scholarly journals Leishmania infantum lipophosphoglycan, a novel carbohydrate-based antigen for the immunodiagnosis of canine visceral leishmaniasis

2019 ◽  
Author(s):  
Rodrigo Soares ◽  
Ricardo Portela ◽  
Gabriela Porfírio-Passos ◽  
Thiago Barral ◽  
Rafaela Gonçalves ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Canine Visceral Leishmaniasis

scholarly journals Performance of an indigenous β-mercaptoethanol-modified antigen in comparison with a commercial reference in direct agglutination test for detection of canine visceral leishmaniasis

2014 ◽  
Vol 63 (1) ◽  
pp. 106-110
Author(s):  
Saul José Semião-Santos ◽  
Laura Barral Veloso ◽  
Paulo Paes de Andrade ◽  
Marcia Almeida de Melo ◽  
Luis Miguel Lourenço Martins ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Leishmania Donovani ◽  
Agglutination Test ◽  
Canine Visceral Leishmaniasis ◽  
Direct Agglutination Test ◽  
Serum Samples

We compared the performance of a locally produced β-mercaptoethanol-modified promastigote antigen (β-ME-Ag) of an indigenous Leishmania infantum strain against that of a trypsinized Leishmania donovani reference (REF-Ag) in the direct agglutination test (DAT) for detection of canine visceral leishmaniasis (CVL). One hundred and fifty-one serum samples collected from dogs belonging to four groups with different conditions were included. At a DAT titre of 1 : 320, statistically determined as optimal cut-off value for β-ME-Ag, and 1 : 160 for REF-Ag, a sensitivity and a specificity of 100 % were estimated for β-ME-Ag in comparison with 96.6 % and 100 %, respectively, for REF-Ag. Overall, levels of agglutination titres recorded for the two antigens were highly concordant (Cohen’s κ = 0.879) in both the CVL and non-CVL groups. Based on current results, and ease experienced in processing the antigen and reading the test outcome, we recommend incorporation of β-ME-Ag in DAT for confirmation or exclusion of suspected CVL in dogs.

scholarly journals High Performance of ELISA test using recombinant rLiNTPDase2 from Leishmania infantum: a Phase II diagnosis of Canine Visceral Leishmaniasis

Acta Tropica ◽  
2020 ◽  
Vol 209 ◽  
pp. 105535
Author(s):  
Anna Cláudia Alves de Souza ◽  
Raissa Barbosa de Castro ◽  
Yaro Luciolo dos Santos ◽  
Nancy da Rocha Torres Pavione ◽  
Joice de Melo Agripino ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Phase Ii ◽  
Leishmania Infantum ◽  
High Performance ◽  
Elisa Test ◽  
Canine Visceral Leishmaniasis

scholarly journals A multicentric evaluation of the recombinant Leishmania infantum antigen-based immunochromatographic assay for the serodiagnosis of canine visceral leishmaniasis

2014 ◽  
Vol 7 (1) ◽  
pp. 136 ◽  
Author(s):  
Deborah Bittencourt Fraga ◽  
Edimilson da Silva ◽  
Luciano Pacheco ◽  
Lairton Borja ◽  
Isaac de Oliveira ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Immunochromatographic Assay ◽  
Canine Visceral Leishmaniasis

Canine visceral leishmaniasis caused by Leishmania infantum in Senegal: risk of emergence in humans?

2010 ◽  
Vol 12 (14-15) ◽  
pp. 1219-1225 ◽  
Author(s):  
B. Faye ◽  
A.L. Bañuls ◽  
B. Bucheton ◽  
M.M. Dione ◽  
O. Bassanganam ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Canine Visceral Leishmaniasis

Evaluation of Leishmania infantum pyridoxal kinase protein for the diagnosis of human and canine visceral leishmaniasis

2020 ◽  
Vol 220 ◽  
pp. 11-20 ◽  
Author(s):  
João A. Oliveira-da-Silva ◽  
Amanda S. Machado ◽  
Fernanda F. Ramos ◽  
Grasiele S.V. Tavares ◽  
Daniela P. Lage ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Canine Visceral Leishmaniasis ◽  
Pyridoxal Kinase

Evaluation of three recombinant Leishmania infantum antigens in human and canine visceral leishmaniasis diagnosis

Acta Tropica ◽  
2014 ◽  
Vol 137 ◽  
pp. 25-30 ◽  
Author(s):  
Aliani Moura Fonseca ◽  
Angélica Rosa Faria ◽  
Fernandes Tenório Gomes Rodrigues ◽  
Ronaldo Alves Pinto Nagem ◽  
Rubens Daniel Miserani Magalhães ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Leishmania Infantum ◽  
Canine Visceral Leishmaniasis

scholarly journals Molecular diagnosis of canine visceral leishmaniasis: A comparative study of three methods using skin and spleen from dogs with natural Leishmania infantum infection

2013 ◽  
Vol 197 (3-4) ◽  
pp. 498-503 ◽  
Author(s):  
Levi Eduardo Soares Reis ◽  
Wendel Coura-Vital ◽  
Bruno Mendes Roatt ◽  
Leoneide Érica Maduro Bouillet ◽  
Henrique Gama Ker ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Comparative Study ◽  
Molecular Diagnosis ◽  
Leishmania Infantum ◽  
Canine Visceral Leishmaniasis

scholarly journals The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive Dogs

2016 ◽  
Vol 10 (1) ◽  
pp. e0004333 ◽  
Author(s):  
Deborah Bittencourt Mothé Fraga ◽  
Luciano Vasconcellos Pacheco ◽  
Lairton Souza Borja ◽  
Pétala Gardênia da Silva Estrela Tuy ◽  
Leila Andrade Bastos ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
False Positive ◽  
Leishmania Infantum ◽  
Rapid Test ◽  
Canine Visceral Leishmaniasis
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