scholarly journals Molecular cloning and characterization of NAC genes from four foxtail millet genotypes

2019 ◽  
pp. 44-49
Author(s):  
Sintho Wahyuning Ardie ◽  
Nurul Khumaida ◽  
Tetsuo Takano ◽  
Nike Karjunita ◽  
Muhammad Habib Widyawan
Keyword(s):  
Stress Responses ◽  
Genomic Dna ◽  
Foxtail Millet ◽  
High Similarity ◽  
Tolerance Mechanism ◽  
Specific Primer ◽  
Transcription Factor Gene ◽  
Conserved Domain ◽  
Transcription Factor Gene Family

Transcription factor gene family of NAC (NAM, ATAF, CUC) is tightly involved in plant development and in the response to stresses. In this study, we reported the isolation and the characterization of NAC gene homolog from four foxtail millet genotypes. Band with approximately 1300 bp size was successfully amplified from the genomic DNA of four foxtail millet genotypes (ICERI-4, ICERI-5, ICERI-6 and ICERI-10) using gene specific primer. The fragment was designated as SiNAC065 after showing high similarity with NAC gene homologs in the GenBank. Sequence analysis results showed that the SiNAC065 genes isolated from the four genotypes were 1265 bp in length with one intron and two exons. The two exons encode 325 amino acids with the conserved domain located between amino acid 19-325. The SiNAC065 protein identified in this study have 8 conserved motives in the conserved region which categorized them as SNAC (stress responsive NACs) orthologs that are involved in the abiotic stress responses. Different features of SiNAC065 isolated from the tolerant- and the sensitive-genotypes should provide information of the gene’s role in salinity tolerance mechanism of foxtail millet.

scholarly journals Multifarious Roles of GRAS Transcription Factors in Plants

2021 ◽  
Author(s):  
Priya Kumari ◽  
Mrinalini Kakkar ◽  
Vijay Gahlaut ◽  
Vandana Jaiswal ◽  
Sanjay Kumar
Keyword(s):  
Stress Responses ◽  
Crop Improvement ◽  
Structural Features ◽  
Biological Processes ◽  
Interacting Proteins ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Conserved Domain ◽  
Improvement Programs ◽  
Transcription Factor Gene Family

The GAI‐RGA ‐ and ‐SCR (GRAS) proteins belong to the plant-specific transcription factor gene family and involved in several developmental processes, phytohormone and phytochrome signaling, symbiosis, stress responses etc. GRAS proteins have a conserved GRAS domain at C-terminal and hypervariable N-terminal. The C-terminal conserved domain directly affects the function of the GRAS proteins. For instance, in Arabidopsis, mutations in this domain in Slender rice 1 (SLR1) and Repressor of GA (RGA) proteins cause significant phenotypic changes. GRAS proteins have been reported in more than 30 plant species and till now it has been divided into 17 subfamilies. This review highlighted GRAS protein's importance during several biological processes in plants, structural features of GRAS proteins, their expansion and diversification in the plants, GRAS-interacting proteins complexes and their role in biological processes. We also summarized available recent research that utilized CRISPR-Cas9 technology to manipulate GRAS genes in a plant for different traits. Further, the exploitation of GRAS genes in crop improvement programs has also been discussed

Analysis of the NAC transcription factor gene family in citrus reveals a novel member involved in multiple abiotic stress responses

2011 ◽  
Vol 7 (6) ◽  
pp. 1123-1134 ◽  
Author(s):  
Tahise M. de Oliveira ◽  
Luciana C. Cidade ◽  
Abelmon S. Gesteira ◽  
Maurício A. Coelho Filho ◽  
Walter S. Soares Filho ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Abiotic Stress ◽  
Gene Family ◽  
Stress Responses ◽  
Nac Transcription Factor ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Transcription Factor Gene Family

scholarly journals Characterization of germline porcine endogenous retroviruses from Large White pig

2004 ◽  
Vol 85 (8) ◽  
pp. 2421-2428 ◽  
Author(s):  
Linda Scobie ◽  
Samantha Taylor ◽  
Nicola A. Logan ◽  
Sharon Meikle ◽  
David Onions ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Endogenous Retroviruses ◽  
High Similarity ◽  
Miniature Swine ◽  
Large White ◽  
Safety Aspects ◽  
Porcine Endogenous Retroviruses ◽  
Large White Pig

Porcine endogenous retroviruses (PERV) are of concern when the microbiological safety aspects of xenotransplantation are considered. Four unique isolates of PERV B have been identified previously from a lambda library constructed from genomic DNA from a Large White pig. This study shows that none of these isolates are replication competent when transfected into permissive human or pig cells in vitro, and the removal of flanking genomic sequences does not confer a human tropic replication competent (HTRC) phenotype on these PERV proviruses. Analysis of the envelope sequences revealed that PERV B demonstrated high similarity to the envelope sequences derived from replication-competent PERV, indicating that lack of replication competence does not appear to be attributable to this region of the provirus. These data complement recent findings that HTRC PERV are recombinants between the PERV A and PERV C subgroups, and that these recombinants are not present in the germline of miniature swine. Together, these results indicate that these individual PERV B proviruses are unlikely to give rise to HTRC PERV.

A characterization of grapevine of GRAS domain transcription factor gene family

2016 ◽  
Vol 16 (4) ◽  
pp. 347-363 ◽  
Author(s):  
Xin Sun ◽  
Zhengqiang Xie ◽  
Cheng Zhang ◽  
Qian Mu ◽  
Weimin Wu ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Gene Family ◽  
Transcription Factor Gene ◽  
Factor Gene ◽  
Transcription Factor Gene Family
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