). An additional 15 rats served as controls (PBS group). Skin graft survival assessment and vascularization detection were assessed with H&E staining and laser Doppler blood flowmetry (LDF). The effects of ASCs on angiogenesis, anti-inflammation, collagen accumulation-promoting, and antiscarring were assessed. Results. We found that the skin graft survival rate was significantly increased in the ASC group. The neovascularization, collagen deposition, collagen type I to type III ratio, and levels of VEGF and TGF-β3 in the ASC group were markedly higher than those in the PBS group at day 14. Additionally, in the ASC group, the levels of iNOS, IL-1β, and TNF-α were remarkably decreased, whereas the levels of IL-10 and Arg-1 were substantially increased. Conclusions. Our results confirm that ASCs transplantation can effectively improve full-thickness skin graft survival. Additionally, the anti-inflammatory role of ASCs may indirectly contribute to skin graft survival via its effect on macrophage polarization.

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Outcomes of split vs full-thickness skin grafts in scalp reconstruction in outpatient local anaesthetic theatre

Scars Burns & Healing ◽

10.1177/20595131211056542 ◽

2021 ◽

Vol 7 ◽

pp. 205951312110565

Author(s):

Luxi Sun ◽

Animesh JK Patel

Keyword(s):

Skin Graft ◽

Local Anaesthetic ◽

Skin Grafting ◽

Skin Grafts ◽

Full Thickness ◽

Full Thickness Skin ◽

Graft Take ◽

Thickness Skin ◽

Graft Reconstruction ◽

Scalp Skin

Background Surgical excision remains the cornerstone of simultaneous diagnosis and treatment of suspicious skin lesions, and the scalp is a high-risk area for skin cancers due to increased cumulative lifetime ultraviolet (UV) exposure. Due to the inelasticity of scalp skin, most excisions with predetermined margins require reconstruction with skin grafting. Methods A retrospective single-centre cohort study was performed of all patients undergoing outpatient local anaesthetic scalp skin excision and skin graft reconstruction in the Plastic Surgery Department at Addenbrookes Hospital over a 20-month period between 1 April 2017 and 1 January 2019. In total, 204 graft cases were collected. Graft reconstruction techniques included both full-thickness and split-thickness skin grafts. Statistical analysis using Z tests were used to determine which skin grafting technique achieved better graft take. Results Split-thickness skin grafts had a statistically significant ( P = 0.01) increased average take (90%) compared to full-thickness skin grafts (72%). Using a foam tie-over dressing on the scalp led to a statistically significant ( P = 0.000036) increase in skin graft take, from 38% to 79%. Conclusion In skin graft reconstruction of scalp defects after skin cancer excision surgery, split skin grafts secured with foam tie-over dressings are associated with superior outcomes compared to full-thickness skin grafts or grafts secured with sutures only.

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Utilizing Full-Thickness Skin Grafts for Vaginal Reconstruction

Clinics in Plastic Surgery ◽

10.1016/s0094-1298(20)31421-8 ◽

1988 ◽

Vol 15 (3) ◽

pp. 443-448 ◽

Cited By ~ 4

Author(s):

Richard C. Sadove ◽

Charles E. Horton

Keyword(s):

Skin Grafts ◽

Full Thickness ◽

Vaginal Reconstruction ◽

Full Thickness Skin ◽

Thickness Skin

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Early wound repair versus later scar repair in children with treadmill hand friction burns

Journal of Burn Care & Research ◽

10.1093/jbcr/irab083 ◽

2021 ◽

Author(s):

Rong Zhou ◽

Lin Qiu ◽

Jun Xiao ◽

Xiaobo Mao ◽

Xingang Yuan

Keyword(s):

Skin Graft ◽

Wound Repair ◽

Full Thickness ◽

Full Thickness Skin Graft ◽

Vacuum Sealing ◽

Full Thickness Skin ◽

Thickness Skin ◽

Vacuum Sealing Drainage ◽

Repair Group ◽

Hand Friction

Abstract The incidence of pediatric treadmill hand friction burns has been increasing every year. The injuries are deeper than thermal hand burns, the optimal treatment remains unclear. This was a retrospective study of children who received surgery for treadmill hand friction burns from January 1, 2015, to December 31, 2019, in a single burn center. A total of 22 children were surveyed. The patients were naturally divided into two groups: the wound repair group (13 patients), which was admitted early to the hospital after injury and received debridement and vacuum sealing drainage initially, and a full-thickness skin graft later; and the scar repair group (9 patients), in which a scar contracture developed as a result of wound healing and received scar release and skin grafting later. The Modified Michigan Hand Questionnaire score in the wound repair group was 116.31 ± 10.55, and the corresponding score in the scar repair group was 117.56 ± 8.85 (P>0.05), no statistically significant difference. The Vancouver Scar Scale score in the wound repair group was 4.15 ± 1.21, and the corresponding score in the scar repair group was 7.22 ± 1.09 (P<0.05). Parents were satisfied with the postoperative appearance and function of the hand. None in the two groups required secondary surgery. If the burns are deep second degree, third degree, or infected, early debridement, vacuum sealing drainage initially, and a full-thickness skin graft can obviously relieve pediatric pain, shorten the course of the disease, and restore the function of the hand as soon as possible.

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