Improvement of Indica rice (Oryza sativa L.) in vitro regeneration efficiency from callus mediated by stress

1998 ◽  
Vol 26 (2) ◽  
pp. 153-160 ◽  
Author(s):  
Yamilet Coll ◽  
Merardo Pujol ◽  
Damaris Castillo ◽  
Annerys González ◽  
Julio Alfonso ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Regeneration Efficiency

Investigating the In vitro Regeneration Potentiality of Three High Yielding Indica Rice (Oryza sativa L.) Varieties

2015 ◽  
Vol 9 (3) ◽  
pp. 1-13
Author(s):  
Mohammd Hossain ◽  
Nazneen Akter ◽  
Upama Mondal ◽  
Ratan Dey ◽  
Lutful Hassan
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration

scholarly journals Mature Embryo-Based in vitro Regeneration of Indica Rice Cultivars for High Frequency Plantlets Production

2017 ◽  
Vol 20 (2) ◽  
pp. 81-87
Author(s):  
HN Barman ◽  
ME Hoque ◽  
RK Roy ◽  
PL Biswas ◽  
MAI Khan ◽  
...  
Keyword(s):  
Callus Induction ◽  
High Frequency ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Mature Embryo ◽  
Ms Medium ◽  
Rice Varieties ◽  
Growing Medium ◽  
Regeneration Efficiency

The study was conducted at Biotechnology Division of Bangladesh Rice Research Institute (BRRI) to investigate the effects of plant growing medium and plant growth regulator (PGR) for the callus induction and high frequency plantlets regeneration of indica rice. Ten indica rice varieties viz. BR5, BR11, BRRI dhan28, BRRI dhan29, BRRI dhan33, BRRI dhan41, BRRI dhan47, BRRI dhan48, BRRI dhan49 and BRRI dhan50 were cultured on MS, N6 and LS media. The MS medium was found better for callus induction as compared to N6 and LS media. Among the tested varieties BRRI dhan48 induced the highest percent and best quality callus. Interaction effects of BRRI dhan48 to MS medium yielded 71.55% callus induction. The regeneration efficiency of BRRI dhan48 was tested on MS medium supplemented with different combination of NAA plus BAP and NAA plus kinetin. MS medium supplemented with 2.0 mg L-1 NAA and 2.0 mg L-1 Kn was found the best in respect of percent regenerated (76.67%) plantlet as well as for the growth of plantlets in vitro.Bangladesh Rice j. 2016, 20(2): 81-87

In vitro flowering of indica rice (Oryza sativa L. spp. indica)

2012 ◽  
Vol 48 (2) ◽  
pp. 259-264 ◽  
Author(s):  
Suriyan Cha-um ◽  
Thapanee Samphumphuang ◽  
Chalermpol Kirdmanee
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Flowering

scholarly journals Optimization of in vitro regeneration protocol for a popular Indica rice (Oryza sativa L. cv Swarna)

2016 ◽  
Vol 5 (08) ◽  
pp. 1395 ◽  
Author(s):  
Vijaya Naresh Juturu ◽  
Gopala Krishna Mekala ◽  
Mallikarjuna Garladinne ◽  
Puli Chandra Obul Reddy ◽  
Akila Chandra Sekhar*
Keyword(s):  
Callus Induction ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Rice Varieties ◽  
Regenerated Plants ◽  
Rooting Medium

Though regeneration system in rice has been very well established compare to other crop plants, the fact remains that, most of the indica rice varieties are still recalcitrant for regeneration and genetic transformation. Therefore, refinement of tissue culture protocol for generation of embryogenic calli and regeneration of the fertile plants from a single cell should be a pre requisite event for development of transgenic plants. Here, in this study we reported high frequency robust regeneration protocols for a popular Indica cultivar Swarna.Mature seeds were used as initial material as explants. Highest callus induction % was observed in MSCIMP medium containing 2.0 mg-1 2,4, D + 0.5 mg-1 Kn as phytohormonal combinations. In addition, maximum regeneration was observed in 2.0 mg-l KN + 0.5 mg-l NAA. Regenerated plants were shifted to rooting medium followed by polyhouse for hardening. The callus induction and regeneration reported in this study were well suited for transformation agronomical important genes or functional genomics studies.

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