scholarly journals Identification of anti-inflammatory compound/compounds in hexane fraction of Jatropha curcas root extract

2019 ◽  
pp. 62-68
Author(s):  
Ahmad Razi Othman ◽  
Intan Safinar Ismail ◽  
Norhani Abdullah ◽  
Syahida Ahmad
Keyword(s):  
Fatty Acid ◽  
Jatropha Curcas ◽  
Octadecadienoic Acid ◽  
Inflammatory Activity ◽  
Cytotoxic Activities ◽  
Preparative Hplc ◽  
Root Extract ◽  
Raw 264.7 ◽  
Carbon Fatty Acid ◽  
Anti Inflammatory

Jatropha curcas is a medicinal plant with many therapeutic properties such as anti-inflammatory, anti-malaria, anti-cancer and antioxidant. The root extract has been shown to possess high anti-inflammatory activity. Previously, the compounds responsible for this activity have not been fully elucidated. Two fractions (Fraction 1 and Fraction 2) obtained from a preparative HPLC of the root extract showed significant anti-inflammatory and cytotoxic activities in RAW 264.7 murine macrophage cells with Fraction 1 giving higher nitric oxide (NO) inhibition compared to Fraction 2 and L-NAME. Further purification steps involving column chromatography, thin layer chromatography and analytical HPLC of Fraction 1 produced two fractions labeled as Fraction A and Fraction B. Both fractions showed anti-inflammatory activity without cytotoxic activity in RAW 264.7 cells. Liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis showed that Fraction A contained a group of 18 carbon fatty acid compounds consisting of 2 oxooctadecanoic acids; 15, 16 dihydroxy 9Z, 12Z octadecadienoic acid; octadecadienoic isomer and 15,16 dihydroxy 9Z, 12Z octadecadienoic acid, 15S, 16S. The 18-carbon fatty acid structure was confirmed by nuclear magnetic resonance (NMR) spectral data. The IC50 value of compounds in Fraction A for anti-inflammatory activity in RAW 264.7 cell line was 434.8±0.75 µg/mL. From the analysis, it can be concluded that Fraction A can be classified under 18 carbon long chain fatty acid group based on LC MS/MS and NMR analysis. This active compound shows an inhibition towards NO activity.

Synthesis and anti-inflammatory activity of novel steroidal chalcones with 3β-pregnenolone ester derivatives in RAW 264.7 cells in vitro

Steroids ◽  
2021 ◽  
pp. 108830
Author(s):  
Xiaorui Cai ◽  
Fei Sha ◽  
Chuanyi Zhao ◽  
Zhiwei Zheng ◽  
Shulin Zhao ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Anti Inflammatory

scholarly journals Anti-inflammatory activity and modulate oxidative stress of Bucida buceras in lipopolysaccharide-stimulated RAW 264.7 macrophages and Carrageenan-induced acute paw edema in rats

2017 ◽  
Vol 11 (12) ◽  
pp. 239-252 ◽  
Author(s):  
B. Iloki Assanga Simon ◽  
M. Lewis Luján Lidianys ◽  
A. Gil-Salido Armida ◽  
L. Lara Espinoza Claudia ◽  
Fernandez Angulo Daniela ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inflammatory Activity ◽  
Raw 264.7 ◽  
Paw Edema ◽  
Raw 264.7 Macrophages ◽  
Anti Inflammatory

RETRACTED: Anti-inflammatory Activity of 4-Arylcoumarins from EndophyticStreptomyces aureofaciensCMUAc130 in Murine Macrophage RAW 264.7 Cells

2007 ◽  
Vol 36 (2) ◽  
pp. 203-211 ◽  
Author(s):  
Thongchai Taechowisan ◽  
Pittaya Tuntiwachwuttikul ◽  
Chunhua Lu ◽  
Yuemao Shen ◽  
Saisamorn Lumyong ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Anti Inflammatory

scholarly journals IN VITRO ANTI-INFLAMMATORY ACTIVITY OF THE COMPONENTS OF AMOMUM TSAO-KO IN MURINE MACROPHAGE RAW 264.7 CELLS

2018 ◽  
Vol 15 (2) ◽  
pp. 26-34
Author(s):  
Chun Whan Choi ◽  
Ju Young Shin ◽  
Changon Seo ◽  
Seong Su Hong ◽  
Eun-Kyung Ahn ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Murine Macrophage ◽  
Anti Inflammatory

scholarly journals Anti-Inflammatory Activity of Geldanamycin and Its Derivatives in LPS-Induced RAW 264.7 Cells

2019 ◽  
Vol 09 (04) ◽  
pp. 398-421 ◽  
Author(s):  
Thongchai Taechowisan ◽  
Winyou Puckdee ◽  
Watcharee Waratchareeyakul ◽  
Waya S. Phutdhawong
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Anti Inflammatory

Anti-inflammatory Activity of Beta vulgaris Extract Using Biorenovation in LPS-stimulated RAW 264.7 Cells

KSBB Journal ◽  
2021 ◽  
Vol 36 (2) ◽  
pp. 123-129
Author(s):  
Hyehyun Hong ◽  
Taejin Park ◽  
Min-Sung Kang ◽  
Seung-Young Kim
Keyword(s):  
Beta Vulgaris ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
Raw 264.7 ◽  
Anti Inflammatory

scholarly journals Cordyceps militaris Fungus Extracts-Mediated Nanoemulsion for Improvement Antioxidant, Antimicrobial, and Anti-Inflammatory Activities

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5733
Author(s):  
Esrat Jahan Rupa ◽  
Jin Feng Li ◽  
Muhammad Huzaifa Arif ◽  
Han Yaxi ◽  
Aditi Mitra Puja ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Tween 80 ◽  
In Vitro Cytotoxicity ◽  
Cordyceps Militaris ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Anti Inflammatory

This study aimed to produce and optimize a Cordyceps militaris-based oil-in-water (O/W) nanoemulsion (NE) encapsulated in sea buckthorn oil (SBT) using an ultrasonication process. Herein, a nonionic surfactant (Tween 80) and chitosan cosurfactant were used as emulsifying agents. The Cordyceps nanoemulsion (COR-NE) was characterized using Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), and field-emission transmission electron microscope (FE-TEM). The DLS analyses revealed that the NE droplets were 87.0 ± 2.1 nm in diameter, with a PDI value of 0.089 ± 0.023, and zeta potential of −26.20 ± 2. The small size, low PDI, and stable zeta potential highlighted the excellent stability of the NE. The NE was tested for stability under different temperature (4 °C, 25 °C, and 60 °C) and storage conditions for 3 months where 4 °C did not affect the stability. Finally, in vitro cytotoxicity and anti-inflammatory activity were assessed. The results suggested that the NE was not toxic to RAW 264.7 or HaCaT (human keratinocyte) cell lines at up to 100 µL/mL. Anti-inflammatory activity in liposaccharides (LPS)-induced RAW 264.7 cells was evident at 50 µg/mL and showed inhibition of NO production and downregulation of pro-inflammatory gene expression. Further, the NE exhibited good antioxidant (2.96 ± 0.10 mg/mL) activity and inhibited E. coli and S. aureus bacterial growth. Overall, the COR-NE had greater efficacy than the free extract and added significant value for future biomedical and cosmetics applications.

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