Optimization for in vitro regeneration in four local cultivars of indica rice

The study was conducted at Biotechnology Division of Bangladesh Rice Research Institute (BRRI) to investigate the effects of plant growing medium and plant growth regulator (PGR) for the callus induction and high frequency plantlets regeneration of indica rice. Ten indica rice varieties viz. BR5, BR11, BRRI dhan28, BRRI dhan29, BRRI dhan33, BRRI dhan41, BRRI dhan47, BRRI dhan48, BRRI dhan49 and BRRI dhan50 were cultured on MS, N6 and LS media. The MS medium was found better for callus induction as compared to N6 and LS media. Among the tested varieties BRRI dhan48 induced the highest percent and best quality callus. Interaction effects of BRRI dhan48 to MS medium yielded 71.55% callus induction. The regeneration efficiency of BRRI dhan48 was tested on MS medium supplemented with different combination of NAA plus BAP and NAA plus kinetin. MS medium supplemented with 2.0 mg L-1 NAA and 2.0 mg L-1 Kn was found the best in respect of percent regenerated (76.67%) plantlet as well as for the growth of plantlets in vitro.Bangladesh Rice j. 2016, 20(2): 81-87

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Optimization of in vitro regeneration protocol for a popular Indica rice (Oryza sativa L. cv Swarna)

Annals of Plant Sciences ◽

10.21746/aps.2016.08.002 ◽

2016 ◽

Vol 5 (08) ◽

pp. 1395 ◽

Cited By ~ 1

Author(s):

Vijaya Naresh Juturu ◽

Gopala Krishna Mekala ◽

Mallikarjuna Garladinne ◽

Puli Chandra Obul Reddy ◽

Akila Chandra Sekhar*

Keyword(s):

Callus Induction ◽

Oryza Sativa L ◽

Indica Rice ◽

In Vitro Regeneration ◽

Regeneration System ◽

Embryogenic Calli ◽

Rice Varieties ◽

Regenerated Plants ◽

Rooting Medium

Though regeneration system in rice has been very well established compare to other crop plants, the fact remains that, most of the indica rice varieties are still recalcitrant for regeneration and genetic transformation. Therefore, refinement of tissue culture protocol for generation of embryogenic calli and regeneration of the fertile plants from a single cell should be a pre requisite event for development of transgenic plants. Here, in this study we reported high frequency robust regeneration protocols for a popular Indica cultivar Swarna.Mature seeds were used as initial material as explants. Highest callus induction % was observed in MSCIMP medium containing 2.0 mg-1 2,4, D + 0.5 mg-1 Kn as phytohormonal combinations. In addition, maximum regeneration was observed in 2.0 mg-l KN + 0.5 mg-l NAA. Regenerated plants were shifted to rooting medium followed by polyhouse for hardening. The callus induction and regeneration reported in this study were well suited for transformation agronomical important genes or functional genomics studies.

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In vitro regeneration protocol for indica rice genotypes

Journal of Bioscience and Agriculture Research ◽

10.18801/jbar.160117.163 ◽

2017 ◽

Vol 16 (1) ◽

pp. 1314-1223

Author(s):

K. Miah ◽

B. Hossen ◽

M. S. Haque ◽

M. Z. Tareq ◽

S. N. Begum

Keyword(s):

Indica Rice ◽

In Vitro Regeneration ◽

Rice Genotypes

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Optimization of an in vitro regeneration system for colombian indica rice varieties

Revista Colombiana de Biotecnología ◽

10.15446/rev.colomb.biote.v16n2.38017 ◽

2014 ◽

Vol 16 (2) ◽

pp. 19-29

Author(s):

Iván Darío Barbosa Cepeda ◽

Alejandro Chaparro Giraldo

Keyword(s):

Indica Rice ◽

In Vitro Regeneration ◽

Regeneration System ◽

Rice Varieties

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In Vitro Regeneration of High Yielding Indica Rice (Oryza sativa L.) Varieties

Journal of Environmental Science and Natural Resources ◽

10.3329/jesnr.v5i1.11573 ◽

2012 ◽

Vol 5 (1) ◽

pp. 173-177 ◽

Cited By ~ 1

Author(s):

MJ Alam ◽

M Imran ◽

L Hassan ◽

MH Rubel ◽

M Shamsuddoha

Keyword(s):

Shoot Regeneration ◽

Callus Induction ◽

Culture Media ◽

Oryza Sativa L ◽

Indica Rice ◽

In Vitro Regeneration ◽

Induction Frequency ◽

Establishment Rate ◽

Number Of Shoots

The experiment was conducted to observe the regeneration potential and also to establish a suitable in vitro plantlet regeneration protocol from mature seed derived embryogenic calli of four indica rice varieties viz BRRI dhan28, BRRI dhan29, BRRI dhan47 and Binadhan-7 after partial desiccation treatment. Different concentrations and combinations of growth regulators were used in MS medium to observe the callus induction ability using mature embryo as explants. The percentage of callus induction frequency was highest (86.00) in BRRI dhan47 and the lowest (56.50) in Binadhan-7 Among the culture media the performance of MS +500 mgl-1 L- Proline + 2.0 mgl-12, 4-D + 0.8 mgl-1BAP was better than any other media for callus induction frequency (%), rapid callusing, size of the callus (mm), texture of callus and color of callus. Among the four varieties, shoot regeneration was highest in BRRI dhan29 (84.33%) which required minimum (14.80) days to and the lowest was in Binadhan-7 (39.67%) which required maximum (15.47) days. Among the treatments, the highest (65.75%) shoot regeneration was observed with MS + 6.0 mgl-1Kn +0.5 mgl-1 NAA which required minimum days (13.75) to develop green bud formation and the lowest shoot regeneration (56.50) was observed with MS + 2.0 mgl-1Kn + .05 mgl-1 NAA. BRRI dhan29 produced more number of shoots (4.67) per callus while Binadhan-7 showed minimum number of shoots (2.87) per callus. The highest number of shoot producing roots (3.66) was observed in BRRI dhan29 which showed maximum number of root per plant and the lowest (3.11) in Binadhan-7. Among the three treatments MS + 0.6 mgl-1IBA showed highest percentage 86.67 of root followed by MS+ 0.6 mgl-1IBA 70% and the lowest (66.7%) was in MS+ 0.4 mgl-1IBA. The establishment rate of the plantlet in the pot was the highest (67.67%) in BRRI dhan29 and Binadhan-7 showed lowest establishment rate (51.22%) in pot. DOI: http://dx.doi.org/10.3329/jesnr.v5i1.11573J. Environ. Sci. & Natural Resources, 5(1): 173 - 177, 2012

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