MINUTE ROTIFER DARI PERAIRAN ESTUARI SULAWESI UTARA DAN POTENSINYA SEBAGAI PAKAN LARVA IKAN

Penelitian ini bertujuan untuk mendapatkan minute rotifer (rotifer berukuran kecil) yang ada di perairan Sulawesi Utara yang memiliki potensi untuk dimanfaatkan sebagai pakan awal larva ikan laut. Sampling rotifer dilakukan di tujuh lokasi estuari yang berbeda dengan menggunakan plankton net (mata jaring 40 mm). Prosedur identifikasi rotifer didasarkan pada tampakan morfologi. Morfometri dilakukan di bawah mikroskop pembesaran 40-100x yang terhubung dengan sebuah komputer untuk visualisasi dan pengukuran. Tiga spesies minute rotifer berhasil diisolasi dari tiga lokasi estuari berbeda. Colurella sp. diisolasi dari sebuah kolam payau (salinitas 25 ppt) yang dipenuhi sampah rumah tangga di Tumpaan Kabupaten Minahasa. Lecane sp. cf Lecane quadridentata (Lecane) diisolasi dari sebuah tambak payau (salinitas 17 ppt) di Meras Kota Manado dan Lecane sp. cf Lecane papuana di muara sebuah sungai kecil (salinitas <3 ppt) di Tateli Kabupaten Minahasa. Semua minute rotifer yang ditemukan ini berhasil didomestikasi dan menunjukkan adaptasi positif dalam pemeliharaan berbasis mikroalga. Colurella sp, Lecane sp. cf L. quadridentata dan Lecane sp. cf L. papuana memiliki ukuran panjang lorika (PL) masing-masing (97,10 ± 3,58 mm, 130,83 ± 12,06 mm dan 118,70 ± 5,46 mm) sedangkan lebar lorika (55,37 ± 2,04 mm, 91,95 ± 10,58 mm dan 101,28 ± 6,623 mm) yang secara signifikan lebih kecil dari B. rotundiformis (PL 167,41 ± 9,10 mm dan LL 122,44 ± 7,29 mm) (p<0,05), sehingga berpotensi untuk dimanfaatkan sebagai pakan awal larva ikan laut yang membutuhkan pakan berukuran lebih kecil. Kata kunci: minute rotifer, Lecane sp, Colurella sp, larva This study aims to get minute rotifers (small rotifers) in the waters of North Sulawesi which has the potential to be used as starting food for marine fish larvae. The sampling for rotifers was conducted in seven different estuarine locations using plankton net (mesh size 40 mm). Rotifer identification procedure was based on morphological appearances. Morphometric observations were conducted under 40-100x magnification microscope connected to a computer for visualization and measurement. Three rotifer species were able to be isolated from three different estuarine locations. Colurella sp. was isolated from a brackish pond (salinity of 25 ppt) which were filled with household garbage in Tumpaan, Minahasa regency. Lecane sp. cf Lecane quadridentata (Lecane) was isolated from a brackish pond (salinity of 17 ppt) in Meras-Manado and Lecane sp. cf Lecane papuana at the estuary of a small river (salinity <3 ppt) in Tateli, Minahasa regency. All minute rotifers were successfully domesticated and showed positive adaptation in microalgae-based rearing. Colurella sp, Lecane sp. cf L. quadridentata and Lecane sp. cf L. papuana have lorica length (PL) of 97.10 ± 3.58 mm, 130.83 ± 12.06 mm, and 118.70 ± 5.46 mm, respectively, and lorica width (LL) of 55.37 mm ± 2.04, 91.95 ± 10.58 mm, and 101.28 ± 6.623 mm. They were significantly smaller than B. rotundiformis (PL of 167.41 ± 9.10 mm and LL 122.44 ± 7.29 mm) (p<0.05), which are commonly used in larva rearing. Thus these rotifers have potentials to be used as starting food for marine fish larvae that need a smaller size food. Keywords: Minute rotifer, Lecane sp, Colurella sp, larva

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Isolation, morphometry, and culture of Colurella sp. (Rotifera: Ploimida)

AQUATIC SCIENCE & MANAGEMENT ◽

10.35800/jasm.1.2.2013.7276 ◽

2013 ◽

Vol 1 (2) ◽

pp. 143

Author(s):

Petrus P Letsoin ◽

Henneke Pangkey ◽

Julius Sampekalo ◽

Inneke F.M Rumengan ◽

Stenly Wullur ◽

...

Keyword(s):

Body Size ◽

Marine Fish ◽

Fish Larvae ◽

Nannochloropsis Oculata ◽

Tropical Fish ◽

Sampling Location ◽

Larval Rearing ◽

Brachionus Rotundiformis ◽

Marine Fish Larvae ◽

Significant Difference

The rotifer Brachionus rotundiformis (total body length 240.59±10.24 μm, lorica length 175.28±9.18 μm, and lorica width 124.28±7.76μm) is commonly used as starter food in the larval rearing of marine fish. But, larvae of some marine tropical fish species required starter food with body size smaller than B. rotundiformis. The present study was aimed to isolate minute rotifers from nature and to assess the possibility of culturing these rotifers. Sampling of rotifers was conducted in an estuary of Mangket (Kema-Minut), using plankton net (mesh size 40 µm). A trial of culturing the rotifers was conducted at salinities of 10, 20 and 30 ppt by using a microalga, Nannochloropsis oculata. A species of rotifer identified as Colurella sp. (family Lepadellidae) was successfully isolated from the sampling location. Body size of Colurella sp. was extremely small (Total length 123.22±5.45 μm, lorica length 95.96±3.81 μm, and lorica width 53.57±3.11 μm), which were smaller than Brachionus rotundiformis SS-type as a conventional starter food for marine fish larvae. Results of culturing the minute rotifer Colurella sp. showed that the species grew well at salinities of 10, 20 and 30 ppt with no significant difference among treatments (ANOVA, p>0.05), indicating a potential use of minute rotifer Colurellasp. as starter food for marine fish larvae. Rotifera Branchionus rotundiformis (ukuran tubuh: panjang total 240,59±10,24 μm, panjang lorika 175,28±9,18 μm, dan lebar lorika 124,28±7,76μm) sering digunakan sebagai pakan awal pemeliharaan larva ikan laut. Namun, larva beberapa spesis ikan laut tropis membutuhkan pakan awal berukuran tubuh lebih kecil dari Branchionus rotundiformis. Penelitian ini bertujuan untuk mendapatkan minute rotifer dari alam (berukuran tubuh lebih kecil dari B. rotundiformis) dan menguji kemungkinan pemeliharaannya. Sampling rotifer dilakukan di perairan estuari Desa Mangket (Kema-Minut), menggunakan plankton net (ukuran mata jaring 40 µm). Uji coba pemeliharaan dilakukan pada salinitas (10, 20, dan 30 ppt) dengan menggunakan Nannochloropsis oculata. Satu spesies minute rotifer yang teridentifikasi sebagai Colurella sp. (family Lepadellidae) berhasil diisolasi dari lokasi sampling. Colurella sp. memiliki ukuran tubuh sangat kecil (panjang total [PT] 123,22±5,45 µm, panjang lorika [PL] 95,96±3,81 µm, dan lebar lorik [LL] 53,57±3,11 µm) yang mana lebih kecil dari Branchionus rotundiformis tipe-SS sebagai pakan awal larva ikan laut. Hasil uji coba pemeliharaan minute rotifer Colurella sp. menunjukkan bahwa spesis ini dapat tumbuh pada salinitas 10, 20, dan 30 ppt dengan perbedaan kepadatan populasi yang tidak signifikan antar perlakuan (Uji ANOVA, p > 0.05) mengindikasikan potensi pemanfaatan minute rotifer Colurella sp. sebagai pakan awal larva ikan laut.

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A Preliminary Survey on the Planktonic Biota in a Hypersaline Pond of Messolonghi Saltworks (W. Greece)

Diversity ◽

10.3390/d13060270 ◽

2021 ◽

Vol 13 (6) ◽

pp. 270

Author(s):

George N. Hotos

Keyword(s):

New Species ◽

Marine Fish ◽

Broad Spectrum ◽

Fish Larvae ◽

Live Food ◽

Preliminary Survey ◽

Genus Level ◽

Marine Fish Larvae ◽

Alternaria Sp ◽

Different Strains

During a survey in 2015, an impressive assemblage of organisms was found in a hypersaline pond of the Messolonghi saltworks. The salinity ranged between 50 and 180 ppt, and the organisms that were found fell into the categories of Cyanobacteria (17 species), Chlorophytes (4 species), Diatoms (23 species), Dinoflagellates (1 species), Protozoa (40 species), Rotifers (8 species), Copepods (1 species), Artemia sp., one nematode and Alternaria sp. (Fungi). Fabrea salina was the most prominent protist among all samples and salinities. This ciliate has the potential to be a live food candidate for marine fish larvae. Asteromonas gracilis proved to be a sturdy microalga, performing well in a broad spectrum of culture salinities. Most of the specimens were identified to the genus level only. Based on their morphology, as there are no relevant records in Greece, there is a possibility for some to be either new species or strikingly different strains of certain species recorded elsewhere.

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