scholarly journals Uso de la imagen radiológica y serología por Western Blot para el diagnóstico de la neurocisticercosis en un hospital del norte del Perú

2021 ◽  
Vol 14 (3) ◽  
pp. 311-315
Author(s):  
Johnny Leandro Saavedra-Camacho ◽  
Mayra Massely Coico-Vega ◽  
Virgilio E. Failoc-Rojas ◽  
Benigno Ballón-Manrique ◽  
Heber Silva-Díaz
Keyword(s):  
Western Blot ◽  
Taenia Solium

Introducción: La neurocisticercosis (NCC) es una zoonosis parasitaria del sistema nervioso central causada por el céstodo Taenia solium, y que afecta a países en desarrollo y con escaso saneamiento básico. Objetivo: Describir el uso y la concordancia de la imagen radiológica por tomografía (TAC) o resonancia magnética (RM) y serología por western Blot (WB) en el diagnóstico de la NCC en un hospital del norte del Perú. Material y Métodos: Estudio observacional retrospectivo. La historia clínica fue la unidad de análisis. Los casos se buscaron en la oficina de Epidemiología mediante el CIE-10- B69 y registro del Laboratorio de Parasitología, Metaxénicas y Zoonosis del mismo hospital, en el periodo del año 2015 al 2017. Resultados: Se estudiaron 67 historias clínicas, que cumplían con los criterios diagnósticos absolutos de NCC. Los pacientes fueron varones en el 55,2 % y tuvieron una media de edad de 40,2 (±17,8) años. El 35,9% tuvieron un resultado positivo por WB (19/39), las lesiones quísticas con escólex fueron observada en el 25,4% de las TAC y en el 29,6 de las RM. La concordancia observada entre la prueba serológica con TAC y RM fue escasa, siendo (Kappa=0,073, IC95%: 0,053 – 1,084) y (Kappa=0,112, IC95%: 0,092 – 1,092) y un porcentaje de acuerdo de 42,0% y 45,7% respectivamente. Conclusiones: Se observó uso diferenciado y escasa concordancia entre la prueba serológica por WB e imagen radiológica en pacientes con diagnóstico de neurocisticercosis en la población estudiada.

Toxocariasis/cysticercosis seroprevalence in a long-term rural settlement, São Paulo, Brazil

Parasitology ◽  
2009 ◽  
Vol 136 (6) ◽  
pp. 681-689 ◽  
Author(s):  
L. E. PRESTES-CARNEIRO ◽  
D. H. P. SOUZA ◽  
G. C. MORENO ◽  
C. TROIANI ◽  
V. SANTARÉM ◽  
...  
Keyword(s):  
Western Blot ◽  
Taenia Solium ◽  
Sao Paulo ◽  
Rural Settlement ◽  
São Paulo ◽  
Educational Levels ◽  
Second Stage ◽  
Paulo State ◽  
Ocular Manifestations

SUMMARYSeroprevalence of Toxocara and Taenia solium and risk factors for infection with these parasites were explored in a long-term rural settlement in São Paulo state, Brazil. An ELISA for the detection of anti-Toxocara IgG and IgE and anti-T. solium cysticerci was standardized using Toxocara excretory-secretory antigens (TES) obtained from the cultured second-stage larvae of T. canis and by vesicular fluid antigen from Taenia crassiceps cysticerci (VF). For cysticercosis, the reactive ELISA samples were assayed by Western blot using 18 kDa and 14 kDa proteins purified from VF. Out of 182 subjects, 25 (13·7%) presented anti-Toxocara IgG and a positive correlation between total IgE and the reactive index of specific anti-TES IgE (P=0·0265) was found amongst the subjects found seropositive for anti-Toxocara IgG. In these individuals 38·0% showed ocular manifestations. The frequency of anti-T. solium cysticerci confirmed by Western blot was 0·6%. Seropositivity for Toxocara was correlated with low educational levels and the owning of dogs. Embryonated eggs of Toxocara spp. were found in 43·3% of the analysed areas.

scholarly journals Prevalencia de cisticercosis en porcinos de la provincia de Tambopata, Perú

2021 ◽  
Vol 1 (42) ◽  
pp. 17-18
Author(s):  
Robinson Rojas Rivera
Keyword(s):  
Western Blot ◽  
Taenia Solium ◽  
Vena Cava

La cisticercosis es una de las principales enfermedades zoonóticas parasitarias que es causada por el establecimiento de la forma larvaria de Taenia solium. Esta enfermedad se desarrolla principalmente en cerdos que son criados en granjas sin tecnificación, donde el uso de la tecnología y las condiciones sanitarias son mínimas. Este tipo de crianza es muy usual, por lo que representa un riesgo de la salud pública. En ese sentido, se determinó la prevalencia de cisticercosis en porcinos de la provincia de Tambopata, donde fue evaluado un total de 98 porcinos. Se tomaron aproximadamente 5 ml de sangre de la vena cava en animales mayores de 6 meses y hembras que no estuviesen preñadas; posteriormente, se obtuvo el plasma para ser procesado mediante la prueba de enzyme-linked inmunoelectrotransfer bloot assay (EITB) o Western Blot. Se determinó que el 17 % de los cerdos evaluados dio positivo para cisticercosis; con respecto al sexo, se obtuvo una seroprevalencia de 5,21 % ± 0,82 % para machos y 11,45 % ± 1,93 % para hembras. Finalmente, se determinó una seroprevalencia de 10,41 % ± 1,75 % para animales jóvenes de 6 a 11 meses y 6,25 % ± 1,01 % para animales adultos de 12 meses a más. Estos resultados reflejan la importancia de la vigilancia y control de las enfermedades parasitarias en los animales de producción ya que pudo corroborarse que la cisticercosis porcina constituye un serio problema de salud pública

Immunocharacterization ofTaenia soliumoncosphere and metacestode antigens

1996 ◽  
Vol 70 (4) ◽  
pp. 271-280 ◽  
Author(s):  
C. Garcia-Allan ◽  
N. Martínez ◽  
A. Flisser ◽  
A. Aluja ◽  
J.C. Allan ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Western Blot ◽  
Experimental Infection ◽  
Blot Analysis ◽  
Post Infection ◽  
Western Blot Analysis ◽  
Taenia Solium ◽  
Specific Antibodies ◽  
Specific Antigens ◽  
Related Increase

AbstractA partial immunocharacterization of oncosphere and metacestode antigens ofTaenia soliumwas carried out and compared to antigens from other taeniid species. The results indicated thatT. soliummetacestode antigen contained epitopes cross reactive with rabbit anti-sera to adult and oncospheral stages of the parasite. Oncospheres, however, consisted largely of stage specific antigens. Western blot analysis indicated thatT. soliumandT. pisiformisshared several oncospheral antigens; however, this was not the case withT. soliumandT. hydatigena. Western blot analysis showed a time-related increase in the number of molecules recognized by antibodies toT. soliumoncosphere and metacestode antigens in pigs experimentally infected withT. soliumeggs. Oncosphere specific antibodies were detected in pig sera one month after experimental infection whereas antibodies to cystic stage antigens were not present until the 3rd to 5th month post infection. Sera from neurocysticercotic patients as well as naturally infected cysticercotic pigs recognized high molecular weight antigens in the oncospheres.

scholarly journals Cysticercosis in epileptic patients of Mulungu do Morro Northeastern Brazil

2000 ◽  
Vol 58 (3A) ◽  
pp. 621-624 ◽  
Author(s):  
IRENIO GOMES ◽  
MARIELZA VEIGA ◽  
DOLORES CORREA ◽  
ANTONIO MEZA-LUCAS ◽  
OLGA MATA ◽  
...  
Keyword(s):  
Western Blot ◽  
Epileptic Seizure ◽  
Arid Region ◽  
Taenia Solium ◽  
Northeastern Brazil ◽  
Ambulatory Clinic ◽  
Capture Elisa ◽  
Epileptic Patients ◽  
Specific Fraction

With the aim to study the magnitude of infection by the metacestode of Taenia solium in a population of epileptic patients in the arid region of Bahia, Northeastern Brazil, we examined 200 consecutive cases who attended an ambulatory clinic in the disctrict of Mulungu do Morro. Sixty-six of the patients had a diagnosis of epilepsy. From them 10 (15.2%) presented antibodies against a specific fraction of antigens in Western blot, and 4 (6.0%) had circulating parasite products, as tested by capture ELISA. Only 1 case was positive for antibodies and antigens. We found that the frequency of seropositivity was related to the time without epileptic seizure. We conclude that cysticercosis is endemic in the region of Mulungu do Morro and that it is related to a benign form of epilepsy.

Immunolocalization ofTaenia soliumgap junction innexins

Parasitology ◽  
2008 ◽  
Vol 135 (9) ◽  
pp. 1125-1131 ◽  
Author(s):  
R. ZURABIAN ◽  
A. LANDA ◽  
L. ROBERT ◽  
K. WILLMS
Keyword(s):  
Amino Acid ◽  
Western Blot ◽  
Small Molecules ◽  
Synthetic Peptide ◽  
Polyclonal Antibodies ◽  
Taenia Solium ◽  
Peptide Sequence ◽  
Rabbit Igg ◽  
Membrane Peptide ◽  
Cytoplasmic Glycogen

SUMMARYIn previous studies, ultrastructural observations revealed a large number of gap junctions (GJs) in the neck and immature proglottid tissues ofTaenia soliumtapeworms. In these helminths, cytoplasmic glycogen sacs are connected by numerous discrete GJs to other cells throughout the maturing strobilar tissue. Discontinuous sucrose gradients were used to purify membrane fractions containing GJs, which were identified by ultrastructural analysis. A trans-membrane peptide sequence from a highly conserved innexin region was used to construct a 20-amino acid synthetic peptide and used to raise polyclonal antibodies in rabbits that recognized both a 55 and a 67 kDa protein in a Western blot of the GJ-enriched pellet. Immunohistochemistry of larval and adult worm sections incubated with antiserum to the synthetic peptide and a secondary anti-rabbit IgG bound to fluorescein, revealed strong binding to the tegumentary surface of the worm, as well as patchy fluorescent areas in the parenchyma. The results indicate that both the tegument of cysticerci and adultT. soliumcontain innexin-rich membranes, which may function as a tegumentary transport system for small molecules.

A western blot and ELISA assay for the diagnosis of Taenia solium infection in pigs

1994 ◽  
Vol 53 (3-4) ◽  
pp. 209-217 ◽  
Author(s):  
K.M.L. Pathak ◽  
J.C. Allan ◽  
K. Ersfeld ◽  
P.S. Craig
Keyword(s):  
Western Blot ◽  
Taenia Solium ◽  
Elisa Assay

scholarly journals The Hamster Model for Identification of Specific Antigens ofTaenia soliumTapeworms

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Alicia Ochoa-Sánchez ◽  
Lucía Jiménez ◽  
Abraham Landa
Keyword(s):  
Western Blot ◽  
Adult Stage ◽  
Taenia Solium ◽  
Pork Meat ◽  
Western Blot Assay ◽  
Hamster Model ◽  
Porcine Cysticercosis ◽  
Crude Extracts ◽  
Specific Antigens

Humans acquire taeniasis by ingesting pork meat infected withTaenia soliumcysticerci, which are the only definitive hosts of the adult stage (tapeworm) and responsible for transmitting the human and porcine cysticercosis. Hence, detection of human tapeworm carriers is a key element in the development of viable strategies to control the disease. This paper presents the identification of specific antigens using sera from hamsters infected withT. soliumtapeworms analyzed by western blot assay with crude extracts (CEs) and excretion-secretion antigens (E/S Ag) obtained fromT. soliumcysticerci and tapeworms and extracts from other helminthes as controls. The hamster sera infected withT. soliumtapeworms recognized specific bands of 72, 48, 36, and 24 kDa, in percentages of 81, 81, 90, and 88%, respectively, using theT. soliumtapeworms E/S Ag. The antigens recognized by these hamster sera could be candidates to improve diagnosis of humanT. soliumtaeniasis.

Vorkommen von Antikörpern gegen das bovine Immunschwächevirus bei Rindern in Bayern

2003 ◽  
Vol 31 (05) ◽  
pp. 248-253
Author(s):  
Maren Bartels ◽  
Katrin Hartmann ◽  
L. Scobie ◽  
O. Jarrett ◽  
W. Klee
Keyword(s):  
Western Blot

ZusammenfassungIm Rahmen einer epidemiologischen Untersuchung zur Infektion mit dem bovinen Immunschwächevirus (BIV) bei Rindern in Oberbayern erfolgten zwei Studien, in denen Serum mittels indirektem ELISA auf BIV-Antikörper untersucht wurde. Die ELISA-Ergebnisse der BIV-positiven Tiere der Studie I wurden mittels Western Blot bestätigt. In Studie I wurde Blut von 173 ungezielt ausgewählten Rinderpatienten der II. Medizinischen Tierklinik der Ludwig-Maximilians-Universität München untersucht. Von diesen waren acht Tiere BIV-infiziert. Das entspricht einer Prävalenz von 4,6%. Alle positiven Tiere waren über zwei Monate alt. In Studie II wurden 550 Kühe aus 11 oberbayerischen landwirtschaftlichen Betrieben untersucht. Hiervon waren 11 Tiere BIVAntikör-perpositiv. Dies entspricht einer Prävalenz von 2,0%. Die positiven Tiere stammten aus fünf Betrieben mit Boxenlaufstallhaltung. Kein Tier aus Betrieben mit Anbindehaltung war positiv. In Studie II lag das Durchschnittsalter der Kühe aus den Betrieben ohne BIV-infizierte Tiere signifikant höher als in den Betrieben mit BIV-infizierten Tieren. Die Prävalenz von BIV-Antikörpern war zwar unter den kranken Probanden aus Studie I signifikant höher als bei den klinisch unauffälligen Rindern der Studie II, die pathogene Bedeutung des BIV erscheint jedoch fraglich.

Isolation and Structural Charaderization of a Potent Inhibitor of Coagulation Factor Xa from the Leech Haementeria ghilianii

1989 ◽  
Vol 61 (03) ◽  
pp. 437-441 ◽  
Author(s):  
Cindra Condra ◽  
Elka Nutt ◽  
Christopher J Petroski ◽  
Ellen Simpson ◽  
P A Friedman ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Salivary Gland ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Western Blot ◽  
Potent Inhibitor ◽  
Factor Xa ◽  
Coagulation Factor ◽  
Sds Page ◽  
Bovine Factor

SummaryThe present work reports the discovery and charactenzation of an anticoagulant protein in the salivary gland of the giant bloodsucking leech, H. ghilianii, which is a specific and potent inhibitor of coagulation factor Xa. The inhibitor, purified to homogeneity, displayed subnanomolar inhibition of bovine factor Xa and had a molecular weight of approximately 15,000 as deduced by denaturing SDS-PAGE. The amino acid sequence of the first 43 residues of the H. ghilianii derived inhibitor displayed a striking homology to antistasin, the recently described subnanomolar inhibitor of factor Xa isolated from the Mexican leech, H. officinalis. Antisera prepared to antistasin cross-reacted with the H. ghilianii protein in Western Blot analysis. These data indicate that the giant Amazonian leech, H. ghilianii, and the smaller Mexican leech, H. officinalrs, have similar proteins which disrupt the normal hemostatic clotting mechanisms in their mammalian host’s blood.

Western Blot Analyses of Prekallikrein and its Activation Products in Human Plasma

1991 ◽  
Vol 65 (04) ◽  
pp. 382-388 ◽  
Author(s):  
Dulce Veloso ◽  
Robert W Colman
Keyword(s):  
Complex Formation ◽  
Western Blot ◽  
Human Plasma ◽  
Contact System ◽  
Plasma Activation ◽  
Normal Plasma ◽  
Sds Page ◽  
Activation Products ◽  
Major Antigen ◽  
Formation Of Complexes

SummaryPrekallikrein (PK), a zymogen of the contact system, and its activation products, kallikrein (KAL), KAl-inhibitor complexes and fragments containing KAL epitope(s) have been detected in human plasma by immunoblotting with a monoclonal anti-human plasma PK antibody, MAb 13G1L. Detection of antigen-MAb 13G11 complexes with peroxidase-conjugated anti-IgG showed that the two variants of PK (85- and 88-kDa) are the only major antigen species in normal, non-activated plasma. Upon plasma activation with kaolin, the intensity of the PK bands decreased with formation of complexes of KAL with CL inhibitor (C1 INH) and α2-rrtzcroglobulin (α2M) identical to those formed by the purified proteins. Immunoblots of normal plasma showed good correlation between the PK detected and the amount of plasma assayed. Increasing amounts of KAL incubated with a constant volume of PK-deficient plasma showed increasing amounts of KAL and of KAL-C1 INH and KAL-α2M complexes. Complexes of KALantithrombin III (ATIII) and the ratio of KALα2M/ KAL-CL INH were higher in activated CL INH-deficient plasmas than in activated normal plasmas. Protein resolution by 3-12% gradient SDS-PAGE and epitope detection with [125I]MAb 13G11 showed four KALα2M species and a 45-kDa fragment(s) in both surface-activated normal plasma and complexes formed by purified KAL and α2M. Immunoblots of activated plasma also showed bands at the position of KALCL INH and KALATIII complexes. When α1-antitrypsin Pittsburgh (cα1-AT, Pitts) was added to plasma before activation, KAL-α1-ALPitts was the main complex. The non-activated normal plasma revealed only an overloaded PK band. This is the first report of an antibody that recognizes KAL epitope(s) in KAL-α2M, KALATIII and KALa1-α1Pitts complexes and in the 45-kDa fragment(s). Therefore, MAb 13G11 should be useful for studying the structure of these complexes as well as the mechanism of complex formation. In addition, immunoblotting with MAb 13G11 would allow detection of KAl-inhibitor complexes in patient plasmas as indicators of activation of the contact system.

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