GINGIVAL FLUID AS A POTENTIAL OBJECT FOR DIAGNOSTICS PROCESS

Metronidazole is the most popular representative of antiprotozoal medicines from the group of 5-nitroimidazoles. Metronidazole blocks the enzymes of alcohol dehydrogenase and acetaldehyde dehydrogenase, therefore when its joint taking with alcohol it is observed the strong intoxication syndrome and fatal poisonings too. Therefore metronidazole can be a potential object of chemical toxicological investigations. The purpose of our paper is to develop HPLC/UV-procedure of metronidazole quantification with application of the system of HPLC-analyzer MiLiChrome® A-0230 implemented in practice of forensic medical laboratories in Russia and Ukraine and carry out step-by-step validation of the developed procedure. Chromatographic conditions: Eluent A (0.2 M LiClO4 – 0.005 M HClO4) and Eluent B (acetonitrile) wereused as the mobile phase components; HPLC microcolumn Ø2×75 mm and ProntoSIL 120-5-C18 AQ, 5 μm were used as an analytical column; temperature was 40°С; flow rate was 100 μl/min; gradient elution mode was from 5% to 100% Eluent B for 40 min, then 100% Eluent B for 3 min; detection was performed at 277 nm. Retention time for metronidazole is 5.95 min. Since metronidazole is easy soluble and stable enough in the solutions of diluted alkalis 0.001 M sodium hydroxide solution has been proposed for preparation of the solutions in developing HPLC/UV-procedure of metronidazole quantification. Validation of the procedure has been carried out in the variants of the method of calibration curve and method of standard by such parameters as in process stability, linearity/calibration model, accuracy and precision within 3 different analytical runs using different batches of reagents and different glassware; experiments have been performed by three different analysts. New procedure of metronidazole quantitative determination by the method of HPLC/UV has been developed. Its validation has been carried out and acceptability for application has been shown.

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Plasminogen activating capacity in gingival fluid from deteriorating and stable jreiodontal pockets

Journal of Periodontal Research ◽

10.1034/j.1600-0765.2002.00325.x ◽

2002 ◽

Vol 37 (1) ◽

pp. 60-65 ◽

Cited By ~ 14

Author(s):

Anna Olofsson ◽

Lars Matsson ◽

Bertil Kinnby

Keyword(s):

Gingival Fluid

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The origin of gingival fluid

Journal of Theoretical Biology ◽

10.1016/0022-5193(74)90103-9 ◽

1974 ◽

Vol 47 (1) ◽

pp. 127-136 ◽

Cited By ~ 64

Author(s):

Michael C. Alfano

Keyword(s):

Gingival Fluid

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Effect of Brushing the Teeth with a Fluoride-Containing and Fluoridefree Dentifrice on the Gingiva of Dogs

Journal of Dental Research ◽

10.1177/00220345760550051801 ◽

1976 ◽

Vol 55 (5) ◽

pp. 825-828 ◽

Cited By ~ 3

Author(s):

L. Brayer ◽

H. Rennert ◽

I. Gedalia

Keyword(s):

Gingival Index ◽

Gingival Fluid

No retardation in the improvement of the gingival condition occurs after brushing the teeth with a dentifrice that contains fluoride. Gingival indexes and gingival fluid appeared to be related. The range of the gingival index was much narrower than that of the amount of gingival fluid.

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Microbial profile of grain liquid of dogs of different age groups

The Agrarian Scientific Journal ◽

10.28983/asj.y2019i8pp41-46 ◽

2019 ◽

pp. 41-46

Author(s):

Aleksandr Vladimirovich Krasnikov ◽

Ekaterina Sergeevna Krasnikova ◽

Tatiana Antonovna Chistyakova ◽

Daria Dmitrievna Morozova

Keyword(s):

Risk Factors ◽

Age Groups ◽

Practical Significance ◽

Microbial Composition ◽

Periodontal Tissues ◽

Microbial Profile ◽

Gingival Fluid

The article presents the results of a comparison of the microbial composition of the gingival fluid of dogs of different age groups. The practical significance of the work lies in the fact that the data obtained during the study allow us to predict the risk factors for damage to periodontal tissues, as well as to determine the choice of antiseptic and antibiotic, the duration of the course of their use.

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The interaction between structure and meaning in sentence comprehension: Recurrent neural networks and reading times

10.31234/osf.io/mks5y ◽

2019 ◽

Author(s):

Stefan L. Frank ◽

John Hoeks

Keyword(s):

Neural Network ◽

Neural Networks ◽

Sentence Processing ◽

Recurrent Neural Networks ◽

Sentence Comprehension ◽

Semantic Relations ◽

Garden Path ◽

Word Predictability ◽

Syntactic Ambiguities ◽

Potential Object

Recurrent neural network (RNN) models of sentence processing have recently displayed a remarkable ability to learn aspects of structure comprehension, as evidenced by their ability to account for reading times on sentences with local syntactic ambiguities (i.e., garden-path effects). Here, we investigate if these models can also simulate the effect of semantic appropriateness of the ambiguity's readings. RNNs-based estimates of surprisal of the disambiguating verb of sentences with an NP/S-coordination ambiguity (as in `The wizard guards the king and the princess protects ...') show identical patters to human reading times on the same sentences: Surprisal is higher on ambiguous structures than on their disambiguated counterparts and this effect is weaker, but not absent, in cases of poor thematic fit between the verb and its potential object (`The teacher baked the cake and the baker made ...'). These results show that an RNN is able to simultaneously learn about structural and semantic relations between words and suggest that garden-path phenomena may be more closely related to word predictability than traditionally assumed.

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